Identification of zoonotic genotypes of Giardia duodenalis

Giardia duodenalis, originally regarded as a commensal organism, is the etiologic agent of giardiasis, a gastrointestinal disease of humans and animals. Giardiasis causes major public and veterinary health concerns worldwide. Transmission is either direct, through the faecal-oral route, or indirect,...

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Autores: Sprong, Hein, Cacciò, Simone M, van der Giessen, Joke W B, ZOOPNET network and partners, Rubio Muñoz, Jose Miguel, Fuentes Corripio, Isabel
Tipo de recurso: artículo
Fecha de publicación:2009
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/16292
Acceso en línea:http://hdl.handle.net/20.500.12105/16292
Access Level:acceso abierto
Palabra clave:Animals
Cats
Cattle
DNA, Protozoan
Dogs
Genetic Variation
Genotype
Giardia lamblia
Giardiasis
Goats
Humans
Molecular Sequence Data
Phylogeny
Sheep
Swine
Zoonoses
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spelling Identification of zoonotic genotypes of Giardia duodenalisSprong, HeinCacciò, Simone Mvan der Giessen, Joke W BZOOPNET network and partnersRubio Muñoz, Jose MiguelFuentes Corripio, IsabelAnimalsCatsCattleDNA, ProtozoanDogsGenetic VariationGenotypeGiardia lambliaGiardiasisGoatsHumansMolecular Sequence DataPhylogenySheepSwineZoonosesGiardia duodenalis, originally regarded as a commensal organism, is the etiologic agent of giardiasis, a gastrointestinal disease of humans and animals. Giardiasis causes major public and veterinary health concerns worldwide. Transmission is either direct, through the faecal-oral route, or indirect, through ingestion of contaminated water or food. Genetic characterization of G. duodenalis isolates has revealed the existence of seven groups (assemblages A to G) which differ in their host distribution. Assemblages A and B are found in humans and in many other mammals, but the role of animals in the epidemiology of human infection is still unclear, despite the fact that the zoonotic potential of Giardia was recognised by the WHO some 30 years ago. Here, we performed an extensive genetic characterization of 978 human and 1440 animal isolates, which together comprise 3886 sequences from 4 genetic loci. The data were assembled into a molecular epidemiological database developed by a European network of public and veterinary health Institutions. Genotyping was performed at different levels of resolution (single and multiple loci on the same dataset). The zoonotic potential of both assemblages A and B is evident when studied at the level of assemblages, sub-assemblages, and even at each single locus. However, when genotypes are defined using a multi-locus sequence typing scheme, only 2 multi-locus genotypes (MLG) of assemblage A and none of assemblage B appear to have a zoonotic potential. Surprisingly, mixtures of genotypes in individual isolates were repeatedly observed. Possible explanations are the uptake of genetically different Giardia cysts by a host, or subsequent infection of an already infected host, likely without overt symptoms, with a different Giardia species, which may cause disease. Other explanations for mixed genotypes, particularly for assemblage B, are substantial allelic sequence heterogeneity and/or genetic recombination. Although the zoonotic potential of G. duodenalis is evident, evidence on the contribution and frequency is (still) lacking. This newly developed molecular database has the potential to tackle intricate epidemiological questions concerning protozoan diseases.Netherlands Food and Consumer Product Safety Authority20232023-07-1920092009-12-0120092009-12-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/16292reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/162922026-06-12T12:43:37Z
dc.title.none.fl_str_mv Identification of zoonotic genotypes of Giardia duodenalis
title Identification of zoonotic genotypes of Giardia duodenalis
spellingShingle Identification of zoonotic genotypes of Giardia duodenalis
Sprong, Hein
Animals
Cats
Cattle
DNA, Protozoan
Dogs
Genetic Variation
Genotype
Giardia lamblia
Giardiasis
Goats
Humans
Molecular Sequence Data
Phylogeny
Sheep
Swine
Zoonoses
title_short Identification of zoonotic genotypes of Giardia duodenalis
title_full Identification of zoonotic genotypes of Giardia duodenalis
title_fullStr Identification of zoonotic genotypes of Giardia duodenalis
title_full_unstemmed Identification of zoonotic genotypes of Giardia duodenalis
title_sort Identification of zoonotic genotypes of Giardia duodenalis
dc.creator.none.fl_str_mv Sprong, Hein
Cacciò, Simone M
van der Giessen, Joke W B
ZOOPNET network and partners
Rubio Muñoz, Jose Miguel
Fuentes Corripio, Isabel
author Sprong, Hein
author_facet Sprong, Hein
Cacciò, Simone M
van der Giessen, Joke W B
ZOOPNET network and partners
Rubio Muñoz, Jose Miguel
Fuentes Corripio, Isabel
author_role author
author2 Cacciò, Simone M
van der Giessen, Joke W B
ZOOPNET network and partners
Rubio Muñoz, Jose Miguel
Fuentes Corripio, Isabel
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Netherlands Food and Consumer Product Safety Authority
dc.subject.none.fl_str_mv Animals
Cats
Cattle
DNA, Protozoan
Dogs
Genetic Variation
Genotype
Giardia lamblia
Giardiasis
Goats
Humans
Molecular Sequence Data
Phylogeny
Sheep
Swine
Zoonoses
topic Animals
Cats
Cattle
DNA, Protozoan
Dogs
Genetic Variation
Genotype
Giardia lamblia
Giardiasis
Goats
Humans
Molecular Sequence Data
Phylogeny
Sheep
Swine
Zoonoses
description Giardia duodenalis, originally regarded as a commensal organism, is the etiologic agent of giardiasis, a gastrointestinal disease of humans and animals. Giardiasis causes major public and veterinary health concerns worldwide. Transmission is either direct, through the faecal-oral route, or indirect, through ingestion of contaminated water or food. Genetic characterization of G. duodenalis isolates has revealed the existence of seven groups (assemblages A to G) which differ in their host distribution. Assemblages A and B are found in humans and in many other mammals, but the role of animals in the epidemiology of human infection is still unclear, despite the fact that the zoonotic potential of Giardia was recognised by the WHO some 30 years ago. Here, we performed an extensive genetic characterization of 978 human and 1440 animal isolates, which together comprise 3886 sequences from 4 genetic loci. The data were assembled into a molecular epidemiological database developed by a European network of public and veterinary health Institutions. Genotyping was performed at different levels of resolution (single and multiple loci on the same dataset). The zoonotic potential of both assemblages A and B is evident when studied at the level of assemblages, sub-assemblages, and even at each single locus. However, when genotypes are defined using a multi-locus sequence typing scheme, only 2 multi-locus genotypes (MLG) of assemblage A and none of assemblage B appear to have a zoonotic potential. Surprisingly, mixtures of genotypes in individual isolates were repeatedly observed. Possible explanations are the uptake of genetically different Giardia cysts by a host, or subsequent infection of an already infected host, likely without overt symptoms, with a different Giardia species, which may cause disease. Other explanations for mixed genotypes, particularly for assemblage B, are substantial allelic sequence heterogeneity and/or genetic recombination. Although the zoonotic potential of G. duodenalis is evident, evidence on the contribution and frequency is (still) lacking. This newly developed molecular database has the potential to tackle intricate epidemiological questions concerning protozoan diseases.
publishDate 2009
dc.date.none.fl_str_mv 2009
2009-12-01
2009
2009-12-01
2023
2023-07-19
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/16292
url http://hdl.handle.net/20.500.12105/16292
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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