Screening of cyanotoxin producing genes in Ecuadorian freshwater systems

This study pursued the detection of cyr and mcy genes to assess the presence of cylindrospemopsin (CN) and microcystin (MC) potential producers in Ecuadorian water bodies. Methods Environmental DNA (eDNA) was extracted from eight water bodies and one wastewater treatment plant (WWTP) from Ecuador. A...

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Detalles Bibliográficos
Autores: Ballesteros Redondo, María Isabel, Cruz, Sara de la, Rojas, Michelle, Salazar, Génesis, Martínez-Fresneda, Miguel, Castillejo, Pablo
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/102964
Acceso en línea:https://hdl.handle.net/20.500.14352/102964
Access Level:acceso abierto
Palabra clave:579.25
Cylindrospermopsin
Microcystin
Nested PCR
Andes
Amazon
Microbiología (Biología)
Genética
2414 Microbiología
2409 Genética
id ES_ed2b53e22e496e1dcb19b41e080754e1
oai_identifier_str oai:docta.ucm.es:20.500.14352/102964
network_acronym_str ES
network_name_str España
repository_id_str
spelling Screening of cyanotoxin producing genes in Ecuadorian freshwater systemsBallesteros Redondo, María IsabelCruz, Sara de laRojas, MichelleSalazar, GénesisMartínez-Fresneda, MiguelCastillejo, Pablo579.25CylindrospermopsinMicrocystinNested PCRAndesAmazonMicrobiología (Biología)Genética2414 Microbiología2409 GenéticaThis study pursued the detection of cyr and mcy genes to assess the presence of cylindrospemopsin (CN) and microcystin (MC) potential producers in Ecuadorian water bodies. Methods Environmental DNA (eDNA) was extracted from eight water bodies and one wastewater treatment plant (WWTP) from Ecuador. A nested PCR was designed to amplify mcyB, cyrE, and cyrJ genes in these environmental samples. PCR products were sequenced and blasted against GenBank database. Results Potential CN producers were found in seven water bodies and the WWTP. cyrE amplification revealed three variants belonging to Raphidopsis and Aphanizmenon species and one for cyrJ with around 90% identity with Raphidiopsis and Oscillatoria species. Four water bodies presented the same variant for mcyB similar to Microcystis panniformis with 99% of identity. Conclusions This study contributes new data on the presence of toxic cyanobacteria strains and provides new molecular tools to assess cyanotoxin hazards in Ecuadorian water bodies.Associação Brasileira de LimnologiaUniversidad Complutense de Madrid20222022-01-0120222022-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/102964reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1029642026-06-02T12:44:21Z
dc.title.none.fl_str_mv Screening of cyanotoxin producing genes in Ecuadorian freshwater systems
title Screening of cyanotoxin producing genes in Ecuadorian freshwater systems
spellingShingle Screening of cyanotoxin producing genes in Ecuadorian freshwater systems
Ballesteros Redondo, María Isabel
579.25
Cylindrospermopsin
Microcystin
Nested PCR
Andes
Amazon
Microbiología (Biología)
Genética
2414 Microbiología
2409 Genética
title_short Screening of cyanotoxin producing genes in Ecuadorian freshwater systems
title_full Screening of cyanotoxin producing genes in Ecuadorian freshwater systems
title_fullStr Screening of cyanotoxin producing genes in Ecuadorian freshwater systems
title_full_unstemmed Screening of cyanotoxin producing genes in Ecuadorian freshwater systems
title_sort Screening of cyanotoxin producing genes in Ecuadorian freshwater systems
dc.creator.none.fl_str_mv Ballesteros Redondo, María Isabel
Cruz, Sara de la
Rojas, Michelle
Salazar, Génesis
Martínez-Fresneda, Miguel
Castillejo, Pablo
author Ballesteros Redondo, María Isabel
author_facet Ballesteros Redondo, María Isabel
Cruz, Sara de la
Rojas, Michelle
Salazar, Génesis
Martínez-Fresneda, Miguel
Castillejo, Pablo
author_role author
author2 Cruz, Sara de la
Rojas, Michelle
Salazar, Génesis
Martínez-Fresneda, Miguel
Castillejo, Pablo
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 579.25
Cylindrospermopsin
Microcystin
Nested PCR
Andes
Amazon
Microbiología (Biología)
Genética
2414 Microbiología
2409 Genética
topic 579.25
Cylindrospermopsin
Microcystin
Nested PCR
Andes
Amazon
Microbiología (Biología)
Genética
2414 Microbiología
2409 Genética
description This study pursued the detection of cyr and mcy genes to assess the presence of cylindrospemopsin (CN) and microcystin (MC) potential producers in Ecuadorian water bodies. Methods Environmental DNA (eDNA) was extracted from eight water bodies and one wastewater treatment plant (WWTP) from Ecuador. A nested PCR was designed to amplify mcyB, cyrE, and cyrJ genes in these environmental samples. PCR products were sequenced and blasted against GenBank database. Results Potential CN producers were found in seven water bodies and the WWTP. cyrE amplification revealed three variants belonging to Raphidopsis and Aphanizmenon species and one for cyrJ with around 90% identity with Raphidiopsis and Oscillatoria species. Four water bodies presented the same variant for mcyB similar to Microcystis panniformis with 99% of identity. Conclusions This study contributes new data on the presence of toxic cyanobacteria strains and provides new molecular tools to assess cyanotoxin hazards in Ecuadorian water bodies.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-01-01
2022
2022-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/102964
url https://hdl.handle.net/20.500.14352/102964
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Limnologia
publisher.none.fl_str_mv Associação Brasileira de Limnologia
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869423430185517056
score 15,298079

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