Effects of grape seed procyanidin extract on proliferation and apoptosis in pancreatic cells
Procyanidins have been reported to modulate glucose homeostasis and β-cell functionality. However, their effects on pancreatic cell mass remain unknown. Therefore, this doctoral thesis focused on the study of the effects of a grape seed procyanidin extract (GSPE) on proliferation and apoptosis proce...
| Autor: | |
|---|---|
| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2013 |
| País: | España |
| Institución: | CBUC, CESCA |
| Repositorio: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/132854 |
| Acceso en línea: | http://hdl.handle.net/10803/132854 |
| Access Level: | acceso abierto |
| Palabra clave: | Procyanidins Pancreatic cells Apoptosis Proliferation 577 663/664 |
| Sumario: | Procyanidins have been reported to modulate glucose homeostasis and β-cell functionality. However, their effects on pancreatic cell mass remain unknown. Therefore, this doctoral thesis focused on the study of the effects of a grape seed procyanidin extract (GSPE) on proliferation and apoptosis processes in pancreatic cells. Our results indicate that although the extract did not affect these processes under healthy conditions, it modulated β-cell proliferation and apoptosis under altered conditions. In vitro, GSPE enhanced the pro-apoptotic effects of high glucose and were antiproliferative under high glucose, insulin, and palmitate levels in the INS-1E cell line. In vivo, the effects of the extract depended on the dose, the duration of the treatment, and/or the gender. However, the extract tended to counteract the deleterious effects of the cafeteria diet or the Zucker Fatty genotype. Concerning the adenocarcinoma cell line MIA PaCa-2, GSPE exerted antiproliferative and pro-apoptotic effects, demonstrating anti-carcinogenic activity. |
|---|