Intermittent Hypobaric Hypoxic Preconditioning Provides Neuroprotection by Increasing Antioxidant Activity, Erythropoietin Expression and Preventing Apoptosis and Astrogliosis in the Brain of Adult Rats Exposed to Acute Severe Hypoxia

Background: Exposure to intermittent hypoxia has been demonstrated to be an efficienttool for hypoxic preconditioning, preventing damage to cells and demonstrating therapeutic benefits.We aimed to evaluate the effects of respiratory intermittent hypobaric hypoxia (IHH) to avoid braininjury caused by...

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Autores: Coimbra-Costa, Débora, Garzón, Fernando, Alva Bocanegra, Norma V. (Norma Violeta), Pinto, Tiago C. C., Aguado Tomàs, Fernando, Torrella Guio, Joan Ramon, Carbonell i Camós, Teresa, Rama Bretón, Ramón
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/179426
Acceso en línea:https://hdl.handle.net/2445/179426
Access Level:acceso abierto
Palabra clave:Apoptosi
Eritropoetina
Glutatió
Anoxèmia
Superòxid dismutasa
Apoptosis
Erythropoietin
Glutathione
Anoxemia
Superoxide dismutase
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spelling Intermittent Hypobaric Hypoxic Preconditioning Provides Neuroprotection by Increasing Antioxidant Activity, Erythropoietin Expression and Preventing Apoptosis and Astrogliosis in the Brain of Adult Rats Exposed to Acute Severe HypoxiaCoimbra-Costa, DéboraGarzón, FernandoAlva Bocanegra, Norma V. (Norma Violeta)Pinto, Tiago C. C.Aguado Tomàs, FernandoTorrella Guio, Joan RamonCarbonell i Camós, TeresaRama Bretón, RamónApoptosiEritropoetinaGlutatióAnoxèmiaSuperòxid dismutasaApoptosisErythropoietinGlutathioneAnoxemiaSuperoxide dismutaseBackground: Exposure to intermittent hypoxia has been demonstrated to be an efficienttool for hypoxic preconditioning, preventing damage to cells and demonstrating therapeutic benefits.We aimed to evaluate the effects of respiratory intermittent hypobaric hypoxia (IHH) to avoid braininjury caused by exposure to acute severe hypoxia (ASH). Methods: biomarkers of oxidative damage,mitochondrial apoptosis, and transcriptional factors in response to hypoxia were assessed by Westernblot and immunohistochemistry in brain tissue. Four groups of rats were used: (1) normoxic (NOR),(2) exposed to ASH (FiO27% for 6 h), (3) exposed to IHH for 3 h per day over 8 days at 460 mmHg,and (4) ASH preconditioned after IHH. Results: ASH animals underwent increased oxidative-stress-related parameters, an upregulation in apoptotic proteins and had astrocytes with phenotype formscompatible with severe diffuse reactive astrogliosis. These effects were attenuated and even preventedwhen the animals were preconditioned with IHH. These changes paralleled the inhibition of NF-κBexpression and the increase of erythropoietin (EPO) levels in the brain. Conclusions: IHH exertedneuroprotection against ASH-induced oxidative injury by preventing oxidative stress and inhibitingthe apoptotic cascade, which was associated with NF-κB downregulation and EPO upregulationMDPI2021202120212021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion16 p.application/pdfhttps://hdl.handle.net/2445/179426Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3390/ijms22105272International Journal of Molecular Sciences, 2021, vol. 22, num. 10, p. 1-16https://doi.org/10.3390/ijms22105272cc-by (c) Coimbra-Costa, D. et al., 2021https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1794262026-05-29T05:05:01Z
dc.title.none.fl_str_mv Intermittent Hypobaric Hypoxic Preconditioning Provides Neuroprotection by Increasing Antioxidant Activity, Erythropoietin Expression and Preventing Apoptosis and Astrogliosis in the Brain of Adult Rats Exposed to Acute Severe Hypoxia
title Intermittent Hypobaric Hypoxic Preconditioning Provides Neuroprotection by Increasing Antioxidant Activity, Erythropoietin Expression and Preventing Apoptosis and Astrogliosis in the Brain of Adult Rats Exposed to Acute Severe Hypoxia
spellingShingle Intermittent Hypobaric Hypoxic Preconditioning Provides Neuroprotection by Increasing Antioxidant Activity, Erythropoietin Expression and Preventing Apoptosis and Astrogliosis in the Brain of Adult Rats Exposed to Acute Severe Hypoxia
Coimbra-Costa, Débora
Apoptosi
Eritropoetina
