Blood Rheological Characterization of β-Thalassemia Trait and Iron Deficiency Anemia Using Front Microrheometry
The purpose of this work is to develop a hematocrit-independent method for the detection of beta-thalassemia trait (β-TT) and iron deficiency anemia (IDA), through the rheological characterization of whole blood samples from different donors. The results obtained herein are the basis for the develop...
| Autores: | , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2072/531270 |
| Acceso en línea: | http://hdl.handle.net/2072/531270 |
| Access Level: | acceso abierto |
| Palabra clave: | Anemia beta-thalassemia trait blood rheology hemorheology iron deficiency (anemia) microfluidics microrheometer rheology |
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Blood Rheological Characterization of β-Thalassemia Trait and Iron Deficiency Anemia Using Front MicrorheometryMéndez-Mora, L.Cabello-Fusarés, M.Ferré-Torres, J.Riera-Llobet, C.Krishnevskaya, E.Trejo-Soto, C.Payán-Pernía, S.Hernández-Rodríguez, I.Morales-Indiano, C.Alarcón, T.Vives-Corrons, J.-L.Hernandez-Machado, A.Anemiabeta-thalassemia traitblood rheologyhemorheologyiron deficiency (anemia)microfluidicsmicrorheometerrheologyThe purpose of this work is to develop a hematocrit-independent method for the detection of beta-thalassemia trait (β-TT) and iron deficiency anemia (IDA), through the rheological characterization of whole blood samples from different donors. The results obtained herein are the basis for the development of a front microrheometry point-of-care device for the diagnosis and clinical follow-up of β-TT patients suffering hematological diseases and alterations in the morphology of the red blood cell (RBC). The viscosity is calculated as a function of the mean front velocity by detecting the sample fluid-air interface advancing through a microfluidic channel. Different viscosity curves are obtained for healthy donors, β-TT and IDA samples. A mathematical model is introduced to compare samples of distinct hematocrit, classifying the viscosity curve patterns with respect to the health condition of blood. The viscosity of the fluid at certain shear rate values varies depending on several RBC factors such as shape and size, hemoglobin (Hb) content, membrane rigidity and hematocrit concentration. Blood and plasma from healthy donors are used as reference. To validate their potential clinical value as a diagnostic tool, the viscosity results are compared to those obtained by the gold-standard method for RBC deformability evaluation, the Laser-Optical Rotational Red Cell Analyzer (LoRRCA). Copyright © 2021 Méndez-Mora, Cabello-Fusarés, Ferré-Torres, Riera-Llobet, Krishnevskaya, Trejo-Soto, Payán-Pernía, Hernández-Rodríguez, Morales-Indiano, Alarcón, Vives-Corrons and Hernandez-Machado.2019-1-TR01-KA202-076789, FIS2016-78883-C2-1P, MDM-2014-0445, MTM2015-71509-C2-1-R; 2017 SGR-1061, PID2019-106063GB-100; Precast/Prestressed Concrete Institute, PCI: MEC80180021; Generalitat de Catalunya; Agència de Gestió d'Ajuts Universitaris i de Recerca, AGAURFrontiers Media S.A.2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion13 p.application/pdfhttp://hdl.handle.net/2072/531270RECERCAT (Dipòsit de la Recerca de Catalunya)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésFrontiers in PhysiologyL'accés als continguts d'aquest document queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2072/5312702026-05-29T05:05:01Z |
| dc.title.none.fl_str_mv |
Blood Rheological Characterization of β-Thalassemia Trait and Iron Deficiency Anemia Using Front Microrheometry |
| title |
Blood Rheological Characterization of β-Thalassemia Trait and Iron Deficiency Anemia Using Front Microrheometry |
| spellingShingle |
Blood Rheological Characterization of β-Thalassemia Trait and Iron Deficiency Anemia Using Front Microrheometry Méndez-Mora, L. Anemia beta-thalassemia trait blood rheology hemorheology iron deficiency (anemia) microfluidics microrheometer rheology |
| title_short |
Blood Rheological Characterization of β-Thalassemia Trait and Iron Deficiency Anemia Using Front Microrheometry |
