Estudio de proteínas reguladoras del ciclo celular en psoriasis mediante matrices tisulares
[EN] Background. Psoriasis is a chronic inflammatory disease characterized histologically by hyperproliferation and aberrant differentiation of epidermal keratinocytes . Keratinocytes undergo alterations in cell cycle regulation may be demonstrable by studying their expression regulatory proteins. T...
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| Tipo de recurso: | tesis doctoral |
| Fecha de publicación: | 2013 |
| País: | España |
| Institución: | Universidad de Salamanca (USAL) |
| Repositorio: | GREDOS. Repositorio Institucional de la Universidad de Salamanca |
| OAI Identifier: | oai:gredos.usal.es:10366/123046 |
| Acceso en línea: | http://hdl.handle.net/10366/123046 |
| Access Level: | acceso abierto |
| Palabra clave: | Academic dissertations Dermatology Tesis y disertaciones académicas Universidad de Salamanca (España) Dermatología 3201.06 Dermatología |
| Sumario: | [EN] Background. Psoriasis is a chronic inflammatory disease characterized histologically by hyperproliferation and aberrant differentiation of epidermal keratinocytes . Keratinocytes undergo alterations in cell cycle regulation may be demonstrable by studying their expression regulatory proteins. The expression patterns of these proteins could be used as a diagnostic tool in cases where the clinical , histopathology and clinicopathologic correlation is not enough. Objective . To evaluate the immunohistochemical expression of 11 cell cycle proteins in skin samples from patients with psoriasis and other inflammatory dermatoses compare . Methods . By using the technique of tissue arrays cyclin E expression , cyclin D1 , cyclin A, p63 , p53 , p27 , p21 , CDK1 , CDK2, Ki67 and Bcl-2 in 70 samples from patients with psoriasis ( cases ) was evaluated and compared 63 biopsies from other skin diseases : lupus erythematosus , pityriasis rosea, pityriasis lichenoides and eczema ( controls). Results . Ten of the eleven cell cycle proteins studied in our series showed statistically significant differences between cases (psoriasis ) and controls ( no psoriasis) . These associations are listed in the accompanying table. p21 , p53 and cyclin D1 revealed inverse association , ie positivity was more frequent in the control group. The remaining proteins studied showed a pattern of more frequent in cases ( psoriasis) positivity , with p63 , cyclin E, CDK2 and p27 proteins with greater intensity of association with the case group. Conclusions . Psoriasis presents immunohistochemical expression patterns of the studied cell cycle proteins different from other skin inflammatory diseases. These findings support the theory of the abnormal regulation of the cell cycle of keratinocytes in psoriasis affected . These differences in the patterns of immunohistochemical expression could be used as a diagnostic tool in cases where the clinicopathological correlation is not enough. |
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