Three-Photon Infrared Stimulation of Endogenous Neuroreceptors in Vivo.

To interrogate neural circuits and crack their codes, in vivo brain activity imaging must be combined with spatiotemporally precise stimulation in three dimensions using genetic or pharmacological specificity. This challenge requires deep penetration and focusing as provided by infrared light and mu...

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Autores: Sortino R, Cunquero M, Castro-Olvera G, Gelabert R, Moreno M, Riefolo F, Matera C, Fernàndez-Castillo N, Agnetta L, Decker M, Lluch JM, Hernando J, Loza-Alvarez P, Gorostiza P
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Fundació Sant Joan de Déu
Repositorio:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
OAI Identifier:oai:fsjd.fundanetsuite.com:p25130
Acceso en línea:https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=25130
Access Level:acceso abierto
Palabra clave:Azobenzene
Multiphoton Excitation
Muscarinic Neuromodulation
Photopharmacology
Two-Photon Lithography and Polymerization
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spelling Three-Photon Infrared Stimulation of Endogenous Neuroreceptors in Vivo.Sortino RCunquero MCastro-Olvera GGelabert RMoreno MRiefolo FMatera CFernàndez-Castillo NAgnetta LDecker MLluch JMHernando JLoza-Alvarez PGorostiza PAzobenzeneMultiphoton ExcitationMuscarinic NeuromodulationPhotopharmacologyTwo-Photon Lithography and PolymerizationTo interrogate neural circuits and crack their codes, in vivo brain activity imaging must be combined with spatiotemporally precise stimulation in three dimensions using genetic or pharmacological specificity. This challenge requires deep penetration and focusing as provided by infrared light and multiphoton excitation, and has promoted two-photon photopharmacology and optogenetics. However, three-photon brain stimulation in vivo remains to be demonstrated. We report the regulation of neuronal activity in zebrafish larvae by three-photon excitation of a photoswitchable muscarinic agonist at 50 pM, a billion-fold lower concentration than used for uncaging, and with mid-infrared light of 1560 nm, the longest reported photoswitch wavelength. Robust, physiologically relevant photoresponses allow modulating brain activity in wild-type animals with spatiotemporal and pharmacological precision. Computational calculations predict that azobenzene-based ligands have high three-photon absorption cross-section and can be used directly with pulsed infrared light. The expansion of three-photon pharmacology will deeply impact basic neurobiology and neuromodulation phototherapies.WILEY-V C H VERLAG GMBH2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=25130ANGEWANDTE CHEMIE-INTERNATIONAL EDITIONISSN: 14337851ISSNe: 15213773reponame:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déuinstname:Fundació Sant Joan de DéuInglésinfo:eu-repo/semantics/openAccessoai:fsjd.fundanetsuite.com:p251302026-05-27T12:37:41Z
dc.title.none.fl_str_mv Three-Photon Infrared Stimulation of Endogenous Neuroreceptors in Vivo.
title Three-Photon Infrared Stimulation of Endogenous Neuroreceptors in Vivo.
spellingShingle Three-Photon Infrared Stimulation of Endogenous Neuroreceptors in Vivo.
Sortino R
Azobenzene
Multiphoton Excitation
Muscarinic Neuromodulation
Photopharmacology
Two-Photon Lithography and Polymerization
title_short Three-Photon Infrared Stimulation of Endogenous Neuroreceptors in Vivo.
title_full Three-Photon Infrared Stimulation of Endogenous Neuroreceptors in Vivo.
title_fullStr Three-Photon Infrared Stimulation of Endogenous Neuroreceptors in Vivo.
title_full_unstemmed Three-Photon Infrared Stimulation of Endogenous Neuroreceptors in Vivo.
title_sort Three-Photon Infrared Stimulation of Endogenous Neuroreceptors in Vivo.
dc.creator.none.fl_str_mv Sortino R
Cunquero M
Castro-Olvera G
Gelabert R
Moreno M
Riefolo F
Matera C
Fernàndez-Castillo N
Agnetta L
Decker M
Lluch JM
Hernando J
Loza-Alvarez P
Gorostiza P
author Sortino R
author_facet Sortino R
Cunquero M
Castro-Olvera G
Gelabert R
Moreno M
Riefolo F
Matera C
Fernàndez-Castillo N
Agnetta L
Decker M
Lluch JM
Hernando J
Loza-Alvarez P
Gorostiza P
author_role author
author2 Cunquero M
Castro-Olvera G
Gelabert R
Moreno M
Riefolo F
Matera C
Fernàndez-Castillo N
Agnetta L
Decker M
Lluch JM
Hernando J
Loza-Alvarez P
Gorostiza P
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Azobenzene
Multiphoton Excitation
Muscarinic Neuromodulation
Photopharmacology
Two-Photon Lithography and Polymerization
topic Azobenzene
Multiphoton Excitation
Muscarinic Neuromodulation
Photopharmacology
Two-Photon Lithography and Polymerization
description To interrogate neural circuits and crack their codes, in vivo brain activity imaging must be combined with spatiotemporally precise stimulation in three dimensions using genetic or pharmacological specificity. This challenge requires deep penetration and focusing as provided by infrared light and multiphoton excitation, and has promoted two-photon photopharmacology and optogenetics. However, three-photon brain stimulation in vivo remains to be demonstrated. We report the regulation of neuronal activity in zebrafish larvae by three-photon excitation of a photoswitchable muscarinic agonist at 50 pM, a billion-fold lower concentration than used for uncaging, and with mid-infrared light of 1560 nm, the longest reported photoswitch wavelength. Robust, physiologically relevant photoresponses allow modulating brain activity in wild-type animals with spatiotemporal and pharmacological precision. Computational calculations predict that azobenzene-based ligands have high three-photon absorption cross-section and can be used directly with pulsed infrared light. The expansion of three-photon pharmacology will deeply impact basic neurobiology and neuromodulation phototherapies.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=25130
url https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=25130
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv WILEY-V C H VERLAG GMBH
publisher.none.fl_str_mv WILEY-V C H VERLAG GMBH
dc.source.none.fl_str_mv ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
ISSN: 14337851
ISSNe: 15213773
reponame:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
instname:Fundació Sant Joan de Déu
instname_str Fundació Sant Joan de Déu
reponame_str r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
collection r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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