The DNA load of six high-risk human papillomavirus types and its association with cervical lesions

Background: Analysing human papillomavirus (HPV) viral load is important in determining the risk of developing cervical cancer (CC); most knowledge to date regarding HPV viral load and cervical lesions has been related to HPV-16. This study evaluated the association between the viral load of the six...

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Autores: Río Ospina, Luisa del, Soto de León, Sara, Camargo, Milena, Moreno Pérez, Darwin Andrés, Sánchez, Ricardo, Pérez Prados, Antonio, Patarroyo, Manuel Elkin, Patarroyo, Manuel Alfonso
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Universidad Pública de Navarra
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/18322
Acceso en línea:https://hdl.handle.net/2454/18322
Access Level:acceso abierto
Palabra clave:Cervical intraepithelial neoplasia
HR-HPV
HPV DNA load
RT-PCR
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spelling The DNA load of six high-risk human papillomavirus types and its association with cervical lesionsRío Ospina, Luisa delSoto de León, SaraCamargo, MilenaMoreno Pérez, Darwin AndrésSánchez, RicardoPérez Prados, AntonioPatarroyo, Manuel ElkinPatarroyo, Manuel AlfonsoCervical intraepithelial neoplasiaHR-HPVHPV DNA loadRT-PCRBackground: Analysing human papillomavirus (HPV) viral load is important in determining the risk of developing cervical cancer (CC); most knowledge to date regarding HPV viral load and cervical lesions has been related to HPV-16. This study evaluated the association between the viral load of the six most prevalent high-risk viral types in Colombia and cervical intraepithelial neoplasia (CIN) frequency. Methods: 114 women without CIN and 59 women having CIN confirmed by colposcopy, all of them positive by conventional PCR for HPV infection in the initial screening, were included in the study. Samples were tested for six high-risk HPV types to determine viral copy number by real-time PCR. Crude and adjusted odds ratios (ORa) were estimated for evaluating the association between each viral type's DNA load and the risk of cervical lesions occurring. Results: The highest viral loads were identified for HPV-33 in CIN patients and for HPV-31 in patients without lesions (9.33 HPV copies, 2.95 interquartile range (IQR); 9.41 HPV copies, 2.58 IQR). Lesions were more frequent in HPV-16 patients having a low viral load (3.53 ORa, 1.16-10.74 95%CI) compared to those having high HPV-16 load (2.62 ORa, 1.08-6.35 95%CI). High viral load in HPV-31 patients was associated with lower CIN frequency (0.34 ORa, 0.15-0.78 95%CI). Conclusions: An association between HPV DNA load and CIN frequency was seen to be type-specific and may have depended on the duration of infection. This analysis has provided information for understanding the effect of HPV DNA load on cervical lesion development.This project was supported by the Basque Development Cooperation Agency, the Spanish International Development Cooperation Agency (AECID) (Project 10-CAP1-0197) and the Colombian Science, Technology and Innovation Department (COLCIENCIAS) (contract # 0709-2013).BioMed CentralEstadística e Investigación OperativaEstatistika eta Ikerketa Operatiboa2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2454/18322reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglés© 2015 Del Río-Ospina et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise statedhttp://creativecommons.org/licenses/by/4.0/http://creativecommons.org/publicdomain/zero/1.0/info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/183222026-06-17T12:41:47Z
dc.title.none.fl_str_mv The DNA load of six high-risk human papillomavirus types and its association with cervical lesions
title The DNA load of six high-risk human papillomavirus types and its association with cervical lesions
spellingShingle The DNA load of six high-risk human papillomavirus types and its association with cervical lesions
Río Ospina, Luisa del
Cervical intraepithelial neoplasia
HR-HPV
HPV DNA load
RT-PCR
title_short The DNA load of six high-risk human papillomavirus types and its association with cervical lesions
title_full The DNA load of six high-risk human papillomavirus types and its association with cervical lesions
title_fullStr The DNA load of six high-risk human papillomavirus types and its association with cervical lesions
title_full_unstemmed The DNA load of six high-risk human papillomavirus types and its association with cervical lesions
title_sort The DNA load of six high-risk human papillomavirus types and its association with cervical lesions
dc.creator.none.fl_str_mv Río Ospina, Luisa del
Soto de León, Sara
Camargo, Milena
Moreno Pérez, Darwin Andrés
Sánchez, Ricardo
Pérez Prados, Antonio
Patarroyo, Manuel Elkin
Patarroyo, Manuel Alfonso
author Río Ospina, Luisa del
author_facet Río Ospina, Luisa del
Soto de León, Sara
Camargo, Milena
Moreno Pérez, Darwin Andrés
Sánchez, Ricardo
Pérez Prados, Antonio
Patarroyo, Manuel Elkin
Patarroyo, Manuel Alfonso
author_role author
author2 Soto de León, Sara
Camargo, Milena
Moreno Pérez, Darwin Andrés
Sánchez, Ricardo
Pérez Prados, Antonio
Patarroyo, Manuel Elkin
Patarroyo, Manuel Alfonso
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Estadística e Investigación Operativa
Estatistika eta Ikerketa Operatiboa
dc.subject.none.fl_str_mv Cervical intraepithelial neoplasia
HR-HPV
HPV DNA load
RT-PCR
topic Cervical intraepithelial neoplasia
HR-HPV
HPV DNA load
RT-PCR
description Background: Analysing human papillomavirus (HPV) viral load is important in determining the risk of developing cervical cancer (CC); most knowledge to date regarding HPV viral load and cervical lesions has been related to HPV-16. This study evaluated the association between the viral load of the six most prevalent high-risk viral types in Colombia and cervical intraepithelial neoplasia (CIN) frequency. Methods: 114 women without CIN and 59 women having CIN confirmed by colposcopy, all of them positive by conventional PCR for HPV infection in the initial screening, were included in the study. Samples were tested for six high-risk HPV types to determine viral copy number by real-time PCR. Crude and adjusted odds ratios (ORa) were estimated for evaluating the association between each viral type's DNA load and the risk of cervical lesions occurring. Results: The highest viral loads were identified for HPV-33 in CIN patients and for HPV-31 in patients without lesions (9.33 HPV copies, 2.95 interquartile range (IQR); 9.41 HPV copies, 2.58 IQR). Lesions were more frequent in HPV-16 patients having a low viral load (3.53 ORa, 1.16-10.74 95%CI) compared to those having high HPV-16 load (2.62 ORa, 1.08-6.35 95%CI). High viral load in HPV-31 patients was associated with lower CIN frequency (0.34 ORa, 0.15-0.78 95%CI). Conclusions: An association between HPV DNA load and CIN frequency was seen to be type-specific and may have depended on the duration of infection. This analysis has provided information for understanding the effect of HPV DNA load on cervical lesion development.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/18322
url https://hdl.handle.net/2454/18322
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
http://creativecommons.org/publicdomain/zero/1.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
http://creativecommons.org/publicdomain/zero/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
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repository.mail.fl_str_mv
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