Glutatió
Anoxèmia
Superòxid dismutasa
Apoptosis
Erythropoietin
Glutathione
Anoxemia
Superoxide dismutase
title_short Intermittent Hypobaric Hypoxic Preconditioning Provides Neuroprotection by Increasing Antioxidant Activity, Erythropoietin Expression and Preventing Apoptosis and Astrogliosis in the Brain of Adult Rats Exposed to Acute Severe Hypoxia
title_full Intermittent Hypobaric Hypoxic Preconditioning Provides Neuroprotection by Increasing Antioxidant Activity, Erythropoietin Expression and Preventing Apoptosis and Astrogliosis in the Brain of Adult Rats Exposed to Acute Severe Hypoxia
title_fullStr Intermittent Hypobaric Hypoxic Preconditioning Provides Neuroprotection by Increasing Antioxidant Activity, Erythropoietin Expression and Preventing Apoptosis and Astrogliosis in the Brain of Adult Rats Exposed to Acute Severe Hypoxia
title_full_unstemmed Intermittent Hypobaric Hypoxic Preconditioning Provides Neuroprotection by Increasing Antioxidant Activity, Erythropoietin Expression and Preventing Apoptosis and Astrogliosis in the Brain of Adult Rats Exposed to Acute Severe Hypoxia
title_sort Intermittent Hypobaric Hypoxic Preconditioning Provides Neuroprotection by Increasing Antioxidant Activity, Erythropoietin Expression and Preventing Apoptosis and Astrogliosis in the Brain of Adult Rats Exposed to Acute Severe Hypoxia
dc.creator.none.fl_str_mv Coimbra-Costa, Débora
Garzón, Fernando
Alva Bocanegra, Norma V. (Norma Violeta)
Pinto, Tiago C. C.
Aguado Tomàs, Fernando
Torrella Guio, Joan Ramon
Carbonell i Camós, Teresa
Rama Bretón, Ramón
author Coimbra-Costa, Débora
author_facet Coimbra-Costa, Débora
Garzón, Fernando
Alva Bocanegra, Norma V. (Norma Violeta)
Pinto, Tiago C. C.
Aguado Tomàs, Fernando
Torrella Guio, Joan Ramon
Carbonell i Camós, Teresa
Rama Bretón, Ramón
author_role author
author2 Garzón, Fernando
Alva Bocanegra, Norma V. (Norma Violeta)
Pinto, Tiago C. C.
Aguado Tomàs, Fernando
Torrella Guio, Joan Ramon
Carbonell i Camós, Teresa
Rama Bretón, Ramón
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Apoptosi
Eritropoetina
Glutatió
Anoxèmia
Superòxid dismutasa
Apoptosis
Erythropoietin
Glutathione
Anoxemia
Superoxide dismutase
topic Apoptosi
Eritropoetina
Glutatió
Anoxèmia
Superòxid dismutasa
Apoptosis
Erythropoietin
Glutathione
Anoxemia
Superoxide dismutase
description Background: Exposure to intermittent hypoxia has been demonstrated to be an efficienttool for hypoxic preconditioning, preventing damage to cells and demonstrating therapeutic benefits.We aimed to evaluate the effects of respiratory intermittent hypobaric hypoxia (IHH) to avoid braininjury caused by exposure to acute severe hypoxia (ASH). Methods: biomarkers of oxidative damage,mitochondrial apoptosis, and transcriptional factors in response to hypoxia were assessed by Westernblot and immunohistochemistry in brain tissue. Four groups of rats were used: (1) normoxic (NOR),(2) exposed to ASH (FiO27% for 6 h), (3) exposed to IHH for 3 h per day over 8 days at 460 mmHg,and (4) ASH preconditioned after IHH. Results: ASH animals underwent increased oxidative-stress-related parameters, an upregulation in apoptotic proteins and had astrocytes with phenotype formscompatible with severe diffuse reactive astrogliosis. These effects were attenuated and even preventedwhen the animals were preconditioned with IHH. These changes paralleled the inhibition of NF-κBexpression and the increase of erythropoietin (EPO) levels in the brain. Conclusions: IHH exertedneuroprotection against ASH-induced oxidative injury by preventing oxidative stress and inhibitingthe apoptotic cascade, which was associated with NF-κB downregulation and EPO upregulation
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/179426
url https://hdl.handle.net/2445/179426
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/ijms22105272
International Journal of Molecular Sciences, 2021, vol. 22, num. 10, p. 1-16
https://doi.org/10.3390/ijms22105272
dc.rights.none.fl_str_mv cc-by (c) Coimbra-Costa, D. et al., 2021
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Coimbra-Costa, D. et al., 2021
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 16 p.
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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