Tear <SUP>1</SUP>H Nuclear Magnetic Resonance-Based Metabolomics Application to the Molecular Diagnosis of Aqueous Tear Deficiency and Meibomian Gland Dysfunction

Purpose: Meibomian gland dysfunction (MGD) is a major cause of signs and symptoms related to dry eyes (DE) and eyelid inflammation. We investigated the composition of human tears by metabolomic approaches in patients with aqueous tear deficiency and MGD. Methods: Participants in this prospective, ca...

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Autores: Benítez Del Castillo JM, Pinazo-Duran MD, Sanz-González SM, Muñoz-Hernández AM, Garcia-Medina JJ, Zanón-Moreno V
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
Repositorio:r-FISABIO. Repositorio Institucional de Producción Científica
OAI Identifier:oai:fisabio.fundanetsuite.com:p9344
Acceso en línea:https://fisabio.portalinvestigacion.com/publicaciones/9344
Access Level:acceso abierto
Palabra clave:Dry eyes
Meibomian gland dysfunction
Aqueous tear deficiency metabolomic fingerprint
Tears
Biomarkers
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spelling Tear <SUP>1</SUP>H Nuclear Magnetic Resonance-Based Metabolomics Application to the Molecular Diagnosis of Aqueous Tear Deficiency and Meibomian Gland DysfunctionBenítez Del Castillo JMPinazo-Duran MDSanz-González SMMuñoz-Hernández AMGarcia-Medina JJZanón-Moreno VDry eyesMeibomian gland dysfunctionAqueous tear deficiency metabolomic fingerprintTearsBiomarkersPurpose: Meibomian gland dysfunction (MGD) is a major cause of signs and symptoms related to dry eyes (DE) and eyelid inflammation. We investigated the composition of human tears by metabolomic approaches in patients with aqueous tear deficiency and MGD. Methods: Participants in this prospective, case-control pilot study were split into patients with aqueous tear deficiency and MGD (DE-MGD [n = 15]) and healthy controls (CG; n = 20). Personal interviews, ocular surface disease index (OSDI), and ophthalmic examinations were performed. Reflex tears collected by capillarity were processed to H-1 nuclear magnetic resonance (NMR) spectroscopy and quantitative data analysis to identify molecules by spectra comparison to library entries of purified standards and/or unknown entities. Statistical analyses were made by the SPSS 22.0 program. Results: Chemometric analysis and H-1 NMR spectra comparison revealed the presence of 60 metabolites in tears. Differentiating features were evident in the NMR spectra of the 2 clinical groups, characterized by significant upregulation of phenylalanine, glycerol, and isoleucine, and downregulation of glycoproteins, leucine, and -CH3 lipids, as compared to the CG. The H-1 NMR metabolomic analyses of human tears confirmed the applicability of this platform with high predictive accuracy/reliability. Conclusions: Our key distinctive findings support that DE-MGD induces tear metabolomics profile changes. Metabolites contributing to a higher separation from the CG can presumably be used, in the foreseeable future, as DE-MGD biomarkers for better managing the diagnosis and therapy of this disease.KARGER2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fisabio.portalinvestigacion.com/publicaciones/9344OPHTHALMIC RESEARCHISSN: 00303747ISSNe: 14230259reponame:r-FISABIO. Repositorio Institucional de Producción Científicainstname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)Inglésinfo:eu-repo/semantics/openAccessoai:fisabio.fundanetsuite.com:p93442026-06-11T12:45:17Z
dc.title.none.fl_str_mv Tear <SUP>1</SUP>H Nuclear Magnetic Resonance-Based Metabolomics Application to the Molecular Diagnosis of Aqueous Tear Deficiency and Meibomian Gland Dysfunction
title Tear <SUP>1</SUP>H Nuclear Magnetic Resonance-Based Metabolomics Application to the Molecular Diagnosis of Aqueous Tear Deficiency and Meibomian Gland Dysfunction
spellingShingle Tear <SUP>1</SUP>H Nuclear Magnetic Resonance-Based Metabolomics Application to the Molecular Diagnosis of Aqueous Tear Deficiency and Meibomian Gland Dysfunction
Benítez Del Castillo JM
Dry eyes
