Characterization of porcine milk oligosaccharides during early lactation and their relation to the fecal microbiome

The composition of porcine milk oligosaccharides (PMO) was analyzed during early lactation and their relation to piglet gut microbiome was investigated. Pigs are considered ideal intestinal models to simulate humans because of the striking similarity in intestinal physiopathology to humans. The evol...

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Autores: Salcedo, Jaime, Frese, S.A., Mills, D.A., Barile, D.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/415239
Acceso en línea:http://hdl.handle.net/10261/415239
https://api.elsevier.com/content/abstract/scopus_id/84981743029
Access Level:acceso abierto
Palabra clave:Gut microbiota
Oligosaccharide
Porcine milk
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spelling Characterization of porcine milk oligosaccharides during early lactation and their relation to the fecal microbiomeSalcedo, JaimeFrese, S.A.Mills, D.A.Barile, D.Gut microbiotaOligosaccharidePorcine milkThe composition of porcine milk oligosaccharides (PMO) was analyzed during early lactation and their relation to piglet gut microbiome was investigated. Pigs are considered ideal intestinal models to simulate humans because of the striking similarity in intestinal physiopathology to humans. The evolution of PMO was investigated in the milk from 3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum by Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer (Agilent Technologies, Santa Clara, CA). Previously sequenced metagenome libraries were reanalyzed to examine changes with specific gut bacterial populations. Over 30 oligosaccharides (OS) were identified in the milk, with 3'-sialyllactose, lacto-N-tetraose, α1-3,β1-4-d-galactotriose, 2'-fucosyllactose, and 6'-sialyllactose being the most abundant species (accounting for ~70% of the total OS). Porcine milk had lower OS diversity (number of unique structures) than human milk, and appeared closer to bovine and caprine milk. In agreement with previous studies, only 3 fucosylated OS were identified. Surprisingly, their contribution to total OS abundance was greater than in bovine milk (9 vs. 1%). Indeed, fucosylated PMO increased during lactation, mirroring a similar trend observed for neutral and type I OS content during early lactation. Taken together, these results suggest that, in terms of abundance, PMO are closer to human milk than other domestic species, such as bovine and caprine milks. Metagenomic sequencing revealed that fucose-consuming bacterial taxa in the gut microbiota of piglets were qualitatively but not quantitatively different between nursing and weaning stages, suggesting that both the composition and structure of dietary glycans may play a critical role in shaping the distal gut microbiome. The similarity of both intestinal physiopathology and milk OS composition in human and porcine species suggests similar effects on gastrointestinal development of early nutrition, reinforcing the use of the pig intestinal model to simulate human intestinal models in the clinical setting.This work was supported by the Bill and Melinda Gates Foundation (Seattle, WA), National Institutes of Health awards R01AT007079 and R01AT008759 (Bethesda, MD), and the Peter J. Shields Endowed Chair in Dairy Food Science (University of California–Davis). SAF was supported by National Institutes of Health Postdoctoral Fellowship F32AT008533.Peer reviewedElsevier202620262016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/415239https://api.elsevier.com/content/abstract/scopus_id/84981743029reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésJournal of dairy sciencehttps://doi.org/10.3168/jds.2016-10966Noinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4152392026-05-22T06:33:51Z
dc.title.none.fl_str_mv Characterization of porcine milk oligosaccharides during early lactation and their relation to the fecal microbiome
title Characterization of porcine milk oligosaccharides during early lactation and their relation to the fecal microbiome
spellingShingle Characterization of porcine milk oligosaccharides during early lactation and their relation to the fecal microbiome
Salcedo, Jaime
Gut microbiota
Oligosaccharide
Porcine milk
title_short Characterization of porcine milk oligosaccharides during early lactation and their relation to the fecal microbiome
title_full Characterization of porcine milk oligosaccharides during early lactation and their relation to the fecal microbiome
title_fullStr Characterization of porcine milk oligosaccharides during early lactation and their relation to the fecal microbiome
title_full_unstemmed Characterization of porcine milk oligosaccharides during early lactation and their relation to the fecal microbiome
title_sort Characterization of porcine milk oligosaccharides during early lactation and their relation to the fecal microbiome
dc.creator.none.fl_str_mv Salcedo, Jaime
Frese, S.A.
Mills, D.A.
Barile, D.
author Salcedo, Jaime
author_facet Salcedo, Jaime
Frese, S.A.
Mills, D.A.
Barile, D.
author_role author
author2 Frese, S.A.
Mills, D.A.
Barile, D.
author2_role author
author
author
dc.subject.none.fl_str_mv Gut microbiota
Oligosaccharide
Porcine milk
topic Gut microbiota
Oligosaccharide
Porcine milk
description The composition of porcine milk oligosaccharides (PMO) was analyzed during early lactation and their relation to piglet gut microbiome was investigated. Pigs are considered ideal intestinal models to simulate humans because of the striking similarity in intestinal physiopathology to humans. The evolution of PMO was investigated in the milk from 3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum by Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer (Agilent Technologies, Santa Clara, CA). Previously sequenced metagenome libraries were reanalyzed to examine changes with specific gut bacterial populations. Over 30 oligosaccharides (OS) were identified in the milk, with 3'-sialyllactose, lacto-N-tetraose, α1-3,β1-4-d-galactotriose, 2'-fucosyllactose, and 6'-sialyllactose being the most abundant species (accounting for ~70% of the total OS). Porcine milk had lower OS diversity (number of unique structures) than human milk, and appeared closer to bovine and caprine milk. In agreement with previous studies, only 3 fucosylated OS were identified. Surprisingly, their contribution to total OS abundance was greater than in bovine milk (9 vs. 1%). Indeed, fucosylated PMO increased during lactation, mirroring a similar trend observed for neutral and type I OS content during early lactation. Taken together, these results suggest that, in terms of abundance, PMO are closer to human milk than other domestic species, such as bovine and caprine milks. Metagenomic sequencing revealed that fucose-consuming bacterial taxa in the gut microbiota of piglets were qualitatively but not quantitatively different between nursing and weaning stages, suggesting that both the composition and structure of dietary glycans may play a critical role in shaping the distal gut microbiome. The similarity of both intestinal physiopathology and milk OS composition in human and porcine species suggests similar effects on gastrointestinal development of early nutrition, reinforcing the use of the pig intestinal model to simulate human intestinal models in the clinical setting.
publishDate 2016
dc.date.none.fl_str_mv 2016
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/415239
https://api.elsevier.com/content/abstract/scopus_id/84981743029
url http://hdl.handle.net/10261/415239
https://api.elsevier.com/content/abstract/scopus_id/84981743029
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Journal of dairy science
https://doi.org/10.3168/jds.2016-10966
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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