Transforming growth factor beta (TGFβ) pathway is essential for hypoblast and epiblast development in ovine post-hatching embryos

9 Pág.

Detalhes bibliográficos
Autores: Galiano-Cogolludo, Beatriz, Marigorta, Pilar, Yus Girón, Víctor, Bermejo Álvarez, Pablo, Ramos Ibeas, Priscila
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/308360
Acesso em linha:http://hdl.handle.net/10261/308360
https://api.elsevier.com/content/abstract/scopus_id/85142454200
Access Level:acceso abierto
Palavra-chave:Embryo
Ovine
Post-blastocyst in vitro culture
ROCK inhibitor
TGFβ
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spelling Transforming growth factor beta (TGFβ) pathway is essential for hypoblast and epiblast development in ovine post-hatching embryosGaliano-Cogolludo, BeatrizMarigorta, PilarYus Girón, VíctorBermejo Álvarez, PabloRamos Ibeas, PriscilaEmbryoOvinePost-blastocyst in vitro cultureROCK inhibitorTGFβ9 Pág.The developmental failures occurring between blastocyst hatching and implantation in farm ungulates are a major cause of pregnancy losses. At the expanded blastocyst stage, three cell lineages emerge in the embryo: trophoblast, hypoblast and epiblast, the latter being the most vulnerable during post-hatching development. Transforming growth factor beta (TGFβ) signaling pathway is involved in hypoblast and epiblast development; however, previous in vitro functional studies are limited to the expanded blastocyst stage. In this study, we have analyzed the effect of TGFβ inhibition with 10, 20 or 40 μM SB431542 during ovine post-hatching developmental period using a recently developed culture system able to recapitulate major developmental landmarks. We have found a negative effect of TGFβ inhibition on hypoblast and epiblast development that could be partially reverted by Rho-associated protein kinase (ROCK) inhibitor Y-27632. Our findings provide new insights into the molecular networks regulating embryo development beyond the expanded blastocyst and could help to elucidate the causes of early pregnancy losses in farm ungulates.This work has been funded by the projects StG-757886-ELONGAN from the European Research Council to PBA and PID2021-122153NA-I00 from the Spanish Ministry of Science and Innovation to PRI.Peer reviewedElsevierEuropean CommissionMinisterio de Ciencia e Innovación (España)Galiano-Cogolludo, Beatriz [0000-0003-4687-8583]Marigorta, Pilar [0000-0002-6287-5780]Bermejo Álvarez, Pablo [0000-0001-9907-2626]Ramos Ibeas, Priscila [0000-0003-4578-0189]Bermejo Álvarez, Pablo [0000-0001-9907-2626]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/308360https://api.elsevier.com/content/abstract/scopus_id/85142454200reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/757886info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-122153NA-I00Departamento de Reproducción Animal​ https://doi.org/10.1016/j.theriogenology.2022.11.021Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3083602026-05-22T06:33:51Z
dc.title.none.fl_str_mv Transforming growth factor beta (TGFβ) pathway is essential for hypoblast and epiblast development in ovine post-hatching embryos
title Transforming growth factor beta (TGFβ) pathway is essential for hypoblast and epiblast development in ovine post-hatching embryos
spellingShingle Transforming growth factor beta (TGFβ) pathway is essential for hypoblast and epiblast development in ovine post-hatching embryos
Galiano-Cogolludo, Beatriz
Embryo
Ovine
Post-blastocyst in vitro culture
ROCK inhibitor
TGFβ
title_short Transforming growth factor beta (TGFβ) pathway is essential for hypoblast and epiblast development in ovine post-hatching embryos
title_full Transforming growth factor beta (TGFβ) pathway is essential for hypoblast and epiblast development in ovine post-hatching embryos
title_fullStr Transforming growth factor beta (TGFβ) pathway is essential for hypoblast and epiblast development in ovine post-hatching embryos
title_full_unstemmed Transforming growth factor beta (TGFβ) pathway is essential for hypoblast and epiblast development in ovine post-hatching embryos
title_sort Transforming growth factor beta (TGFβ) pathway is essential for hypoblast and epiblast development in ovine post-hatching embryos
dc.creator.none.fl_str_mv Galiano-Cogolludo, Beatriz
Marigorta, Pilar
Yus Girón, Víctor
Bermejo Álvarez, Pablo
Ramos Ibeas, Priscila
author Galiano-Cogolludo, Beatriz
author_facet Galiano-Cogolludo, Beatriz
Marigorta, Pilar
Yus Girón, Víctor
Bermejo Álvarez, Pablo
Ramos Ibeas, Priscila
author_role author
author2 Marigorta, Pilar
Yus Girón, Víctor
Bermejo Álvarez, Pablo
Ramos Ibeas, Priscila
author2_role author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Ministerio de Ciencia e Innovación (España)
Galiano-Cogolludo, Beatriz [0000-0003-4687-8583]
Marigorta, Pilar [0000-0002-6287-5780]
Bermejo Álvarez, Pablo [0000-0001-9907-2626]
Ramos Ibeas, Priscila [0000-0003-4578-0189]
Bermejo Álvarez, Pablo [0000-0001-9907-2626]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Embryo
Ovine
Post-blastocyst in vitro culture
ROCK inhibitor
TGFβ
topic Embryo
Ovine
Post-blastocyst in vitro culture
ROCK inhibitor
TGFβ
description 9 Pág.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/308360
https://api.elsevier.com/content/abstract/scopus_id/85142454200
url http://hdl.handle.net/10261/308360
https://api.elsevier.com/content/abstract/scopus_id/85142454200
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/757886
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-122153NA-I00
Departamento de Reproducción Animal​ 
https://doi.org/10.1016/j.theriogenology.2022.11.021
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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