Oxidative damage, genetic and epigenetic alterations in hexavalent chromium exposed workers - A cross-sectional study within the SafeChrom project

Background . Hexavalent chromium (Cr(VI)) is a lung cancer carcinogen. However, the genotoxic and mutagenic effects of Cr(VI) in humans at low-to-moderate occupational exposure levels are unknown. This study aims to investigate the relationship between occupational exposure to Cr(VI) and the presenc...

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Autores: Jiang, Zheshun, Runkel, Agneta, Lindh, Christian, Kukka, Aimonen, Catalán, Julia, Pineda, Daniela, Lundh, Thomas, Vogel, Ulla, Saber, Anne T., Tondel, Martin, Engfeldt, Malin, Krais, Annette M., Broberg, Karin
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Recursos:Universidad de Zaragoza
Repositorio:Zaguán. Repositorio Digital de la Universidad de Zaragoza
OAI Identifier:oai:zaguan.unizar.es:161905
Acesso em linha:http://zaguan.unizar.es/record/161905
Access Level:acceso abierto
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spelling Oxidative damage, genetic and epigenetic alterations in hexavalent chromium exposed workers - A cross-sectional study within the SafeChrom projectJiang, ZheshunRunkel, AgnetaLindh, ChristianKukka, AimonenCatalán, JuliaPineda, DanielaLundh, ThomasVogel, UllaSaber, Anne T.Tondel, MartinEngfeldt, MalinKrais, Annette M.Broberg, KarinBackground . Hexavalent chromium (Cr(VI)) is a lung cancer carcinogen. However, the genotoxic and mutagenic effects of Cr(VI) in humans at low-to-moderate occupational exposure levels are unknown. This study aims to investigate the relationship between occupational exposure to Cr(VI) and the presence of oxidative damage, genetic and epigenetic alterations. Methods. We included 113 Cr(VI) exposed workers in 14 companies and 72 controls recruited within the SafeChrom project. Cr(VI) was measured in inhalable dust and total chromium in urine (U-Cr) and red blood cells (RBC-Cr). Analysed effect biomarkers included urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG), micronuclei in peripheral blood reticulocytes (MNRET), blood relative mitochondrial DNA copy number (mtDNA-cn), relative telomere length (TL), and blood DNA methylation of four lung cancer-related genes (F2RL3, LINE-1, MGMT promoter and SEMA4B). Results. The median inhalable Cr(VI) concentration among the exposed workers was 0.11 μg/m3 (5th-95th percentile: 0.02–8.44). Exposed workers showed higher 8-OHdG, TL, and MGMT promoter methylation levels and lower mtDNA-cn and MNRET compared with controls. Company-based differences in biomarkers were observed. Univariate analysis showed that TL was positively correlated with U-Cr, and 8-OHdG and MGMT promoter methylation were positively correlated with RBC-Cr. Multivariate analyses with adjustment for possible confounders showed higher 8-OHdG, TL, and MGMT promoter methylation in exposed workers compared with controls. Conclusions. Low-to-moderate Cr(VI) exposure was associated with higher oxidative stress, longer telomeres and epigenetic alterations, changes that previously have been linked to lung cancer risk. This study highlights the molecular impacts of Cr(VI) exposure, underscoring the importance of reducing the exposure to Cr(VI).2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://zaguan.unizar.es/record/161905reponame:Zaguán. Repositorio Digital de la Universidad de Zaragozainstname:Universidad de ZaragozaInglésinfo:eu-repo/semantics/openAccessoai:zaguan.unizar.es:1619052026-05-29T13:59:51Z
dc.title.none.fl_str_mv Oxidative damage, genetic and epigenetic alterations in hexavalent chromium exposed workers - A cross-sectional study within the SafeChrom project
title Oxidative damage, genetic and epigenetic alterations in hexavalent chromium exposed workers - A cross-sectional study within the SafeChrom project
spellingShingle Oxidative damage, genetic and epigenetic alterations in hexavalent chromium exposed workers - A cross-sectional study within the SafeChrom project
Jiang, Zheshun
title_short Oxidative damage, genetic and epigenetic alterations in hexavalent chromium exposed workers - A cross-sectional study within the SafeChrom project
title_full Oxidative damage, genetic and epigenetic alterations in hexavalent chromium exposed workers - A cross-sectional study within the SafeChrom project
title_fullStr Oxidative damage, genetic and epigenetic alterations in hexavalent chromium exposed workers - A cross-sectional study within the SafeChrom project
title_full_unstemmed Oxidative damage, genetic and epigenetic alterations in hexavalent chromium exposed workers - A cross-sectional study within the SafeChrom project
title_sort Oxidative damage, genetic and epigenetic alterations in hexavalent chromium exposed workers - A cross-sectional study within the SafeChrom project
dc.creator.none.fl_str_mv Jiang, Zheshun
Runkel, Agneta
Lindh, Christian
Kukka, Aimonen
Catalán, Julia
Pineda, Daniela
Lundh, Thomas
Vogel, Ulla
Saber, Anne T.
