Gold half-shell-coated paclitaxel-loaded PLGA nanoparticles for the targeted chemo-photothermal treatment of cancer

Conventional cancer therapies suffer from nonspecificity, drug resistance, and a poor bioavailability, which trigger severe side effects. To overcome these disadvantages, in this study, we designed and evaluated the in vitro potential of paclitaxel-loaded, PLGA-gold, half-shell nanoparticles (PTX-PL...

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Detalles Bibliográficos
Autores: Ibarra, Jaime, Encinas Basurto, David, Almada, Mario, Juárez, Josué, Valdez, Miguel Ángel, Barbosa Fernández, Silvia, Taboada Antelo, Pablo
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad de Santiago de Compostela (USC)
Repositorio:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Idioma:inglés
OAI Identifier:oai:minerva.usc.gal:10347/43939
Acceso en línea:https://hdl.handle.net/10347/43939
Access Level:acceso abierto
Palabra clave:Paclitaxel
PLGA
Half shell
CyRGDk peptide
Chemo-photothermal therapy
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spelling Gold half-shell-coated paclitaxel-loaded PLGA nanoparticles for the targeted chemo-photothermal treatment of cancerIbarra, JaimeEncinas Basurto, DavidAlmada, MarioJuárez, JosuéValdez, Miguel ÁngelBarbosa Fernández, SilviaTaboada Antelo, PabloPaclitaxelPLGAHalf shellCyRGDk peptideChemo-photothermal therapyConventional cancer therapies suffer from nonspecificity, drug resistance, and a poor bioavailability, which trigger severe side effects. To overcome these disadvantages, in this study, we designed and evaluated the in vitro potential of paclitaxel-loaded, PLGA-gold, half-shell nanoparticles (PTX-PLGA/Au-HS NPs) conjugated with cyclo(Arg-Gly-Asp-Phe-Lys) (cyRGDfk) as a targeted chemo-photothermal therapy system in HeLa and MDA-MB-231 cancer cells. A TEM analysis confirmed the successful gold half-shell structure formation. High-performance liquid chromatography showed an encapsulation efficiency of the paclitaxel inside nanoparticles of more than 90%. In the release study, an initial burst release of about 20% in the first 24 h was observed, followed by a sustained drug release for a period as long as 10 days, reaching values of about 92% and 49% for NPs with and without near infrared laser irradiation. In in vitro cell internalization studies, targeted nanoparticles showed a higher accumulation than nontargeted nanoparticles, possibly through a specific interaction of the cyRGDfk with their homologous receptors, the ανβ3 y ανβ5 integrins on the cell surface. Compared with chemotherapy or photothermal treatment alone, the combined treatment demonstrated a synergistic effect, reducing the cell viability to 23% for the HeLa cells and 31% for the MDA-MB-231 cells. Thus, our results indicate that these multifuncional nanoparticles can be considered to be a promising targeted chemo-photothermal therapy system against cancer.MDPIUniversidade de Santiago de Compostela. Departamento de Física de PartículasUniversidade de Santiago de Compostela. Instituto de Materiais (iMATUS)20232023-07-0820232023-07-08journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10347/43939reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:minerva.usc.gal:10347/439392026-06-15T12:47:27Z
dc.title.none.fl_str_mv Gold half-shell-coated paclitaxel-loaded PLGA nanoparticles for the targeted chemo-photothermal treatment of cancer
title Gold half-shell-coated paclitaxel-loaded PLGA nanoparticles for the targeted chemo-photothermal treatment of cancer
spellingShingle Gold half-shell-coated paclitaxel-loaded PLGA nanoparticles for the targeted chemo-photothermal treatment of cancer
Ibarra, Jaime
Paclitaxel
PLGA
Half shell
CyRGDk peptide
Chemo-photothermal therapy
title_short Gold half-shell-coated paclitaxel-loaded PLGA nanoparticles for the targeted chemo-photothermal treatment of cancer
title_full Gold half-shell-coated paclitaxel-loaded PLGA nanoparticles for the targeted chemo-photothermal treatment of cancer
title_fullStr Gold half-shell-coated paclitaxel-loaded PLGA nanoparticles for the targeted chemo-photothermal treatment of cancer
title_full_unstemmed Gold half-shell-coated paclitaxel-loaded PLGA nanoparticles for the targeted chemo-photothermal treatment of cancer
title_sort Gold half-shell-coated paclitaxel-loaded PLGA nanoparticles for the targeted chemo-photothermal treatment of cancer
dc.creator.none.fl_str_mv Ibarra, Jaime
Encinas Basurto, David
Almada, Mario
Juárez, Josué
Valdez, Miguel Ángel
Barbosa Fernández, Silvia
Taboada Antelo, Pablo
author Ibarra, Jaime
author_facet Ibarra, Jaime
Encinas Basurto, David
Almada, Mario
Juárez, Josué
Valdez, Miguel Ángel
Barbosa Fernández, Silvia
Taboada Antelo, Pablo
author_role author
author2 Encinas Basurto, David
Almada, Mario
Juárez, Josué
Valdez, Miguel Ángel
Barbosa Fernández, Silvia
Taboada Antelo, Pablo
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de Santiago de Compostela. Departamento de Física de Partículas
Universidade de Santiago de Compostela. Instituto de Materiais (iMATUS)
dc.subject.none.fl_str_mv Paclitaxel
PLGA
Half shell
CyRGDk peptide
Chemo-photothermal therapy
topic Paclitaxel
PLGA
Half shell
CyRGDk peptide
Chemo-photothermal therapy
description Conventional cancer therapies suffer from nonspecificity, drug resistance, and a poor bioavailability, which trigger severe side effects. To overcome these disadvantages, in this study, we designed and evaluated the in vitro potential of paclitaxel-loaded, PLGA-gold, half-shell nanoparticles (PTX-PLGA/Au-HS NPs) conjugated with cyclo(Arg-Gly-Asp-Phe-Lys) (cyRGDfk) as a targeted chemo-photothermal therapy system in HeLa and MDA-MB-231 cancer cells. A TEM analysis confirmed the successful gold half-shell structure formation. High-performance liquid chromatography showed an encapsulation efficiency of the paclitaxel inside nanoparticles of more than 90%. In the release study, an initial burst release of about 20% in the first 24 h was observed, followed by a sustained drug release for a period as long as 10 days, reaching values of about 92% and 49% for NPs with and without near infrared laser irradiation. In in vitro cell internalization studies, targeted nanoparticles showed a higher accumulation than nontargeted nanoparticles, possibly through a specific interaction of the cyRGDfk with their homologous receptors, the ανβ3 y ανβ5 integrins on the cell surface. Compared with chemotherapy or photothermal treatment alone, the combined treatment demonstrated a synergistic effect, reducing the cell viability to 23% for the HeLa cells and 31% for the MDA-MB-231 cells. Thus, our results indicate that these multifuncional nanoparticles can be considered to be a promising targeted chemo-photothermal therapy system against cancer.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-07-08
2023
2023-07-08
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10347/43939
url https://hdl.handle.net/10347/43939
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
instname:Universidad de Santiago de Compostela (USC)
instname_str Universidad de Santiago de Compostela (USC)
reponame_str Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
collection Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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