The Transcription Factor FEZF1, a Direct Target of EWSR1-FLI1 in Ewing Sarcoma Cells, Regulates the Expression of Neural-Specific Genes

Ewing sarcoma is a rare pediatric tumor characterized by chromosomal translocations that give rise to aberrant chimeric transcription factors (e.g., EWSR1-FLI1). EWSR1-FLI1 promotes a spe cific cellular transcriptional program. Therefore, the study of EWSR1-FLI1 target genes is important to identify...

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Autores: García García, Laura, Fernández Tabanera, Enrique, Cervera, Saint T, Melero Fernandez de Mera, Raquel Maria, Josa, Santiago, González-González, Laura, Rodríguez-Martín, Carlos, Grünewald, Thomas G P, Alonso, Javier
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad Camilo José Cela (UCJC)
Repositorio:Depósito Digital e-UCJC
OAI Identifier:oai:repositorio.ucjc.edu:20.500.12020/1205
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616448/pdf/cancers-13-05668.pdf
http://hdl.handle.net/20.500.12020/1205
Access Level:acceso abierto
Palabra clave:Biología Celular y Molecular
Ciencias Biomédicas
Ewing sarcoma
EWSR1-FLI1
GGAA-microsatellites
FEZF11
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repository_id_str
spelling The Transcription Factor FEZF1, a Direct Target of EWSR1-FLI1 in Ewing Sarcoma Cells, Regulates the Expression of Neural-Specific GenesGarcía García, LauraFernández Tabanera, EnriqueCervera, Saint TMelero Fernandez de Mera, Raquel MariaJosa, SantiagoGonzález-González, LauraRodríguez-Martín, CarlosGrünewald, Thomas G PAlonso, JavierBiología Celular y MolecularCiencias BiomédicasEwing sarcomaEWSR1-FLI1GGAA-microsatellitesFEZF11Ewing sarcoma is a rare pediatric tumor characterized by chromosomal translocations that give rise to aberrant chimeric transcription factors (e.g., EWSR1-FLI1). EWSR1-FLI1 promotes a spe cific cellular transcriptional program. Therefore, the study of EWSR1-FLI1 target genes is important to identify critical pathways involved in Ewing sarcoma tumorigenesis. In this work, we focused on the transcription factors regulated by EWSR1-FLI1 in Ewing sarcoma. Transcriptomic analysis of the Ewing sarcoma cell line A673 indicated that one of the genes more strongly upregulated by EWSR1-FLI1 was FEZF1 (FEZ family zinc finger protein 1), a transcriptional repressor involved in neural cell identity. The functional characterization of FEZF1 was performed in three Ewing sarcoma cell lines (A673, SK-N-MC, SK-ES-1) through an shRNA-directed silencing approach. FEZF1 knock down inhibited clonogenicity and cell proliferation. Finally, the analysis of the FEZF1-dependent expression profile in A673 cells showed several neural genes regulated by FEZF1 and concomitantly regulated by EWSR1-FLI1. In summary, FEZF1 is transcriptionally regulated by EWSR1-FLI1 in Ewing sarcoma cells and is involved in the regulation of neural-specific genes, which could explain the neural-like phenotype observed in several Ewing sarcoma tumors and cell linesMDPI2021info:eu-repo/semantics/articlehttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616448/pdf/cancers-13-05668.pdfhttp://hdl.handle.net/20.500.12020/1205reponame:Depósito Digital e-UCJCinstname:Universidad Camilo José Cela (UCJC)Inglésinfo:eu-repo/semantics/openAccessoai:repositorio.ucjc.edu:20.500.12020/12052026-05-27T07:36:51Z
dc.title.none.fl_str_mv The Transcription Factor FEZF1, a Direct Target of EWSR1-FLI1 in Ewing Sarcoma Cells, Regulates the Expression of Neural-Specific Genes
title The Transcription Factor FEZF1, a Direct Target of EWSR1-FLI1 in Ewing Sarcoma Cells, Regulates the Expression of Neural-Specific Genes
spellingShingle The Transcription Factor FEZF1, a Direct Target of EWSR1-FLI1 in Ewing Sarcoma Cells, Regulates the Expression of Neural-Specific Genes
