The follicular fluid regulates sperm capacitation and the acrosome reaction in the donkey while alleviating oxidative stress

The follicular fluid (FF) is a complex and dynamic biological medium that surrounds the oocytes during their development and growth. This fluid provides the necessary nutrients to both the oocyte and the surrounding cells, enabling the proper maturation of the oocyte. Additionally, the FF is involve...

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Autores: Miró, Jordi|||0000-0002-1085-0039, Catalán, Jaime|||0000-0002-0878-7819, Padilla, Lorena, Maside, Carolina|||0000-0003-0872-9122, Martínez-Hernández, Jesús, Yeste Oliveras, Marc|||0000-0002-2209-340X
Formato: artículo
Fecha de publicación:2025
País:España
Recursos:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:321607
Acesso em linha:https://ddd.uab.cat/record/321607
https://dx.doi.org/urn:doi:10.1016/j.anireprosci.2025.108016
Access Level:acceso abierto
Palavra-chave:Donkey
Follicular fluid
Sperm function
Capacitation
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spelling The follicular fluid regulates sperm capacitation and the acrosome reaction in the donkey while alleviating oxidative stressMiró, Jordi|||0000-0002-1085-0039Catalán, Jaime|||0000-0002-0878-7819Padilla, LorenaMaside, Carolina|||0000-0003-0872-9122Martínez-Hernández, JesúsYeste Oliveras, Marc|||0000-0002-2209-340XDonkeyFollicular fluidSperm functionCapacitationThe follicular fluid (FF) is a complex and dynamic biological medium that surrounds the oocytes during their development and growth. This fluid provides the necessary nutrients to both the oocyte and the surrounding cells, enabling the proper maturation of the oocyte. Additionally, the FF is involved in key sperm function processes, such as capacitation, which occurs when sperm reach the oviduct and are exposed to this fluid. Nevertheless, how this happens in donkeys has not been investigated. For this reason, the present study aimed to evaluate the influence of the FF on sperm capacitation in the donkey. Preovulatory FF was obtained from five different Catalan jennies (two follicles per animal), and nine ejaculates from fertile Catalan donkeys were collected. Sperm were resuspended in four different treatments: non-capacitating medium (TBM), noncapacitating medium supplemented with 20 % FF (TBM+FF), capacitating medium (TCM), and capacitating medium supplemented with 20 % FF (TCM+FF). Samples were incubated at 38 ◦C for 120 min, and at 60 min, progesterone was added to induce the acrosome reaction. After 0, 60, 70, and 120 min of incubation, sperm motility, membrane lipid disorder, acrosome integrity, mitochondrial membrane potential (MMP), and intracellular concentrations of calcium, reactive oxygen species (ROS) and superoxides were evaluated. These results suggest that the preovulatory FF promotes sperm capacitation in donkeys, as it increases intracellular calcium, membrane lipid disorder, and acrosome exocytosis, while maintaining sperm motility and reducing ROS concentration. Although the results are not entirely conclusive and further tests are needed, this study supports that the FF modulates sperm capacitation and alleviates the oxidative stress associated with this process. 22025-01-0120252025-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/321607https://dx.doi.org/urn:doi:10.1016/j.anireprosci.2025.108016reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Ciencia e Innovación https://doi.org/10.13039/501100004837 JDC2022-049684-IAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2023-149853OB-I00Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-113320RB-I00Agencia de Gestió d'Ajuts Universitaris i de Recerca 2021-SGR-00900open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3216072026-06-06T12:50:31Z
dc.title.none.fl_str_mv The follicular fluid regulates sperm capacitation and the acrosome reaction in the donkey while alleviating oxidative stress
title The follicular fluid regulates sperm capacitation and the acrosome reaction in the donkey while alleviating oxidative stress
