HetR-dependent and -independent expression of heterocyst-related genes in an Anabaena strain overproducing the NtcA transcription factor

Heterocyst development in the cyanobacterium Anabaena sp. strain PCC 7120 depends on both the global nitrogen control transcription factor NtcA and the cell differentiation regulatory protein HetR, with expression of ntcA and hetR being dependent on each other. In this study we constructed strains t...

Descripción completa

Detalles Bibliográficos
Autores: Olmedo Verd, Elvira de, Flores García, Enrique, Herrero Moreno, Antonia, Muro Pastor, Alicia María
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2005
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/62939
Acceso en línea:http://hdl.handle.net/11441/62939
https://doi.org/10.1128/JB.187.6.1985–1991.2005
Access Level:acceso abierto
id ES_ec64ec9dd3fce43ab584ed8a4479db05
oai_identifier_str oai:idus.us.es:11441/62939
network_acronym_str ES
network_name_str España
repository_id_str
spelling HetR-dependent and -independent expression of heterocyst-related genes in an Anabaena strain overproducing the NtcA transcription factorOlmedo Verd, Elvira deFlores García, EnriqueHerrero Moreno, AntoniaMuro Pastor, Alicia MaríaHeterocyst development in the cyanobacterium Anabaena sp. strain PCC 7120 depends on both the global nitrogen control transcription factor NtcA and the cell differentiation regulatory protein HetR, with expression of ntcA and hetR being dependent on each other. In this study we constructed strains that constitutively express the ntcA gene leading to high levels of NtcA protein irrespective of the nitrogen source, and we analyzed the elects of such NtcA levels on heterocyst diferentiation. In the NtcA-overproducing strain, heterocyst differentiation, induction of NtcA-dependent heterocyst development genes or operons such as devBCA or the cox2 operon, and NtcA-dependent excision of the 11-kb nifD-intervening element only took place under nitrogen deficiency. Although functional heterocysts were produced in response to nitrogen step-down, the NtcA over-producing strain could not grow diazotrophically. Overexpression of ntcA in a hetR background promoted expression of devBCA in response to ammonium withdrawal and excision of the 11-kb element even in the presence of combined nitrogen. Our results show that some NtcA-dependent heterocyst-related genes can be expressed independently of HetR.Ministerio de Ciencia y Tecnología BMC2001-0509American Society for MicrobiologyBioquímica Vegetal y Biología MolecularMinisterio de Ciencia y Tecnología (MCYT). España2005info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/11441/62939https://doi.org/10.1128/JB.187.6.1985–1991.2005reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésJournal of Bacteriology, 187 (6), 1985-1991.BMC2001-0509http://dx.doi.org/10.1128/JB.187.6.1985–1991.2005info:eu-repo/semantics/openAccessoai:idus.us.es:11441/629392026-06-17T12:51:07Z
dc.title.none.fl_str_mv HetR-dependent and -independent expression of heterocyst-related genes in an Anabaena strain overproducing the NtcA transcription factor
title HetR-dependent and -independent expression of heterocyst-related genes in an Anabaena strain overproducing the NtcA transcription factor
spellingShingle HetR-dependent and -independent expression of heterocyst-related genes in an Anabaena strain overproducing the NtcA transcription factor
Olmedo Verd, Elvira de
title_short HetR-dependent and -independent expression of heterocyst-related genes in an Anabaena strain overproducing the NtcA transcription factor
title_full HetR-dependent and -independent expression of heterocyst-related genes in an Anabaena strain overproducing the NtcA transcription factor
title_fullStr HetR-dependent and -independent expression of heterocyst-related genes in an Anabaena strain overproducing the NtcA transcription factor
title_full_unstemmed HetR-dependent and -independent expression of heterocyst-related genes in an Anabaena strain overproducing the NtcA transcription factor
title_sort HetR-dependent and -independent expression of heterocyst-related genes in an Anabaena strain overproducing the NtcA transcription factor
dc.creator.none.fl_str_mv Olmedo Verd, Elvira de
Flores García, Enrique
Herrero Moreno, Antonia
Muro Pastor, Alicia María
author Olmedo Verd, Elvira de
author_facet Olmedo Verd, Elvira de
Flores García, Enrique
Herrero Moreno, Antonia
Muro Pastor, Alicia María
author_role author
author2 Flores García, Enrique
Herrero Moreno, Antonia
Muro Pastor, Alicia María
author2_role author
author
author
dc.contributor.none.fl_str_mv Bioquímica Vegetal y Biología Molecular
Ministerio de Ciencia y Tecnología (MCYT). España
description Heterocyst development in the cyanobacterium Anabaena sp. strain PCC 7120 depends on both the global nitrogen control transcription factor NtcA and the cell differentiation regulatory protein HetR, with expression of ntcA and hetR being dependent on each other. In this study we constructed strains that constitutively express the ntcA gene leading to high levels of NtcA protein irrespective of the nitrogen source, and we analyzed the elects of such NtcA levels on heterocyst diferentiation. In the NtcA-overproducing strain, heterocyst differentiation, induction of NtcA-dependent heterocyst development genes or operons such as devBCA or the cox2 operon, and NtcA-dependent excision of the 11-kb nifD-intervening element only took place under nitrogen deficiency. Although functional heterocysts were produced in response to nitrogen step-down, the NtcA over-producing strain could not grow diazotrophically. Overexpression of ntcA in a hetR background promoted expression of devBCA in response to ammonium withdrawal and excision of the 11-kb element even in the presence of combined nitrogen. Our results show that some NtcA-dependent heterocyst-related genes can be expressed independently of HetR.
publishDate 2005
dc.date.none.fl_str_mv 2005
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11441/62939
https://doi.org/10.1128/JB.187.6.1985–1991.2005
url http://hdl.handle.net/11441/62939
https://doi.org/10.1128/JB.187.6.1985–1991.2005
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Journal of Bacteriology, 187 (6), 1985-1991.
BMC2001-0509
http://dx.doi.org/10.1128/JB.187.6.1985–1991.2005
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869423310979203072
score 15,301603

Similares en LA Referencia