Interplay between podoplanin, CD44s and CD44v in squamous carcinoma cells

Podoplanin and CD44 are transmembrane glycoproteins involved in inflammation and cancer. In this paper, we report that podoplanin is coordinately expressed with the CD44 standard (CD44s) and variant (CD44v) isoforms in vivo-in hyperplastic skin after a pro-inflammatory stimulus with 12-O-tetradecano...

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Detalles Bibliográficos
Autores: Montero-Montero, Lucía, Renart, Jaime, Ramírez, Andrés, Ramos, Carmen, Shamhood, Mariam, Jarcovsky, Rocío, Quintanilla, Miguel, Martín-Villar, Ester
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/695741
Acceso en línea:http://hdl.handle.net/10486/695741
https://dx.doi.org/10.3390/cells9102200
Access Level:acceso abierto
Palabra clave:CD44s
CD44v
podoplanin
protein–protein interaction
squamous carcinoma cells
Biología y Biomedicina / Biología
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spelling Interplay between podoplanin, CD44s and CD44v in squamous carcinoma cellsMontero-Montero, LucíaRenart, JaimeRamírez, AndrésRamos, CarmenShamhood, MariamJarcovsky, RocíoQuintanilla, MiguelMartín-Villar, EsterCD44sCD44vpodoplaninprotein–protein interactionsquamous carcinoma cellsBiología y Biomedicina / BiologíaPodoplanin and CD44 are transmembrane glycoproteins involved in inflammation and cancer. In this paper, we report that podoplanin is coordinately expressed with the CD44 standard (CD44s) and variant (CD44v) isoforms in vivo-in hyperplastic skin after a pro-inflammatory stimulus with 12-O-tetradecanoylphorbol-13-acetate (TPA)-and in vitro-in cell lines representative of different stages of mouse-skin chemical carcinogenesis, as well as in human squamous carcinoma cell (SCC) lines. Moreover, we identify CD44v10 in the mouse-skin carcinogenesis model as the only CD44 variant isoform expressed in highly aggressive spindle carcinoma cell lines together with CD44s and podoplanin. We also characterized CD44v3-10, CD44v6-10 and CD44v8-10 as the major variant isoforms co-expressed with CD44s and podoplanin in human SCC cell lines. Immunofluorescence confocal microscopy experiments show that these CD44v isoforms colocalize with podoplanin at plasma membrane protrusions and cell-cell contacts of SCC cells, as previously reported for CD44s. Furthermore, CD44v isoforms colocalize with podoplanin in chemically induced mouse-skin SCCs in vivo. Co-immunoprecipitation experiments indicate that podoplanin physically binds to CD44v3-10, CD44v6-10 and CD44v8-10 isoforms, as well as to CD44s. Podoplanin-CD44 interaction is mediated by the transmembrane and cytosolic regions and is negatively modulated by glycosylation of the extracellular domain. These results point to a functional interplay of podoplanin with both CD44v and CD44s isoforms in SCCs and give insight into the regulation of the podoplanin-CD44 association.This research was funded by a grant from Ministerio de Ciencia, Innovación y Universidades of Spain (SAF2017-84183-R) and from Fundación Universidad Francisco de Vitoria (UFV2020-28)MDPI, Basel, Switzerland20202020-09-29research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/695741https://dx.doi.org/10.3390/cells9102200reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/6957412026-06-23T12:46:27Z
dc.title.none.fl_str_mv Interplay between podoplanin, CD44s and CD44v in squamous carcinoma cells
title Interplay between podoplanin, CD44s and CD44v in squamous carcinoma cells
spellingShingle Interplay between podoplanin, CD44s and CD44v in squamous carcinoma cells
Montero-Montero, Lucía
CD44s
CD44v
podoplanin
protein–protein interaction
squamous carcinoma cells
Biología y Biomedicina / Biología
title_short Interplay between podoplanin, CD44s and CD44v in squamous carcinoma cells
title_full Interplay between podoplanin, CD44s and CD44v in squamous carcinoma cells
title_fullStr Interplay between podoplanin, CD44s and CD44v in squamous carcinoma cells
title_full_unstemmed Interplay between podoplanin, CD44s and CD44v in squamous carcinoma cells
title_sort Interplay between podoplanin, CD44s and CD44v in squamous carcinoma cells
dc.creator.none.fl_str_mv Montero-Montero, Lucía
Renart, Jaime
Ramírez, Andrés
Ramos, Carmen
Shamhood, Mariam
Jarcovsky, Rocío
Quintanilla, Miguel
Martín-Villar, Ester
author Montero-Montero, Lucía
author_facet Montero-Montero, Lucía
Renart, Jaime
Ramírez, Andrés
Ramos, Carmen
Shamhood, Mariam
Jarcovsky, Rocío
Quintanilla, Miguel
Martín-Villar, Ester
author_role author
author2 Renart, Jaime
Ramírez, Andrés
Ramos, Carmen
Shamhood, Mariam
Jarcovsky, Rocío
Quintanilla, Miguel
Martín-Villar, Ester
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CD44s
CD44v
podoplanin
protein–protein interaction
squamous carcinoma cells
Biología y Biomedicina / Biología
topic CD44s
CD44v
podoplanin
protein–protein interaction
squamous carcinoma cells
Biología y Biomedicina / Biología
description Podoplanin and CD44 are transmembrane glycoproteins involved in inflammation and cancer. In this paper, we report that podoplanin is coordinately expressed with the CD44 standard (CD44s) and variant (CD44v) isoforms in vivo-in hyperplastic skin after a pro-inflammatory stimulus with 12-O-tetradecanoylphorbol-13-acetate (TPA)-and in vitro-in cell lines representative of different stages of mouse-skin chemical carcinogenesis, as well as in human squamous carcinoma cell (SCC) lines. Moreover, we identify CD44v10 in the mouse-skin carcinogenesis model as the only CD44 variant isoform expressed in highly aggressive spindle carcinoma cell lines together with CD44s and podoplanin. We also characterized CD44v3-10, CD44v6-10 and CD44v8-10 as the major variant isoforms co-expressed with CD44s and podoplanin in human SCC cell lines. Immunofluorescence confocal microscopy experiments show that these CD44v isoforms colocalize with podoplanin at plasma membrane protrusions and cell-cell contacts of SCC cells, as previously reported for CD44s. Furthermore, CD44v isoforms colocalize with podoplanin in chemically induced mouse-skin SCCs in vivo. Co-immunoprecipitation experiments indicate that podoplanin physically binds to CD44v3-10, CD44v6-10 and CD44v8-10 isoforms, as well as to CD44s. Podoplanin-CD44 interaction is mediated by the transmembrane and cytosolic regions and is negatively modulated by glycosylation of the extracellular domain. These results point to a functional interplay of podoplanin with both CD44v and CD44s isoforms in SCCs and give insight into the regulation of the podoplanin-CD44 association.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-09-29
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/695741
https://dx.doi.org/10.3390/cells9102200
url http://hdl.handle.net/10486/695741
https://dx.doi.org/10.3390/cells9102200
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI, Basel, Switzerland
publisher.none.fl_str_mv MDPI, Basel, Switzerland
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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