A vaccinomics approach for the identification of tick protective antigens for the control of Ixodes ricinus and Dermacentor reticulatus infestations in companion animals

Ticks and tick-borne pathogens affect health and welfare of companion animals worldwide, and some human tick-borne diseases are associated with exposure to domestic animals. Vaccines are the most environmentally friendly alternative to acaracides for the control of tick infestations, and to reduce t...

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Detalles Bibliográficos
Autores: Contreras, Marinela, Villar, Margarita, Fuente, José de la
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/215380
Acceso en línea:http://hdl.handle.net/10261/215380
Access Level:acceso abierto
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spelling A vaccinomics approach for the identification of tick protective antigens for the control of Ixodes ricinus and Dermacentor reticulatus infestations in companion animalsContreras, MarinelaVillar, MargaritaFuente, José de laTicks and tick-borne pathogens affect health and welfare of companion animals worldwide, and some human tick-borne diseases are associated with exposure to domestic animals. Vaccines are the most environmentally friendly alternative to acaracides for the control of tick infestations, and to reduce the risk for tick-borne diseases affecting human and animal health. However, vaccines have not been developed or successfully implemented for most vector-borne diseases. The main limitation for the development of effective vaccines is the identification of protective antigens. To address this limitation, in this study we used an experimental approach combining vaccinomics based on transcriptomics and proteomics data with vaccination trials for the identification of tick protective antigens. The study was focused on Ixodes ricinus and Dermacentor reticulatus that infest humans, companion animals and other domestic and wild animals, and transmit disease-causing pathogens. Tick larvae and adult salivary glands were selected for analysis to target tick organs and developmental stages playing a key role during tick life cycle and pathogen infection and transmission. Two I. ricinus (heme lipoprotein and uncharacterized secreted protein) and five D. reticulatus (glypican-like protein, secreted protein involved in homophilic cell adhesion, sulfate/anion exchanger, signal peptidase complex subunit 3, and uncharacterized secreted protein) proteins were identified as the most effective protective antigens based on the criteria of vaccine E > 80%. The putative function of selected protective antigens, which are involved in different biological processes, resulted in vaccines affecting multiple tick developmental stages. These results suggested that the combination of some of these antigens might be considered to increase vaccine efficacy through antigen synergy for the control of tick infestations and potentially affecting pathogen infection and transmission. These antigens were proposed for commercial vaccine development for the control of tick infestations in companion animals, and potentially in other hosts for these tick species.This research was financially supported by Beaphar B.V. (Raalte, Netherlands). MC was funded by the Consejo Superior de Investigaciones Científicas (CSIC), Spain. MV was funded by the Universidad de Castilla–La Mancha (UCLM), Spain.Peer reviewedFrontiers MediaConsejo Superior de Investigaciones Científicas (España)Universidad de Castilla La ManchaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/215380reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3389/fphys.2019.00977Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2153802026-05-22T06:33:51Z
dc.title.none.fl_str_mv A vaccinomics approach for the identification of tick protective antigens for the control of Ixodes ricinus and Dermacentor reticulatus infestations in companion animals
title A vaccinomics approach for the identification of tick protective antigens for the control of Ixodes ricinus and Dermacentor reticulatus infestations in companion animals
spellingShingle A vaccinomics approach for the identification of tick protective antigens for the control of Ixodes ricinus and Dermacentor reticulatus infestations in companion animals
Contreras, Marinela
title_short A vaccinomics approach for the identification of tick protective antigens for the control of Ixodes ricinus and Dermacentor reticulatus infestations in companion animals
title_full A vaccinomics approach for the identification of tick protective antigens for the control of Ixodes ricinus and Dermacentor reticulatus infestations in companion animals
title_fullStr A vaccinomics approach for the identification of tick protective antigens for the control of Ixodes ricinus and Dermacentor reticulatus infestations in companion animals
title_full_unstemmed A vaccinomics approach for the identification of tick protective antigens for the control of Ixodes ricinus and Dermacentor reticulatus infestations in companion animals
title_sort A vaccinomics approach for the identification of tick protective antigens for the control of Ixodes ricinus and Dermacentor reticulatus infestations in companion animals
dc.creator.none.fl_str_mv Contreras, Marinela
Villar, Margarita
Fuente, José de la
author Contreras, Marinela
author_facet Contreras, Marinela
Villar, Margarita
Fuente, José de la
author_role author
author2 Villar, Margarita
Fuente, José de la
author2_role author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas (España)
Universidad de Castilla La Mancha
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description Ticks and tick-borne pathogens affect health and welfare of companion animals worldwide, and some human tick-borne diseases are associated with exposure to domestic animals. Vaccines are the most environmentally friendly alternative to acaracides for the control of tick infestations, and to reduce the risk for tick-borne diseases affecting human and animal health. However, vaccines have not been developed or successfully implemented for most vector-borne diseases. The main limitation for the development of effective vaccines is the identification of protective antigens. To address this limitation, in this study we used an experimental approach combining vaccinomics based on transcriptomics and proteomics data with vaccination trials for the identification of tick protective antigens. The study was focused on Ixodes ricinus and Dermacentor reticulatus that infest humans, companion animals and other domestic and wild animals, and transmit disease-causing pathogens. Tick larvae and adult salivary glands were selected for analysis to target tick organs and developmental stages playing a key role during tick life cycle and pathogen infection and transmission. Two I. ricinus (heme lipoprotein and uncharacterized secreted protein) and five D. reticulatus (glypican-like protein, secreted protein involved in homophilic cell adhesion, sulfate/anion exchanger, signal peptidase complex subunit 3, and uncharacterized secreted protein) proteins were identified as the most effective protective antigens based on the criteria of vaccine E > 80%. The putative function of selected protective antigens, which are involved in different biological processes, resulted in vaccines affecting multiple tick developmental stages. These results suggested that the combination of some of these antigens might be considered to increase vaccine efficacy through antigen synergy for the control of tick infestations and potentially affecting pathogen infection and transmission. These antigens were proposed for commercial vaccine development for the control of tick infestations in companion animals, and potentially in other hosts for these tick species.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/215380
url http://hdl.handle.net/10261/215380
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3389/fphys.2019.00977
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
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