Meibomian gland dysfunction
Aqueous tear deficiency metabolomic fingerprint
Tears
Biomarkers
title_short Tear <SUP>1</SUP>H Nuclear Magnetic Resonance-Based Metabolomics Application to the Molecular Diagnosis of Aqueous Tear Deficiency and Meibomian Gland Dysfunction
title_full Tear <SUP>1</SUP>H Nuclear Magnetic Resonance-Based Metabolomics Application to the Molecular Diagnosis of Aqueous Tear Deficiency and Meibomian Gland Dysfunction
title_fullStr Tear <SUP>1</SUP>H Nuclear Magnetic Resonance-Based Metabolomics Application to the Molecular Diagnosis of Aqueous Tear Deficiency and Meibomian Gland Dysfunction
title_full_unstemmed Tear <SUP>1</SUP>H Nuclear Magnetic Resonance-Based Metabolomics Application to the Molecular Diagnosis of Aqueous Tear Deficiency and Meibomian Gland Dysfunction
title_sort Tear <SUP>1</SUP>H Nuclear Magnetic Resonance-Based Metabolomics Application to the Molecular Diagnosis of Aqueous Tear Deficiency and Meibomian Gland Dysfunction
dc.creator.none.fl_str_mv Benítez Del Castillo JM
Pinazo-Duran MD
Sanz-González SM
Muñoz-Hernández AM
Garcia-Medina JJ
Zanón-Moreno V
author Benítez Del Castillo JM
author_facet Benítez Del Castillo JM
Pinazo-Duran MD
Sanz-González SM
Muñoz-Hernández AM
Garcia-Medina JJ
Zanón-Moreno V
author_role author
author2 Pinazo-Duran MD
Sanz-González SM
Muñoz-Hernández AM
Garcia-Medina JJ
Zanón-Moreno V
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Dry eyes
Meibomian gland dysfunction
Aqueous tear deficiency metabolomic fingerprint
Tears
Biomarkers
topic Dry eyes
Meibomian gland dysfunction
Aqueous tear deficiency metabolomic fingerprint
Tears
Biomarkers
description Purpose: Meibomian gland dysfunction (MGD) is a major cause of signs and symptoms related to dry eyes (DE) and eyelid inflammation. We investigated the composition of human tears by metabolomic approaches in patients with aqueous tear deficiency and MGD. Methods: Participants in this prospective, case-control pilot study were split into patients with aqueous tear deficiency and MGD (DE-MGD [n = 15]) and healthy controls (CG; n = 20). Personal interviews, ocular surface disease index (OSDI), and ophthalmic examinations were performed. Reflex tears collected by capillarity were processed to H-1 nuclear magnetic resonance (NMR) spectroscopy and quantitative data analysis to identify molecules by spectra comparison to library entries of purified standards and/or unknown entities. Statistical analyses were made by the SPSS 22.0 program. Results: Chemometric analysis and H-1 NMR spectra comparison revealed the presence of 60 metabolites in tears. Differentiating features were evident in the NMR spectra of the 2 clinical groups, characterized by significant upregulation of phenylalanine, glycerol, and isoleucine, and downregulation of glycoproteins, leucine, and -CH3 lipids, as compared to the CG. The H-1 NMR metabolomic analyses of human tears confirmed the applicability of this platform with high predictive accuracy/reliability. Conclusions: Our key distinctive findings support that DE-MGD induces tear metabolomics profile changes. Metabolites contributing to a higher separation from the CG can presumably be used, in the foreseeable future, as DE-MGD biomarkers for better managing the diagnosis and therapy of this disease.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fisabio.portalinvestigacion.com/publicaciones/9344
url https://fisabio.portalinvestigacion.com/publicaciones/9344
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv KARGER
publisher.none.fl_str_mv KARGER
dc.source.none.fl_str_mv OPHTHALMIC RESEARCH
ISSN: 00303747
ISSNe: 14230259
reponame:r-FISABIO. Repositorio Institucional de Producción Científica
instname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
instname_str Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
reponame_str r-FISABIO. Repositorio Institucional de Producción Científica
collection r-FISABIO. Repositorio Institucional de Producción Científica
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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