Differentially Deregulated MicroRNAs as Novel Biomarkers for Neoplastic Progression in Ulcerative Colitis

Introduction: Colorectal cancer (CRC) is a potentially life-threatening complication of long-standing ulcerative colitis (UC). MicroRNAs (miRNA) are epigenetic regulators that have been involved in the development of UC-associated CRC. However, their role as potential mucosal biomarkers of neoplasti...

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Autores: Quintanilla Leo, Isabel, Jung, Gerhard, Jimeno, Mireya, Lozano Salvatella, Juan José, Sidorova, Julia, Camps, Jordi, Carballal, Sabela, Bujanda, Luis, Vera, María Isabel, Quintero, Enrique, Carrillo Palau, Marta, Cuatrecasas Freixas, Miriam, Castells Garangou, Antoni, Panés Díaz, Julià, Ricart, Elena, Moreira Ruiz, Leticia, Balaguer Prunés, Francesc, Pellisé Urquiza, Maria
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/209262
Acceso en línea:https://hdl.handle.net/2445/209262
Access Level:acceso abierto
Palabra clave:Colitis ulcerosa
Càncer colorectal
Micro RNAs
Ulcerative colitis
Colorectal cancer
MicroRNAs
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spelling Differentially Deregulated MicroRNAs as Novel Biomarkers for Neoplastic Progression in Ulcerative ColitisQuintanilla Leo, IsabelJung, GerhardJimeno, MireyaLozano Salvatella, Juan JoséSidorova, JuliaCamps, JordiCarballal, SabelaBujanda, LuisVera, María IsabelQuintero, EnriqueCarrillo Palau, MartaCuatrecasas Freixas, MiriamCastells Garangou, AntoniPanés Díaz, JuliàRicart, ElenaMoreira Ruiz, LeticiaBalaguer Prunés, FrancescPellisé Urquiza, MariaColitis ulcerosaCàncer colorectalMicro RNAsUlcerative colitisColorectal cancerMicroRNAsIntroduction: Colorectal cancer (CRC) is a potentially life-threatening complication of long-standing ulcerative colitis (UC). MicroRNAs (miRNA) are epigenetic regulators that have been involved in the development of UC-associated CRC. However, their role as potential mucosal biomarkers of neoplastic progression has not been adequately studied. Methods: In this study, we analyzed the expression of 96 preselected miRNAs in human formalin-fixed and paraffin-embedded tissue of 52 case biopsies (20 normal mucosa, 20 dysplasia, and 12 UC-associated CRCs) and 50 control biopsies (10 normal mucosa, 21 sporadic adenomas, and 19 sporadic CRCs) by using Custom TaqMan Array Cards. For validation of deregulated miRNAs, we performed individual quantitative real-time polymerase chain reaction in an independent cohort of 50 cases (13 normal mucosa, 25 dysplasia, and 12 UC-associated CRCs) and 46 controls (7 normal mucosa, 19 sporadic adenomas, and 20 sporadic CRCs). Results: Sixty-four miRNAs were found to be differentially deregulated in the UC-associated CRC sequence. Eight of these miRNAs were chosen for further validation. We confirmed miR-31, -106a, and -135b to be significantly deregulated between normal mucosa and dysplasia, as well as across the UC-associated CRC sequence (all P < 0.01). Notably, these miRNAs also confirmed to have a significant differential expression compared with sporadic CRC (all P < 0.05). Discussion: UC-associated and sporadic CRCs have distinct miRNA expression patterns, and some miRNAs indicate early neoplastic progression.Wolters Kluwer Health2024202420222024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion11 p.application/pdfhttps://hdl.handle.net/2445/209262Articles publicats en revistes (Medicina)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.14309/ctg.0000000000000489Clinical and Translational Gastroenterology, 2022, vol. 13, num.7https://doi.org/10.14309/ctg.0000000000000489cc-by-nc-nd (c) Quintanilla Leo, Isabel et al., 2022http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2092622026-05-29T05:05:01Z
dc.title.none.fl_str_mv Differentially Deregulated MicroRNAs as Novel Biomarkers for Neoplastic Progression in Ulcerative Colitis
title Differentially Deregulated MicroRNAs as Novel Biomarkers for Neoplastic Progression in Ulcerative Colitis
spellingShingle Differentially Deregulated MicroRNAs as Novel Biomarkers for Neoplastic Progression in Ulcerative Colitis
Quintanilla Leo, Isabel
Colitis ulcerosa
Càncer colorectal
Micro RNAs
Ulcerative colitis
Colorectal cancer
MicroRNAs
title_short Differentially Deregulated MicroRNAs as Novel Biomarkers for Neoplastic Progression in Ulcerative Colitis
