A gene cluster involved in metal homeostasis in the cyanobacterium Synechocystis sp. strain PCC 6803

A gene cluster composed of nine open reading frames (ORFs) involved in Ni2+, Co2+, and Zn2+ sensing and tolerance in the cyanobacterium Synechocystis sp. Strain PCC 6803 has been identified. The cluster includes an Ni2+ response operon and a Co2+ response system, as well as a Zn2+ response system pr...

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Detalles Bibliográficos
Autores: García-Domínguez, Mario, López-Maury, Luis, Florencio, Francisco J., Reyes, José C.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2000
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/270053
Acceso en línea:http://hdl.handle.net/10261/270053
Access Level:acceso abierto
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spelling A gene cluster involved in metal homeostasis in the cyanobacterium Synechocystis sp. strain PCC 6803García-Domínguez, MarioLópez-Maury, LuisFlorencio, Francisco J.Reyes, José C.A gene cluster composed of nine open reading frames (ORFs) involved in Ni2+, Co2+, and Zn2+ sensing and tolerance in the cyanobacterium Synechocystis sp. Strain PCC 6803 has been identified. The cluster includes an Ni2+ response operon and a Co2+ response system, as well as a Zn2+ response system previously described. Expression of the Ni2+ response operon (nrs) was induced in the presence of Ni2+ and Co2+. Reduced Ni2+ tolerance was observed following disruption of two ORFs of the operon (nrsA and nrsD). We also show that the nrsD gene encodes a putative Ni2+ permease whose carboxy-terminal region is a metal binding domain. The Co2+ response system is composed of two divergently transcribed genes, corR and corT, mutants of which showed decreased Co2+ tolerance. Additionally, corR mutants showed an absence of Co2+-dependent induction of corT, indicating that CorR is a transcriptional activator of corT. To our knowledge, CorR is the first Co2+-sensing transcription factor described. Our data suggest that this region of the Synechocystis sp. strain PCC 6803 genome is involved in sensing and homeostasis of Ni2+, Co2+, and Zn2+.We thank the Kazusa DNA Research Institute and S. Tabata for providing CS1377 cosmid DNA. We are grateful to E. Santero for critical reading of the manuscript. M. Garcı´a-Domı´nguez was the recipient of a fellowship from the Spanish Ministerio de Educacio´n y Cultura. This work was supported by grant PB97-0732 from DGESIC and by Junta de Andalucı´a (group CV1-0112).American Society for MicrobiologyMinisterio de Educación y Cultura (España)Junta de AndalucíaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2022202220002022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/270053reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1128/JB.182.6.1507-1514.2000Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2700532026-05-22T06:33:51Z
dc.title.none.fl_str_mv A gene cluster involved in metal homeostasis in the cyanobacterium Synechocystis sp. strain PCC 6803
title A gene cluster involved in metal homeostasis in the cyanobacterium Synechocystis sp. strain PCC 6803
spellingShingle A gene cluster involved in metal homeostasis in the cyanobacterium Synechocystis sp. strain PCC 6803
García-Domínguez, Mario
title_short A gene cluster involved in metal homeostasis in the cyanobacterium Synechocystis sp. strain PCC 6803
title_full A gene cluster involved in metal homeostasis in the cyanobacterium Synechocystis sp. strain PCC 6803
title_fullStr A gene cluster involved in metal homeostasis in the cyanobacterium Synechocystis sp. strain PCC 6803
title_full_unstemmed A gene cluster involved in metal homeostasis in the cyanobacterium Synechocystis sp. strain PCC 6803
title_sort A gene cluster involved in metal homeostasis in the cyanobacterium Synechocystis sp. strain PCC 6803
dc.creator.none.fl_str_mv García-Domínguez, Mario
López-Maury, Luis
Florencio, Francisco J.
Reyes, José C.
author García-Domínguez, Mario
author_facet García-Domínguez, Mario
López-Maury, Luis
Florencio, Francisco J.
Reyes, José C.
author_role author
author2 López-Maury, Luis
Florencio, Francisco J.
Reyes, José C.
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Educación y Cultura (España)
Junta de Andalucía
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description A gene cluster composed of nine open reading frames (ORFs) involved in Ni2+, Co2+, and Zn2+ sensing and tolerance in the cyanobacterium Synechocystis sp. Strain PCC 6803 has been identified. The cluster includes an Ni2+ response operon and a Co2+ response system, as well as a Zn2+ response system previously described. Expression of the Ni2+ response operon (nrs) was induced in the presence of Ni2+ and Co2+. Reduced Ni2+ tolerance was observed following disruption of two ORFs of the operon (nrsA and nrsD). We also show that the nrsD gene encodes a putative Ni2+ permease whose carboxy-terminal region is a metal binding domain. The Co2+ response system is composed of two divergently transcribed genes, corR and corT, mutants of which showed decreased Co2+ tolerance. Additionally, corR mutants showed an absence of Co2+-dependent induction of corT, indicating that CorR is a transcriptional activator of corT. To our knowledge, CorR is the first Co2+-sensing transcription factor described. Our data suggest that this region of the Synechocystis sp. strain PCC 6803 genome is involved in sensing and homeostasis of Ni2+, Co2+, and Zn2+.
publishDate 2000
dc.date.none.fl_str_mv 2000
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/270053
url http://hdl.handle.net/10261/270053
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1128/JB.182.6.1507-1514.2000
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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