García García, Laura
Biología Celular y Molecular
Ciencias Biomédicas
Ewing sarcoma
EWSR1-FLI1
GGAA-microsatellites
FEZF11
title_short The Transcription Factor FEZF1, a Direct Target of EWSR1-FLI1 in Ewing Sarcoma Cells, Regulates the Expression of Neural-Specific Genes
title_full The Transcription Factor FEZF1, a Direct Target of EWSR1-FLI1 in Ewing Sarcoma Cells, Regulates the Expression of Neural-Specific Genes
title_fullStr The Transcription Factor FEZF1, a Direct Target of EWSR1-FLI1 in Ewing Sarcoma Cells, Regulates the Expression of Neural-Specific Genes
title_full_unstemmed The Transcription Factor FEZF1, a Direct Target of EWSR1-FLI1 in Ewing Sarcoma Cells, Regulates the Expression of Neural-Specific Genes
title_sort The Transcription Factor FEZF1, a Direct Target of EWSR1-FLI1 in Ewing Sarcoma Cells, Regulates the Expression of Neural-Specific Genes
dc.creator.none.fl_str_mv García García, Laura
Fernández Tabanera, Enrique
Cervera, Saint T
Melero Fernandez de Mera, Raquel Maria
Josa, Santiago
González-González, Laura
Rodríguez-Martín, Carlos
Grünewald, Thomas G P
Alonso, Javier
author García García, Laura
author_facet García García, Laura
Fernández Tabanera, Enrique
Cervera, Saint T
Melero Fernandez de Mera, Raquel Maria
Josa, Santiago
González-González, Laura
Rodríguez-Martín, Carlos
Grünewald, Thomas G P
Alonso, Javier
author_role author
author2 Fernández Tabanera, Enrique
Cervera, Saint T
Melero Fernandez de Mera, Raquel Maria
Josa, Santiago
González-González, Laura
Rodríguez-Martín, Carlos
Grünewald, Thomas G P
Alonso, Javier
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología Celular y Molecular
Ciencias Biomédicas
Ewing sarcoma
EWSR1-FLI1
GGAA-microsatellites
FEZF11
topic Biología Celular y Molecular
Ciencias Biomédicas
Ewing sarcoma
EWSR1-FLI1
GGAA-microsatellites
FEZF11
description Ewing sarcoma is a rare pediatric tumor characterized by chromosomal translocations that give rise to aberrant chimeric transcription factors (e.g., EWSR1-FLI1). EWSR1-FLI1 promotes a spe cific cellular transcriptional program. Therefore, the study of EWSR1-FLI1 target genes is important to identify critical pathways involved in Ewing sarcoma tumorigenesis. In this work, we focused on the transcription factors regulated by EWSR1-FLI1 in Ewing sarcoma. Transcriptomic analysis of the Ewing sarcoma cell line A673 indicated that one of the genes more strongly upregulated by EWSR1-FLI1 was FEZF1 (FEZ family zinc finger protein 1), a transcriptional repressor involved in neural cell identity. The functional characterization of FEZF1 was performed in three Ewing sarcoma cell lines (A673, SK-N-MC, SK-ES-1) through an shRNA-directed silencing approach. FEZF1 knock down inhibited clonogenicity and cell proliferation. Finally, the analysis of the FEZF1-dependent expression profile in A673 cells showed several neural genes regulated by FEZF1 and concomitantly regulated by EWSR1-FLI1. In summary, FEZF1 is transcriptionally regulated by EWSR1-FLI1 in Ewing sarcoma cells and is involved in the regulation of neural-specific genes, which could explain the neural-like phenotype observed in several Ewing sarcoma tumors and cell lines
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616448/pdf/cancers-13-05668.pdf
http://hdl.handle.net/20.500.12020/1205
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616448/pdf/cancers-13-05668.pdf
http://hdl.handle.net/20.500.12020/1205
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Depósito Digital e-UCJC
instname:Universidad Camilo José Cela (UCJC)
instname_str Universidad Camilo José Cela (UCJC)
reponame_str Depósito Digital e-UCJC
collection Depósito Digital e-UCJC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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