Inter-hairpin linker sequences determine the structure of the ββ-solenoid fold: a "bottom-up" study of pneumococcal LytA choline-binding module

14 p.-9 fig.-4 tab.

Detalles Bibliográficos
Autores: Maestro, Beatriz, Zamora-Carreras, H., Jiménez, M. Angeles, Sanz, Jesús M.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/250186
Acceso en línea:http://hdl.handle.net/10261/250186
Access Level:acceso abierto
Palabra clave:Choline-binding proteins
NMR
Repeat proteins
Streptococcus pneumoniae
Tryptophan peptides
β-Hairpin
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spelling Inter-hairpin linker sequences determine the structure of the ββ-solenoid fold: a "bottom-up" study of pneumococcal LytA choline-binding moduleMaestro, BeatrizZamora-Carreras, H.Jiménez, M. AngelesSanz, Jesús M.Choline-binding proteinsNMRRepeat proteinsStreptococcus pneumoniaeTryptophan peptidesβ-Hairpin14 p.-9 fig.-4 tab.The ββ-solenoid structures are part of many proteins involved in the recognition of bacterial cell wall. They are elongated polypeptides consisting of repeated β-hairpins connected by linker sequences and disposed around a superhelical axis stabilised by short-range interactions. Among the most studied ββ-solenoids are those belonging to the family of choline-binding modules (CBMs) from the respiratory pathogen Streptococcus pneumoniae (pneumococcus) and its bacteriophages, and their properties have been employed to develop several biotechnological and biomedical tools. We have carried out a theoretical, spectroscopic and thermodynamic study of the ββ-solenoid structure of the CBM from the pneumococcal LytA autolysin using peptides of increasing length containing 1–3 repeats of this structure. Our results show that hints of native-like tertiary structure are only observed with a minimum of three β-hairpins, corresponding to one turn of the solenoid superhelix, and identify the linker sequences between hairpins as the major directors of the solenoid folding. This study paves the way for the rational structural engineering of ββ-solenoids aimed to find novel applications.Funding sources: grants BIO2016-79323-R, CTQ2017-84371P and PID2019-105126RB-I00 (Agencia Estatal de Investigacion AEI/FEDER-EU-10.13039/ 501100011033-, and Ministerio de Ciencia e Innovación, Spain) and Intramural incorporation project #201820I132 (CSIC, Spain).Peer reviewedElsevierAgencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)Consejo Superior de Investigaciones Científicas (España)Maestro, Beatriz [0000-0001-5317-650X]Zamora-Carreras, H. [0000-0002-9141-2750]Jiménez, M. Angeles [0000-0001-6835-5850]Sanz, Jesús M. [0000-0002-4421-9376]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versionPostprintinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/250186reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-79323-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/CTQ2017-84371Pinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105126RB-I00https://doi.org/10.1016/j.ijbiomac.2021.08.223Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2501862026-05-22T06:33:51Z
dc.title.none.fl_str_mv Inter-hairpin linker sequences determine the structure of the ββ-solenoid fold: a "bottom-up" study of pneumococcal LytA choline-binding module
title Inter-hairpin linker sequences determine the structure of the ββ-solenoid fold: a "bottom-up" study of pneumococcal LytA choline-binding module
spellingShingle Inter-hairpin linker sequences determine the structure of the ββ-solenoid fold: a "bottom-up" study of pneumococcal LytA choline-binding module
Maestro, Beatriz
Choline-binding proteins
NMR
Repeat proteins
Streptococcus pneumoniae
Tryptophan peptides
β-Hairpin
title_short Inter-hairpin linker sequences determine the structure of the ββ-solenoid fold: a "bottom-up" study of pneumococcal LytA choline-binding module
title_full Inter-hairpin linker sequences determine the structure of the ββ-solenoid fold: a "bottom-up" study of pneumococcal LytA choline-binding module
title_fullStr Inter-hairpin linker sequences determine the structure of the ββ-solenoid fold: a "bottom-up" study of pneumococcal LytA choline-binding module
title_full_unstemmed Inter-hairpin linker sequences determine the structure of the ββ-solenoid fold: a "bottom-up" study of pneumococcal LytA choline-binding module
title_sort Inter-hairpin linker sequences determine the structure of the ββ-solenoid fold: a "bottom-up" study of pneumococcal LytA choline-binding module
dc.creator.none.fl_str_mv Maestro, Beatriz
Zamora-Carreras, H.
Jiménez, M. Angeles
Sanz, Jesús M.
author Maestro, Beatriz
author_facet Maestro, Beatriz
Zamora-Carreras, H.
Jiménez, M. Angeles
Sanz, Jesús M.
author_role author
author2 Zamora-Carreras, H.
Jiménez, M. Angeles
Sanz, Jesús M.
author2_role author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
Consejo Superior de Investigaciones Científicas (España)
Maestro, Beatriz [0000-0001-5317-650X]
Zamora-Carreras, H. [0000-0002-9141-2750]
Jiménez, M. Angeles [0000-0001-6835-5850]
Sanz, Jesús M. [0000-0002-4421-9376]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Choline-binding proteins
NMR
Repeat proteins
Streptococcus pneumoniae
Tryptophan peptides
β-Hairpin
topic Choline-binding proteins
NMR
Repeat proteins
Streptococcus pneumoniae
Tryptophan peptides
β-Hairpin
description 14 p.-9 fig.-4 tab.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
Postprint
info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/acceptedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/250186
url http://hdl.handle.net/10261/250186
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-79323-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/CTQ2017-84371P
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105126RB-I00
https://doi.org/10.1016/j.ijbiomac.2021.08.223
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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