Heterochromatin Is Not the Only Place for satDNAs: The High Diversity of satDNAs in the Euchromatin of the Beetle Chrysolina americana (Coleoptera, Chrysomelidae)

Rico Porras, José M.; Mora, Pablo; Palomeque, Teresa; Montiel Jiménez, Eugenia Elisabet; Cabral de Mello, Diogo C.; Lorite, Pedro

Heterochromatin Is Not the Only Place for satDNAs: The High Diversity of satDNAs in the Euchromatin of the Beetle Chrysolina americana (Coleoptera, Chrysomelidae)

The satellitome of the beetle Chrysolina americana Linneo, 1758 has been characterized through chromosomal analysis, genomic sequencing, and bioinformatics tools. C-banding reveals the presence of constitutive heterochromatin blocks enriched in A+T content, primarily located in pericentromeric regio...

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Detalles Bibliográficos
Autores:	Rico Porras, José M., Mora, Pablo, Palomeque, Teresa, Montiel Jiménez, Eugenia Elisabet, Cabral de Mello, Diogo C., Lorite, Pedro
Tipo de recurso:	artículo
Fecha de publicación:	2024
País:	España
Institución:	Universidad Autónoma de Madrid
Repositorio:	Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:	inglés
OAI Identifier:	oai:repositorio.uam.es:10486/718094
Acceso en línea:	http://hdl.handle.net/10486/718094 https://dx.doi.org/10.3390/genes15040395
Access Level:	acceso abierto
Palabra clave:	CHRISMAPP chromosome in silico mapping euchromatin fluorescence in situ hybridization genome evolution heterochromatin karyotype repetitive DNA satellite DNA satellitome Biología y Biomedicina / Biología