Tondel, Martin
Engfeldt, Malin
Krais, Annette M.
Broberg, Karin
author Jiang, Zheshun
author_facet Jiang, Zheshun
Runkel, Agneta
Lindh, Christian
Kukka, Aimonen
Catalán, Julia
Pineda, Daniela
Lundh, Thomas
Vogel, Ulla
Saber, Anne T.
Tondel, Martin
Engfeldt, Malin
Krais, Annette M.
Broberg, Karin
author_role author
author2 Runkel, Agneta
Lindh, Christian
Kukka, Aimonen
Catalán, Julia
Pineda, Daniela
Lundh, Thomas
Vogel, Ulla
Saber, Anne T.
Tondel, Martin
Engfeldt, Malin
Krais, Annette M.
Broberg, Karin
author2_role author
author
author
author
author
author
author
author
author
author
author
author
description Background . Hexavalent chromium (Cr(VI)) is a lung cancer carcinogen. However, the genotoxic and mutagenic effects of Cr(VI) in humans at low-to-moderate occupational exposure levels are unknown. This study aims to investigate the relationship between occupational exposure to Cr(VI) and the presence of oxidative damage, genetic and epigenetic alterations. Methods. We included 113 Cr(VI) exposed workers in 14 companies and 72 controls recruited within the SafeChrom project. Cr(VI) was measured in inhalable dust and total chromium in urine (U-Cr) and red blood cells (RBC-Cr). Analysed effect biomarkers included urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG), micronuclei in peripheral blood reticulocytes (MNRET), blood relative mitochondrial DNA copy number (mtDNA-cn), relative telomere length (TL), and blood DNA methylation of four lung cancer-related genes (F2RL3, LINE-1, MGMT promoter and SEMA4B). Results. The median inhalable Cr(VI) concentration among the exposed workers was 0.11 μg/m3 (5th-95th percentile: 0.02–8.44). Exposed workers showed higher 8-OHdG, TL, and MGMT promoter methylation levels and lower mtDNA-cn and MNRET compared with controls. Company-based differences in biomarkers were observed. Univariate analysis showed that TL was positively correlated with U-Cr, and 8-OHdG and MGMT promoter methylation were positively correlated with RBC-Cr. Multivariate analyses with adjustment for possible confounders showed higher 8-OHdG, TL, and MGMT promoter methylation in exposed workers compared with controls. Conclusions. Low-to-moderate Cr(VI) exposure was associated with higher oxidative stress, longer telomeres and epigenetic alterations, changes that previously have been linked to lung cancer risk. This study highlights the molecular impacts of Cr(VI) exposure, underscoring the importance of reducing the exposure to Cr(VI).
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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dc.identifier.none.fl_str_mv http://zaguan.unizar.es/record/161905
url http://zaguan.unizar.es/record/161905
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
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dc.source.none.fl_str_mv reponame:Zaguán. Repositorio Digital de la Universidad de Zaragoza
instname:Universidad de Zaragoza
instname_str Universidad de Zaragoza
reponame_str Zaguán. Repositorio Digital de la Universidad de Zaragoza
collection Zaguán. Repositorio Digital de la Universidad de Zaragoza
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