Bluephage, a method for efficient detection of somatic coliphages in one hundred milliliter water samples.

Emerging water quality guidelines and regulations require the absence of somatic coliphages in 100 mL of water, yet the efficiency of standardized methods to test this volume of sample is questionable. A recently described procedure, Bluephage, using a modified E. coli host strain, overcomes some of...

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Detalhes bibliográficos
Autores: Méndez Viera, Javier, Toribio-Avedillo, Daniel, Mangas Casas, Raquel, Martínez González, Judit
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Recursos:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/187403
Acesso em linha:https://hdl.handle.net/2445/187403
Access Level:acceso abierto
Palavra-chave:Qualitat de l'aigua
Contaminació de l'aigua
Virus
Microbiologia aquàtica
Anàlisi de l'aigua
Water quality
Water pollution
Viruses
Water microbiology
Water analysis
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spelling Bluephage, a method for efficient detection of somatic coliphages in one hundred milliliter water samples.Méndez Viera, JavierToribio-Avedillo, DanielMangas Casas, RaquelMartínez González, JuditQualitat de l'aiguaContaminació de l'aiguaVirusMicrobiologia aquàticaAnàlisi de l'aiguaWater qualityWater pollutionVirusesWater microbiologyWater analysisEmerging water quality guidelines and regulations require the absence of somatic coliphages in 100 mL of water, yet the efficiency of standardized methods to test this volume of sample is questionable. A recently described procedure, Bluephage, using a modified E. coli host strain, overcomes some of the methodological limitations of standardized methods. In a maximum of 6.5 hours (2.5 hours for pre-growing the host strain and 4 hours for the presence/absence test), Bluephage allows the direct detection of one plaque-forming unit (PFU) in a 100 mL water sample. The test shows high levels of specificity for somatic coliphages and comparable accuracy with standardized methods.Nature Publishing Group2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/187403Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1038/s41598-020-60071-wScientific Reports, 2020, vol. 10, num. 2020, p. 2977https://doi.org/10.1038/s41598-020-60071-wcc-by (c) Méndez Viera, Javier et al., 2020https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1874032026-05-27T06:46:51Z
dc.title.none.fl_str_mv Bluephage, a method for efficient detection of somatic coliphages in one hundred milliliter water samples.
title Bluephage, a method for efficient detection of somatic coliphages in one hundred milliliter water samples.
spellingShingle Bluephage, a method for efficient detection of somatic coliphages in one hundred milliliter water samples.
Méndez Viera, Javier
Qualitat de l'aigua
Contaminació de l'aigua
Virus
Microbiologia aquàtica
Anàlisi de l'aigua
Water quality
Water pollution
Viruses
Water microbiology
Water analysis
title_short Bluephage, a method for efficient detection of somatic coliphages in one hundred milliliter water samples.
title_full Bluephage, a method for efficient detection of somatic coliphages in one hundred milliliter water samples.
title_fullStr Bluephage, a method for efficient detection of somatic coliphages in one hundred milliliter water samples.
title_full_unstemmed Bluephage, a method for efficient detection of somatic coliphages in one hundred milliliter water samples.
title_sort Bluephage, a method for efficient detection of somatic coliphages in one hundred milliliter water samples.
dc.creator.none.fl_str_mv Méndez Viera, Javier
Toribio-Avedillo, Daniel
Mangas Casas, Raquel
Martínez González, Judit
author Méndez Viera, Javier
author_facet Méndez Viera, Javier
Toribio-Avedillo, Daniel
Mangas Casas, Raquel
Martínez González, Judit
author_role author
author2 Toribio-Avedillo, Daniel
Mangas Casas, Raquel
Martínez González, Judit
author2_role author
author
author
dc.subject.none.fl_str_mv Qualitat de l'aigua
Contaminació de l'aigua
Virus
Microbiologia aquàtica
Anàlisi de l'aigua
Water quality
Water pollution
Viruses
Water microbiology
Water analysis
topic Qualitat de l'aigua
Contaminació de l'aigua
Virus
Microbiologia aquàtica
Anàlisi de l'aigua
Water quality
Water pollution
Viruses
Water microbiology
Water analysis
description Emerging water quality guidelines and regulations require the absence of somatic coliphages in 100 mL of water, yet the efficiency of standardized methods to test this volume of sample is questionable. A recently described procedure, Bluephage, using a modified E. coli host strain, overcomes some of the methodological limitations of standardized methods. In a maximum of 6.5 hours (2.5 hours for pre-growing the host strain and 4 hours for the presence/absence test), Bluephage allows the direct detection of one plaque-forming unit (PFU) in a 100 mL water sample. The test shows high levels of specificity for somatic coliphages and comparable accuracy with standardized methods.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/187403
url https://hdl.handle.net/2445/187403
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1038/s41598-020-60071-w
Scientific Reports, 2020, vol. 10, num. 2020, p. 2977
https://doi.org/10.1038/s41598-020-60071-w
dc.rights.none.fl_str_mv cc-by (c) Méndez Viera, Javier et al., 2020
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Méndez Viera, Javier et al., 2020
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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