title_full Differentially Deregulated MicroRNAs as Novel Biomarkers for Neoplastic Progression in Ulcerative Colitis
title_fullStr Differentially Deregulated MicroRNAs as Novel Biomarkers for Neoplastic Progression in Ulcerative Colitis
title_full_unstemmed Differentially Deregulated MicroRNAs as Novel Biomarkers for Neoplastic Progression in Ulcerative Colitis
title_sort Differentially Deregulated MicroRNAs as Novel Biomarkers for Neoplastic Progression in Ulcerative Colitis
dc.creator.none.fl_str_mv Quintanilla Leo, Isabel
Jung, Gerhard
Jimeno, Mireya
Lozano Salvatella, Juan José
Sidorova, Julia
Camps, Jordi
Carballal, Sabela
Bujanda, Luis
Vera, María Isabel
Quintero, Enrique
Carrillo Palau, Marta
Cuatrecasas Freixas, Miriam
Castells Garangou, Antoni
Panés Díaz, Julià
Ricart, Elena
Moreira Ruiz, Leticia
Balaguer Prunés, Francesc
Pellisé Urquiza, Maria
author Quintanilla Leo, Isabel
author_facet Quintanilla Leo, Isabel
Jung, Gerhard
Jimeno, Mireya
Lozano Salvatella, Juan José
Sidorova, Julia
Camps, Jordi
Carballal, Sabela
Bujanda, Luis
Vera, María Isabel
Quintero, Enrique
Carrillo Palau, Marta
Cuatrecasas Freixas, Miriam
Castells Garangou, Antoni
Panés Díaz, Julià
Ricart, Elena
Moreira Ruiz, Leticia
Balaguer Prunés, Francesc
Pellisé Urquiza, Maria
author_role author
author2 Jung, Gerhard
Jimeno, Mireya
Lozano Salvatella, Juan José
Sidorova, Julia
Camps, Jordi
Carballal, Sabela
Bujanda, Luis
Vera, María Isabel
Quintero, Enrique
Carrillo Palau, Marta
Cuatrecasas Freixas, Miriam
Castells Garangou, Antoni
Panés Díaz, Julià
Ricart, Elena
Moreira Ruiz, Leticia
Balaguer Prunés, Francesc
Pellisé Urquiza, Maria
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Colitis ulcerosa
Càncer colorectal
Micro RNAs
Ulcerative colitis
Colorectal cancer
MicroRNAs
topic Colitis ulcerosa
Càncer colorectal
Micro RNAs
Ulcerative colitis
Colorectal cancer
MicroRNAs
description Introduction: Colorectal cancer (CRC) is a potentially life-threatening complication of long-standing ulcerative colitis (UC). MicroRNAs (miRNA) are epigenetic regulators that have been involved in the development of UC-associated CRC. However, their role as potential mucosal biomarkers of neoplastic progression has not been adequately studied. Methods: In this study, we analyzed the expression of 96 preselected miRNAs in human formalin-fixed and paraffin-embedded tissue of 52 case biopsies (20 normal mucosa, 20 dysplasia, and 12 UC-associated CRCs) and 50 control biopsies (10 normal mucosa, 21 sporadic adenomas, and 19 sporadic CRCs) by using Custom TaqMan Array Cards. For validation of deregulated miRNAs, we performed individual quantitative real-time polymerase chain reaction in an independent cohort of 50 cases (13 normal mucosa, 25 dysplasia, and 12 UC-associated CRCs) and 46 controls (7 normal mucosa, 19 sporadic adenomas, and 20 sporadic CRCs). Results: Sixty-four miRNAs were found to be differentially deregulated in the UC-associated CRC sequence. Eight of these miRNAs were chosen for further validation. We confirmed miR-31, -106a, and -135b to be significantly deregulated between normal mucosa and dysplasia, as well as across the UC-associated CRC sequence (all P < 0.01). Notably, these miRNAs also confirmed to have a significant differential expression compared with sporadic CRC (all P < 0.05). Discussion: UC-associated and sporadic CRCs have distinct miRNA expression patterns, and some miRNAs indicate early neoplastic progression.
publishDate 2022
dc.date.none.fl_str_mv 2022
2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/209262
url https://hdl.handle.net/2445/209262
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.14309/ctg.0000000000000489
Clinical and Translational Gastroenterology, 2022, vol. 13, num.7
https://doi.org/10.14309/ctg.0000000000000489
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Quintanilla Leo, Isabel et al., 2022
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Quintanilla Leo, Isabel et al., 2022
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11 p.
application/pdf
dc.publisher.none.fl_str_mv Wolters Kluwer Health
publisher.none.fl_str_mv Wolters Kluwer Health
dc.source.none.fl_str_mv Articles publicats en revistes (Medicina)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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