Enhanced artemisinin yield by expression of rol genes in artemisia annua

Background: Despite of many advances in the treatment of malaria, it is still the fifth most prevalent disease worldwide and is one of the major causes of death in the developing countries which accounted for 584,000 deaths in 2013, as estimated by World Health Organization. Artemisinin from Artemis...

Descripción completa

Detalles Bibliográficos
Autores: Dilshad, Erum, Cusidó Vidal, Rosa M., Palazón Barandela, Javier, Ramírez Estrada, Karla, Bonfill Baldrich, Ma. Mercedes, Mirza, Bushra
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/100901
Acceso en línea:https://hdl.handle.net/2445/100901
Access Level:acceso abierto
Palabra clave:Compostes
Malària
Compositae
Malaria
id ES_ec93dfbc6fda5e22d8d4cfdbc563ed3b
oai_identifier_str oai:recercat.cat:2445/100901
network_acronym_str ES
network_name_str España
repository_id_str
spelling Enhanced artemisinin yield by expression of rol genes in artemisia annuaDilshad, ErumCusidó Vidal, Rosa M.Palazón Barandela, JavierRamírez Estrada, KarlaBonfill Baldrich, Ma. MercedesMirza, BushraCompostesMalàriaCompositaeMalariaBackground: Despite of many advances in the treatment of malaria, it is still the fifth most prevalent disease worldwide and is one of the major causes of death in the developing countries which accounted for 584,000 deaths in 2013, as estimated by World Health Organization. Artemisinin from Artemisia annua is still one of the most effective treatments for malaria. Increasing the artemisinin content of A. annua plants by genetic engineering would improve the availability of this much-needed drug. Methods: In this regard, a high artemisinin-yielding hybrid of A. annua produced by the centre for novel agricultural products of the University of York, UK, was selected (artemisinin maximally 1.4 %). As rol genes are potential candidates of biochemical engineering, genetic transformation of A. annua with Agrobacterium tumefaciens GV3101 harbouring vectors with rol B and rol C genes was carried out with the objective of enhancement of artemisinin content. Transgenic lines produced were analysed by the LC-MS for quantitative analysis of artemisinin and analogues. These high artemisinin yielding transgenics were also analysed by real time quantitative PCR to find the molecular dynamics of artemisinin enhancement. Genes of artemisinin biosynthetic pathway were studied including amorphadiene synthase (ADS), cytochrome P450, (CYP71AV1) and aldehyde dehydrogenase 1 (ALDH1). Trichome-specific fatty acyl-CoA reductase 1(TAFR1) is an enzyme involved in both trichome development and sesquiterpenoid biosynthesis and both processes are important for artemisinin biosynthesis. Thus, real time qPCR analysis of the TAFR1 gene was carried out, and trichome density was determined. Results: Transgenics of rol B gene showed two- to ninefold (the decimal adds nothing in the abstract, please simplify to two- to ninefold) increase in artemisinin, 4-12-fold increase in artesunate and 1.2-3-fold increase in dihydroartemisinin. Whereas in the case of rol C gene transformants, a fourfold increase in artemisinin, four to ninefold increase in artesunate and one- to twofold increase in dihydroartemisinin concentration was observed. Transformants with the rol B gene had higher expression of these genes than rol C transformants. TAFR1 was also found to be more expressed in rol gene transgenics than wild type A. annua, which was also in accordance with the trichome density of the respective plant. Conclusion: Thus it was proved that rol B and rol C genes are effective in the enhancement of artemisinin content of A. annua, rol B gene being more active to play part in this enhancement than rol C gene.BioMed Central2016201620152016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion10 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/100901Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: http://dx.doi.org/10.1186/s12936-015-0951-5Malaria Journal, 2015, vol. 14, num. 424http://dx.doi.org/10.1186/s12936-015-0951-5cc-by (c) Dilshad, E. et al., 2015http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1009012026-05-29T05:05:01Z
dc.title.none.fl_str_mv Enhanced artemisinin yield by expression of rol genes in artemisia annua
title Enhanced artemisinin yield by expression of rol genes in artemisia annua
