Ammonium acetate as a novel buffer for highly selective robust urinary HPLC-ICP-MS arsenic speciation methodology

Ammonium acetate is employed in order to develop a novel HPLC-ICP-MS arsenic speciation methodology applicable to six arsenic species, i.e, AC, AB, AsIII, AsV, DMA and MMA. The most predominant species in the toxicological field are covered in a 30-min chromatogram with reproducible and repeatabilit...

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Bibliographic Details
Authors: Rodríguez, P. F., López Colón, José Luis, Mendoza, J. L. de, Martín Aranda, Rosa María
Format: article
Publication Date:2021
Country:España
Institution:Universidad Nacional de Educación a Distancia
Repository:e-spacio. Repositorio Institucional de la UNED
Language:English
OAI Identifier:oai:e-spacio.uned.es:20.500.14468/12766
Online Access:https://hdl.handle.net/20.500.14468/12766
Access Level:Closed access
Keyword:Urinary
Ammonium Acetate
Arsenic
Speciation
HPLC-ICP-MS
Description
Summary:Ammonium acetate is employed in order to develop a novel HPLC-ICP-MS arsenic speciation methodology applicable to six arsenic species, i.e, AC, AB, AsIII, AsV, DMA and MMA. The most predominant species in the toxicological field are covered in a 30-min chromatogram with reproducible and repeatability peak area ratio. Moreover, typical problems from traditional methods are sorted out by using a robust, high-selective and 75ArCl+ interference-free methodology. Chromatographic and detector optimization ensures low LOQs for each species with acceptable precision and accuracy values obtained using four urinary arsenic speciation PTS enabling to be useful for sub ng mL−1 arsenic exposure assessments.