spellingShingle The follicular fluid regulates sperm capacitation and the acrosome reaction in the donkey while alleviating oxidative stress
Miró, Jordi|||0000-0002-1085-0039
Donkey
Follicular fluid
Sperm function
Capacitation
title_short The follicular fluid regulates sperm capacitation and the acrosome reaction in the donkey while alleviating oxidative stress
title_full The follicular fluid regulates sperm capacitation and the acrosome reaction in the donkey while alleviating oxidative stress
title_fullStr The follicular fluid regulates sperm capacitation and the acrosome reaction in the donkey while alleviating oxidative stress
title_full_unstemmed The follicular fluid regulates sperm capacitation and the acrosome reaction in the donkey while alleviating oxidative stress
title_sort The follicular fluid regulates sperm capacitation and the acrosome reaction in the donkey while alleviating oxidative stress
dc.creator.none.fl_str_mv Miró, Jordi|||0000-0002-1085-0039
Catalán, Jaime|||0000-0002-0878-7819
Padilla, Lorena
Maside, Carolina|||0000-0003-0872-9122
Martínez-Hernández, Jesús
Yeste Oliveras, Marc|||0000-0002-2209-340X
author Miró, Jordi|||0000-0002-1085-0039
author_facet Miró, Jordi|||0000-0002-1085-0039
Catalán, Jaime|||0000-0002-0878-7819
Padilla, Lorena
Maside, Carolina|||0000-0003-0872-9122
Martínez-Hernández, Jesús
Yeste Oliveras, Marc|||0000-0002-2209-340X
author_role author
author2 Catalán, Jaime|||0000-0002-0878-7819
Padilla, Lorena
Maside, Carolina|||0000-0003-0872-9122
Martínez-Hernández, Jesús
Yeste Oliveras, Marc|||0000-0002-2209-340X
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Donkey
Follicular fluid
Sperm function
Capacitation
topic Donkey
Follicular fluid
Sperm function
Capacitation
description The follicular fluid (FF) is a complex and dynamic biological medium that surrounds the oocytes during their development and growth. This fluid provides the necessary nutrients to both the oocyte and the surrounding cells, enabling the proper maturation of the oocyte. Additionally, the FF is involved in key sperm function processes, such as capacitation, which occurs when sperm reach the oviduct and are exposed to this fluid. Nevertheless, how this happens in donkeys has not been investigated. For this reason, the present study aimed to evaluate the influence of the FF on sperm capacitation in the donkey. Preovulatory FF was obtained from five different Catalan jennies (two follicles per animal), and nine ejaculates from fertile Catalan donkeys were collected. Sperm were resuspended in four different treatments: non-capacitating medium (TBM), noncapacitating medium supplemented with 20 % FF (TBM+FF), capacitating medium (TCM), and capacitating medium supplemented with 20 % FF (TCM+FF). Samples were incubated at 38 ◦C for 120 min, and at 60 min, progesterone was added to induce the acrosome reaction. After 0, 60, 70, and 120 min of incubation, sperm motility, membrane lipid disorder, acrosome integrity, mitochondrial membrane potential (MMP), and intracellular concentrations of calcium, reactive oxygen species (ROS) and superoxides were evaluated. These results suggest that the preovulatory FF promotes sperm capacitation in donkeys, as it increases intracellular calcium, membrane lipid disorder, and acrosome exocytosis, while maintaining sperm motility and reducing ROS concentration. Although the results are not entirely conclusive and further tests are needed, this study supports that the FF modulates sperm capacitation and alleviates the oxidative stress associated with this process.
publishDate 2025
dc.date.none.fl_str_mv 2
2025-01-01
2025
2025-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/321607
https://dx.doi.org/urn:doi:10.1016/j.anireprosci.2025.108016
url https://ddd.uab.cat/record/321607
https://dx.doi.org/urn:doi:10.1016/j.anireprosci.2025.108016
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 JDC2022-049684-I
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2023-149853OB-I00
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-113320RB-I00
Agencia de Gestió d'Ajuts Universitaris i de Recerca 2021-SGR-00900
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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