First data on Ornithodoros moubata Aquaporins: structural, phylogenetic and immunogenic characterisation as vaccine targets

21 páginas, 4 tablas, 4 figuras,11 figuras suplementarias, 11 tablas suplementarias

Detalhes bibliográficos
Autores: Pérez Sánchez, Ricardo, Cano Argüelles, Ana Laura, González-Sánchez, María, Oleaga, Ana
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/273044
Acesso em linha:http://hdl.handle.net/10261/273044
Access Level:acceso abierto
Palavra-chave:Soft ticks
Ornithodoros moubata
Aquaporins
Tick vaccines
Antigenic peptides
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spelling First data on Ornithodoros moubata Aquaporins: structural, phylogenetic and immunogenic characterisation as vaccine targetsPérez Sánchez, RicardoCano Argüelles, Ana LauraGonzález-Sánchez, MaríaOleaga, AnaSoft ticksOrnithodoros moubataAquaporinsTick vaccinesAntigenic peptides21 páginas, 4 tablas, 4 figuras,11 figuras suplementarias, 11 tablas suplementariasOrnithodoros moubata transmits African swine fever and human relapsing fever in Africa. The elimination of O. moubata populations from anthropic environments is expected to improve the prevention and control of these diseases. Tick vaccines have emerged as a sustainable method for tick control, and tick aquaporins (AQPs) are promising targets for tick vaccines due to their vital functions, immunogenicity and ease of access by neutralising host antibodies. This study aimed at the systematic identification of the AQPs expressed by O. moubata (OmAQPs) and their characterisation as vaccine targets. Therefore, AQP coding sequences were recovered from available transcriptomic datasets, followed by PCR amplification, cloning, sequence verification and the analysis of the AQP protein structure and epitope exposure. Seven OmAQPs were identified and characterised: six were aquaglyceroporins, and one was a water-specific aquaporin. All of these were expressed in the salivary glands and midgut and only three in the coxal glands. Epitope exposure analysis identified three extracellular domains in each AQP, which concentrate overlapping B and T cell epitopes, making them interesting vaccine targets. Based on these domain sequences, a set of ten antigenic peptides was designed, which showed adequate properties to be produced and tested in pilot vaccine trialsThis research was funded by the project “RTI2018-098297-B-I00” (MCIU/AEI/FEDER, UE), granted by the Spanish Ministry of Science, Innovation and Universities, the State Research Agency (AEI) and the European Regional Development Fund (ERDF); and project “CLU-2019-05-IRNASA/CSIC Unit of Excellence”, granted by the Junta de Castilla y León and co-financed by the European Union (ERDF “Europe drives our growth”).Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia, Innovación y Universidades (España)Junta de Castilla y LeónPérez Sánchez, Ricardo [0000-0001-8875-439X]Cano Argüelles, Ana Laura [0000-0002-1056-8439]González Sánchez, María [0000-0002-7176-3721]Oleaga, Ana [0000-0002-8019-7354]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/273044reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-098297-B-I00https://doi.org/10.3390/pathogens1106069Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2730442026-05-22T06:33:51Z
dc.title.none.fl_str_mv First data on Ornithodoros moubata Aquaporins: structural, phylogenetic and immunogenic characterisation as vaccine targets
title First data on Ornithodoros moubata Aquaporins: structural, phylogenetic and immunogenic characterisation as vaccine targets
spellingShingle First data on Ornithodoros moubata Aquaporins: structural, phylogenetic and immunogenic characterisation as vaccine targets
Pérez Sánchez, Ricardo
Soft ticks
Ornithodoros moubata
Aquaporins
Tick vaccines
Antigenic peptides
title_short First data on Ornithodoros moubata Aquaporins: structural, phylogenetic and immunogenic characterisation as vaccine targets
title_full First data on Ornithodoros moubata Aquaporins: structural, phylogenetic and immunogenic characterisation as vaccine targets
title_fullStr First data on Ornithodoros moubata Aquaporins: structural, phylogenetic and immunogenic characterisation as vaccine targets
title_full_unstemmed First data on Ornithodoros moubata Aquaporins: structural, phylogenetic and immunogenic characterisation as vaccine targets
title_sort First data on Ornithodoros moubata Aquaporins: structural, phylogenetic and immunogenic characterisation as vaccine targets
dc.creator.none.fl_str_mv Pérez Sánchez, Ricardo
Cano Argüelles, Ana Laura
González-Sánchez, María
Oleaga, Ana
author Pérez Sánchez, Ricardo
author_facet Pérez Sánchez, Ricardo
Cano Argüelles, Ana Laura
González-Sánchez, María
Oleaga, Ana
author_role author
author2 Cano Argüelles, Ana Laura
González-Sánchez, María
Oleaga, Ana
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Junta de Castilla y León
Pérez Sánchez, Ricardo [0000-0001-8875-439X]
Cano Argüelles, Ana Laura [0000-0002-1056-8439]
González Sánchez, María [0000-0002-7176-3721]
Oleaga, Ana [0000-0002-8019-7354]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Soft ticks
Ornithodoros moubata
Aquaporins
Tick vaccines
Antigenic peptides
topic Soft ticks
Ornithodoros moubata
Aquaporins
Tick vaccines
Antigenic peptides
description 21 páginas, 4 tablas, 4 figuras,11 figuras suplementarias, 11 tablas suplementarias
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/273044
url http://hdl.handle.net/10261/273044
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-098297-B-I00
https://doi.org/10.3390/pathogens1106069
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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