Production of an extensive sunflower protein hydrolysate by sequential hydrolysis with endo- and exo-proteases

5 pages, 2 figures, 2 tables.

Detalhes bibliográficos
Autores: Villanueva, Álvaro, Clemente, Alfonso, Bautista-Gallego, J., Millán, Francisco
Formato: artículo
Fecha de publicación:1999
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/22009
Acesso em linha:http://hdl.handle.net/10261/22009
Access Level:acceso abierto
Palavra-chave:Alcalase
Flavourzyme
Protein hydrolysate
Protein isolate
Sunflower
Aislado proteico
Alcalasa
Flavorzima
Girasol
Hidrolizado proteico
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oai_identifier_str oai:digital.csic.es:10261/22009
network_acronym_str ES
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repository_id_str
spelling Production of an extensive sunflower protein hydrolysate by sequential hydrolysis with endo- and exo-proteasesProducción de un hidrolizado proteico extenso de girasol mediante hidrólisis secuencial con endo- y exo-proteasasVillanueva, ÁlvaroClemente, AlfonsoBautista-Gallego, J.Millán, FranciscoAlcalaseFlavourzymeProtein hydrolysateProtein isolateSunflowerAislado proteicoAlcalasaFlavorzimaGirasolHidrolizado proteico5 pages, 2 figures, 2 tables.[EN] A high quality protein isolate has been obtained from defatted sunflower meal by alkaline extraction and isoelectric precipitation. Protein content was increased from 31.2 % in the defatted flour to 97 % in the protein isolate. The percentages of fiber, soluble sugars, polyphenols and residual lipids in the protein isolate were reduced to more than 90 % with respect to the defatted meal. The protein isolate was used as starting material for the generation of an extensive enzymatic protein hydrolysate. The hydrolysis was carried out in a pH stat using sequentially an endo-protease (Alcalase) and an exo-protease (Flavourzyme). The protein hydrolysate, with a degree of hydrolysis of 50.7 %, was white and non bitter.[ES] Se ha obtenido un aislado proteico de alta calidad a partir de harina desengrasada de girasol, mediante extracción alcalina y precipitación isoeléctrica. Se incrementó el contenido proteico desde un 31.2 % en la harina desengrasada hasta un 97 % en el aislado proteico. Los porcentajes de fibra, azúcares solubles, polifenoles y lípidos residuales se redujeron en más del 90 % en el aislado proteico respecto a la harina desengrasada. Se usó el aislado proteico como material de partida para la producción de un hidrolizado enzimático proteico extenso. La hidrólisis se realizó en un reactor usando secuencialmente una endo-proteasa (Alcalasa) y una exo-proteasa (Flavorzima). El hidrolizado proteico, con un grado de hidrólisis del 50.7 %, era blanco y no presentaba amargor.This work was supported by CICYT Grant AL198-0766.Peer reviewedConsejo Superior de Investigaciones Científicas (España)201020101999info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_65011326232 bytesapplication/pdfhttp://hdl.handle.net/10261/22009reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.3989/gya.1999.v50.i6.697info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/220092026-05-22T06:33:51Z
dc.title.none.fl_str_mv Production of an extensive sunflower protein hydrolysate by sequential hydrolysis with endo- and exo-proteases
Producción de un hidrolizado proteico extenso de girasol mediante hidrólisis secuencial con endo- y exo-proteasas
title Production of an extensive sunflower protein hydrolysate by sequential hydrolysis with endo- and exo-proteases
spellingShingle Production of an extensive sunflower protein hydrolysate by sequential hydrolysis with endo- and exo-proteases
Villanueva, Álvaro
Alcalase
Flavourzyme
Protein hydrolysate
Protein isolate
Sunflower
Aislado proteico
Alcalasa
Flavorzima
Girasol
Hidrolizado proteico
title_short Production of an extensive sunflower protein hydrolysate by sequential hydrolysis with endo- and exo-proteases
title_full Production of an extensive sunflower protein hydrolysate by sequential hydrolysis with endo- and exo-proteases
title_fullStr Production of an extensive sunflower protein hydrolysate by sequential hydrolysis with endo- and exo-proteases
title_full_unstemmed Production of an extensive sunflower protein hydrolysate by sequential hydrolysis with endo- and exo-proteases
title_sort Production of an extensive sunflower protein hydrolysate by sequential hydrolysis with endo- and exo-proteases
dc.creator.none.fl_str_mv Villanueva, Álvaro
Clemente, Alfonso
Bautista-Gallego, J.
Millán, Francisco
author Villanueva, Álvaro
author_facet Villanueva, Álvaro
Clemente, Alfonso
Bautista-Gallego, J.
Millán, Francisco
author_role author
author2 Clemente, Alfonso
Bautista-Gallego, J.
Millán, Francisco
author2_role author
author
author
dc.subject.none.fl_str_mv Alcalase
Flavourzyme
Protein hydrolysate
Protein isolate
Sunflower
Aislado proteico
Alcalasa
Flavorzima
Girasol
Hidrolizado proteico
topic Alcalase
Flavourzyme
Protein hydrolysate
Protein isolate
Sunflower
Aislado proteico
Alcalasa
Flavorzima
Girasol
Hidrolizado proteico
description 5 pages, 2 figures, 2 tables.
publishDate 1999
dc.date.none.fl_str_mv 1999
2010
2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/22009
url http://hdl.handle.net/10261/22009
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.3989/gya.1999.v50.i6.697
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1326232 bytes
application/pdf
dc.publisher.none.fl_str_mv Consejo Superior de Investigaciones Científicas (España)
publisher.none.fl_str_mv Consejo Superior de Investigaciones Científicas (España)
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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