spellingShingle Enhanced artemisinin yield by expression of rol genes in artemisia annua
Dilshad, Erum
Compostes
Malària
Compositae
Malaria
title_short Enhanced artemisinin yield by expression of rol genes in artemisia annua
title_full Enhanced artemisinin yield by expression of rol genes in artemisia annua
title_fullStr Enhanced artemisinin yield by expression of rol genes in artemisia annua
title_full_unstemmed Enhanced artemisinin yield by expression of rol genes in artemisia annua
title_sort Enhanced artemisinin yield by expression of rol genes in artemisia annua
dc.creator.none.fl_str_mv Dilshad, Erum
Cusidó Vidal, Rosa M.
Palazón Barandela, Javier
Ramírez Estrada, Karla
Bonfill Baldrich, Ma. Mercedes
Mirza, Bushra
author Dilshad, Erum
author_facet Dilshad, Erum
Cusidó Vidal, Rosa M.
Palazón Barandela, Javier
Ramírez Estrada, Karla
Bonfill Baldrich, Ma. Mercedes
Mirza, Bushra
author_role author
author2 Cusidó Vidal, Rosa M.
Palazón Barandela, Javier
Ramírez Estrada, Karla
Bonfill Baldrich, Ma. Mercedes
Mirza, Bushra
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Compostes
Malària
Compositae
Malaria
topic Compostes
Malària
Compositae
Malaria
description Background: Despite of many advances in the treatment of malaria, it is still the fifth most prevalent disease worldwide and is one of the major causes of death in the developing countries which accounted for 584,000 deaths in 2013, as estimated by World Health Organization. Artemisinin from Artemisia annua is still one of the most effective treatments for malaria. Increasing the artemisinin content of A. annua plants by genetic engineering would improve the availability of this much-needed drug. Methods: In this regard, a high artemisinin-yielding hybrid of A. annua produced by the centre for novel agricultural products of the University of York, UK, was selected (artemisinin maximally 1.4 %). As rol genes are potential candidates of biochemical engineering, genetic transformation of A. annua with Agrobacterium tumefaciens GV3101 harbouring vectors with rol B and rol C genes was carried out with the objective of enhancement of artemisinin content. Transgenic lines produced were analysed by the LC-MS for quantitative analysis of artemisinin and analogues. These high artemisinin yielding transgenics were also analysed by real time quantitative PCR to find the molecular dynamics of artemisinin enhancement. Genes of artemisinin biosynthetic pathway were studied including amorphadiene synthase (ADS), cytochrome P450, (CYP71AV1) and aldehyde dehydrogenase 1 (ALDH1). Trichome-specific fatty acyl-CoA reductase 1(TAFR1) is an enzyme involved in both trichome development and sesquiterpenoid biosynthesis and both processes are important for artemisinin biosynthesis. Thus, real time qPCR analysis of the TAFR1 gene was carried out, and trichome density was determined. Results: Transgenics of rol B gene showed two- to ninefold (the decimal adds nothing in the abstract, please simplify to two- to ninefold) increase in artemisinin, 4-12-fold increase in artesunate and 1.2-3-fold increase in dihydroartemisinin. Whereas in the case of rol C gene transformants, a fourfold increase in artemisinin, four to ninefold increase in artesunate and one- to twofold increase in dihydroartemisinin concentration was observed. Transformants with the rol B gene had higher expression of these genes than rol C transformants. TAFR1 was also found to be more expressed in rol gene transgenics than wild type A. annua, which was also in accordance with the trichome density of the respective plant. Conclusion: Thus it was proved that rol B and rol C genes are effective in the enhancement of artemisinin content of A. annua, rol B gene being more active to play part in this enhancement than rol C gene.
publishDate 2015
dc.date.none.fl_str_mv 2015
2016
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/100901
url https://hdl.handle.net/2445/100901
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1186/s12936-015-0951-5
Malaria Journal, 2015, vol. 14, num. 424
http://dx.doi.org/10.1186/s12936-015-0951-5
dc.rights.none.fl_str_mv cc-by (c) Dilshad, E. et al., 2015
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Dilshad, E. et al., 2015
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869423327019270144
score 15.811543

Similares en LA Referencia