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spelling	Heterochromatin Is Not the Only Place for satDNAs: The High Diversity of satDNAs in the Euchromatin of the Beetle Chrysolina americana (Coleoptera, Chrysomelidae)Rico Porras, José M.Mora, PabloPalomeque, TeresaMontiel Jiménez, Eugenia ElisabetCabral de Mello, Diogo C.Lorite, PedroCHRISMAPPchromosome in silico mappingeuchromatinfluorescence in situ hybridizationgenome evolutionheterochromatinkaryotyperepetitive DNAsatellite DNAsatellitomeBiología y Biomedicina / BiologíaThe satellitome of the beetle Chrysolina americana Linneo, 1758 has been characterized through chromosomal analysis, genomic sequencing, and bioinformatics tools. C-banding reveals the presence of constitutive heterochromatin blocks enriched in A+T content, primarily located in pericentromeric regions. Furthermore, a comprehensive satellitome analysis unveils the extensive diversity of satellite DNA families within the genome of C. americana. Using fluorescence in situ hybridization techniques and the innovative CHRISMAPP approach, we precisely map the localization of satDNA families on assembled chromosomes, providing insights into their organization and distribution patterns. Among the 165 identified satDNA families, only three of them exhibit a remarkable amplification and accumulation, forming large blocks predominantly in pericentromeric regions. In contrast, the remaining, less abundant satDNA families are dispersed throughout euchromatic regions, challenging the traditional association of satDNA with heterochromatin. Overall, our findings underscore the complexity of repetitive DNA elements in the genome of C. americana and emphasize the need for further exploration to elucidate their functional significance and evolutionary implicationsThis work was supported by Universidad de Jaén (through the program “Plan de Apoyo a la Investigación 2021–2022, Acción 1”)Multidisciplinary Digital Publishing Institute (MDPI)Departamento de BiologíaFacultad de Ciencias20242024-03-22research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/718094https://dx.doi.org/10.3390/genes15040395reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7180942026-06-23T12:46:27Z
dc.title.none.fl_str_mv	Heterochromatin Is Not the Only Place for satDNAs: The High Diversity of satDNAs in the Euchromatin of the Beetle Chrysolina americana (Coleoptera, Chrysomelidae)
title	Heterochromatin Is Not the Only Place for satDNAs: The High Diversity of satDNAs in the Euchromatin of the Beetle Chrysolina americana (Coleoptera, Chrysomelidae)
spellingShingle	Heterochromatin Is Not the Only Place for satDNAs: The High Diversity of satDNAs in the Euchromatin of the Beetle Chrysolina americana (Coleoptera, Chrysomelidae) Rico Porras, José M. CHRISMAPP chromosome in silico mapping euchromatin fluorescence in situ hybridization genome evolution heterochromatin karyotype repetitive DNA satellite DNA satellitome Biología y Biomedicina / Biología
title_short	Heterochromatin Is Not the Only Place for satDNAs: The High Diversity of satDNAs in the Euchromatin of the Beetle Chrysolina americana (Coleoptera, Chrysomelidae)
title_full	Heterochromatin Is Not the Only Place for satDNAs: The High Diversity of satDNAs in the Euchromatin of the Beetle Chrysolina americana (Coleoptera, Chrysomelidae)
title_fullStr	Heterochromatin Is Not the Only Place for satDNAs: The High Diversity of satDNAs in the Euchromatin of the Beetle Chrysolina americana (Coleoptera, Chrysomelidae)
title_full_unstemmed	Heterochromatin Is Not the Only Place for satDNAs: The High Diversity of satDNAs in the Euchromatin of the Beetle Chrysolina americana (Coleoptera, Chrysomelidae)
title_sort	Heterochromatin Is Not the Only Place for satDNAs: The High Diversity of satDNAs in the Euchromatin of the Beetle Chrysolina americana (Coleoptera, Chrysomelidae)
dc.creator.none.fl_str_mv	Rico Porras, José M. Mora, Pablo Palomeque, Teresa Montiel Jiménez, Eugenia Elisabet Cabral de Mello, Diogo C. Lorite, Pedro
author	Rico Porras, José M.
author_facet	Rico Porras, José M. Mora, Pablo Palomeque, Teresa Montiel Jiménez, Eugenia Elisabet Cabral de Mello, Diogo C. Lorite, Pedro
author_role	author
author2	Mora, Pablo Palomeque, Teresa Montiel Jiménez, Eugenia Elisabet Cabral de Mello, Diogo C. Lorite, Pedro
author2_role	author author author author author
dc.contributor.none.fl_str_mv	Departamento de Biología Facultad de Ciencias
dc.subject.none.fl_str_mv	CHRISMAPP chromosome in silico mapping euchromatin fluorescence in situ hybridization genome evolution heterochromatin karyotype repetitive DNA satellite DNA satellitome Biología y Biomedicina / Biología
topic	CHRISMAPP chromosome in silico mapping euchromatin fluorescence in situ hybridization genome evolution heterochromatin karyotype repetitive DNA satellite DNA satellitome Biología y Biomedicina / Biología
description	The satellitome of the beetle Chrysolina americana Linneo, 1758 has been characterized through chromosomal analysis, genomic sequencing, and bioinformatics tools. C-banding reveals the presence of constitutive heterochromatin blocks enriched in A+T content, primarily located in pericentromeric regions. Furthermore, a comprehensive satellitome analysis unveils the extensive diversity of satellite DNA families within the genome of C. americana. Using fluorescence in situ hybridization techniques and the innovative CHRISMAPP approach, we precisely map the localization of satDNA families on assembled chromosomes, providing insights into their organization and distribution patterns. Among the 165 identified satDNA families, only three of them exhibit a remarkable amplification and accumulation, forming large blocks predominantly in pericentromeric regions. In contrast, the remaining, less abundant satDNA families are dispersed throughout euchromatic regions, challenging the traditional association of satDNA with heterochromatin. Overall, our findings underscore the complexity of repetitive DNA elements in the genome of C. americana and emphasize the need for further exploration to elucidate their functional significance and evolutionary implications
publishDate	2024
dc.date.none.fl_str_mv	2024 2024-03-22
dc.type.none.fl_str_mv	research article http://purl.org/coar/resource_type/c_2df8fbb1 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	http://hdl.handle.net/10486/718094 https://dx.doi.org/10.3390/genes15040395
url	http://hdl.handle.net/10486/718094 https://dx.doi.org/10.3390/genes15040395
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	Multidisciplinary Digital Publishing Institute (MDPI)
publisher.none.fl_str_mv	Multidisciplinary Digital Publishing Institute (MDPI)
dc.source.none.fl_str_mv	reponame:Biblos-e Archivo. Repositorio Institucional de la UAM instname:Universidad Autónoma de Madrid
instname_str	Universidad Autónoma de Madrid
reponame_str	Biblos-e Archivo. Repositorio Institucional de la UAM
collection	Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15,811543

Heterochromatin Is Not the Only Place for satDNAs: The High Diversity of satDNAs in the Euchromatin of the Beetle Chrysolina americana (Coleoptera, Chrysomelidae)

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