| title_full |
Blood Rheological Characterization of β-Thalassemia Trait and Iron Deficiency Anemia Using Front Microrheometry |
| title_fullStr |
Blood Rheological Characterization of β-Thalassemia Trait and Iron Deficiency Anemia Using Front Microrheometry |
| title_full_unstemmed |
Blood Rheological Characterization of β-Thalassemia Trait and Iron Deficiency Anemia Using Front Microrheometry |
| title_sort |
Blood Rheological Characterization of β-Thalassemia Trait and Iron Deficiency Anemia Using Front Microrheometry |
| dc.creator.none.fl_str_mv |
Méndez-Mora, L. Cabello-Fusarés, M. Ferré-Torres, J. Riera-Llobet, C. Krishnevskaya, E. Trejo-Soto, C. Payán-Pernía, S. Hernández-Rodríguez, I. Morales-Indiano, C. Alarcón, T. Vives-Corrons, J.-L. Hernandez-Machado, A. |
| author |
Méndez-Mora, L. |
| author_facet |
Méndez-Mora, L. Cabello-Fusarés, M. Ferré-Torres, J. Riera-Llobet, C. Krishnevskaya, E. Trejo-Soto, C. Payán-Pernía, S. Hernández-Rodríguez, I. Morales-Indiano, C. Alarcón, T. Vives-Corrons, J.-L. Hernandez-Machado, A. |
| author_role |
author |
| author2 |
Cabello-Fusarés, M. Ferré-Torres, J. Riera-Llobet, C. Krishnevskaya, E. Trejo-Soto, C. Payán-Pernía, S. Hernández-Rodríguez, I. Morales-Indiano, C. Alarcón, T. Vives-Corrons, J.-L. Hernandez-Machado, A. |
| author2_role |
author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Anemia beta-thalassemia trait blood rheology hemorheology iron deficiency (anemia) microfluidics microrheometer rheology |
| topic |
Anemia beta-thalassemia trait blood rheology hemorheology iron deficiency (anemia) microfluidics microrheometer rheology |
| description |
The purpose of this work is to develop a hematocrit-independent method for the detection of beta-thalassemia trait (β-TT) and iron deficiency anemia (IDA), through the rheological characterization of whole blood samples from different donors. The results obtained herein are the basis for the development of a front microrheometry point-of-care device for the diagnosis and clinical follow-up of β-TT patients suffering hematological diseases and alterations in the morphology of the red blood cell (RBC). The viscosity is calculated as a function of the mean front velocity by detecting the sample fluid-air interface advancing through a microfluidic channel. Different viscosity curves are obtained for healthy donors, β-TT and IDA samples. A mathematical model is introduced to compare samples of distinct hematocrit, classifying the viscosity curve patterns with respect to the health condition of blood. The viscosity of the fluid at certain shear rate values varies depending on several RBC factors such as shape and size, hemoglobin (Hb) content, membrane rigidity and hematocrit concentration. Blood and plasma from healthy donors are used as reference. To validate their potential clinical value as a diagnostic tool, the viscosity results are compared to those obtained by the gold-standard method for RBC deformability evaluation, the Laser-Optical Rotational Red Cell Analyzer (LoRRCA). Copyright © 2021 Méndez-Mora, Cabello-Fusarés, Ferré-Torres, Riera-Llobet, Krishnevskaya, Trejo-Soto, Payán-Pernía, Hernández-Rodríguez, Morales-Indiano, Alarcón, Vives-Corrons and Hernandez-Machado. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/2072/531270 |
| url |
http://hdl.handle.net/2072/531270 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Frontiers in Physiology |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
13 p. application/pdf |
| dc.publisher.none.fl_str_mv |
Frontiers Media S.A. |
| publisher.none.fl_str_mv |
Frontiers Media S.A. |
| dc.source.none.fl_str_mv |
RECERCAT (Dipòsit de la Recerca de Catalunya) reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| instname_str |
Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| reponame_str |
Recercat. Dipósit de la Recerca de Catalunya |
| collection |
Recercat. Dipósit de la Recerca de Catalunya |
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1869423385531908096 |
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15,812429 |