Digestion of organosulfur compounds of two radish microgreen cultivars grown under different light treatments
Radishes are rich in health-promoting organosulfur compounds. This study investigated the effects of gastrointestinal digestion (GID) on glucosinolates (GSLs) and isothiocyanates (ITCs) of two radish microgreens cultivars (Raphanus sativus; cv. Daikon, and cv. Red Rambo) grown in a closed vertical s...
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/397177 |
| Acceso en línea: | http://hdl.handle.net/10261/397177 https://api.elsevier.com/content/abstract/scopus_id/105008503373 |
| Access Level: | acceso abierto |
| Palabra clave: | Glucosinolates Isothiocyanates INFOGEST Growing lights Structural equation modelling |
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Digestion of organosulfur compounds of two radish microgreen cultivars grown under different light treatmentsSilva, MartaDomínguez-Perles, RaúlMoreno, Diego A.Viegas, OlgaFaria, Miguel A.Carvalho, Susana M. P.Ferreira, Isabel M. P. L. V. O.GlucosinolatesIsothiocyanatesINFOGESTGrowing lightsStructural equation modellingRadishes are rich in health-promoting organosulfur compounds. This study investigated the effects of gastrointestinal digestion (GID) on glucosinolates (GSLs) and isothiocyanates (ITCs) of two radish microgreens cultivars (Raphanus sativus; cv. Daikon, and cv. Red Rambo) grown in a closed vertical system under either White (W) or Red+Blue (R + B) LEDs, combined with varying applications of UV-C radiation (0, 1, or 3 pulses). The individual and combined effects of LEDs and UV-C on GSLs and ITCs were evaluated before and after GID (INFOGEST static in vitro GID model). Relationships between compounds before/after digestion were explored through Structural Equation Modelling (SEM). Both cultivars are good sources of aliphatic GSLs (Daikon: 513.4 ± 23.1-681.5 ± 31.9, Red Rambo: 447.5 ± 38.5-532.0 ± 20.2 mg 100 g-1) and ITCs (Daikon: 0.9 ± 0.1-1.8 ± 0.3, Red Rambo: 1.3 ± 0.2-5.2 ± 1.0 mg 100 g-1). LED type was more impactful than UV-C radiation. Generally, R + B LEDs enhanced GSLs due to increasing dehydroerucin in Daikon, while improving ITCs in Red Rambo. Post-digestion, Red Rambo showed markedly higher sulforaphene levels compared to Daikon. Red Rambo's SEM significantly confirmed the conversion of glucoraphenin into sulforaphene and sulforaphane during GID. In Daikon, no significant relationships between compounds before/after GID were found. These findings suggest cultivar-specific metabolic pathways and responses to light, which can be optimized to enhance the accumulation of health-related compounds. The innovative use of SEM provided deeper insights into the metabolic conversions occurring during GID. The abundant sulforaphene levels from Red Rambo highlight this cultivar as an excellent source of this metabolite and its potential health benefitsThis work was supported through the strategic projects (UIDB/50006/2020, UIDP/50006/2020, UIDB/05748/2020, UIDP/05748/2020) funded by FCT/MCTES through national funds, and the R&D projects SALIVA+ (2022.08978.PTDC) and SNAP I&D (NORTE-01-0145-FEDER-000085). Marta Silva wishes to thank FCT for the Ph.D. Grant (10.54499/2020.05266.BD). M. A. Faria thanks FCT the researcher contract CEECINSTLA/00029/2022. D.A.M. would also like to express his gratitude to HORTNEX project that partially funded this research, under the AGROALNEXT (Agroalnext_2022_027) programme of MCIN and funded by the European Union “NextGenerationEU” (PRTR-C17·I1) and by the Generalitat ValencianaPeer reviewedElsevierFundação para a Ciência e a Tecnologia (Portugal)European CommissionGeneralitat ValencianaMinisterio de Ciencia e Innovación (España)Domínguez-Perles, Raúl [0000-0001-6232-712X]Moreno, Diego A. [0000-0002-6547-8764]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/397177https://api.elsevier.com/content/abstract/scopus_id/105008503373reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésFood research international (Ottawa, Ont.)The underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.1016/j.foodres.2025.116831https://doi.org/10.1016/j.foodres.2025.116831Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3971772026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
Digestion of organosulfur compounds of two radish microgreen cultivars grown under different light treatments |
| title |
Digestion of organosulfur compounds of two radish microgreen cultivars grown under different light treatments |
| spellingShingle |
Digestion of organosulfur compounds of two radish microgreen cultivars grown under different light treatments Silva, Marta Glucosinolates Isothiocyanates INFOGEST Growing lights Structural equation modelling |
| title_short |
Digestion of organosulfur compounds of two radish microgreen cultivars grown under different light treatments |
| title_full |
Digestion of organosulfur compounds of two radish microgreen cultivars grown under different light treatments |
| title_fullStr |
Digestion of organosulfur compounds of two radish microgreen cultivars grown under different light treatments |
| title_full_unstemmed |
Digestion of organosulfur compounds of two radish microgreen cultivars grown under different light treatments |
| title_sort |
Digestion of organosulfur compounds of two radish microgreen cultivars grown under different light treatments |
| dc.creator.none.fl_str_mv |
Silva, Marta Domínguez-Perles, Raúl Moreno, Diego A. Viegas, Olga Faria, Miguel A. Carvalho, Susana M. P. Ferreira, Isabel M. P. L. V. O. |
| author |
Silva, Marta |
| author_facet |
Silva, Marta Domínguez-Perles, Raúl Moreno, Diego A. Viegas, Olga Faria, Miguel A. Carvalho, Susana M. P. Ferreira, Isabel M. P. L. V. O. |
| author_role |
author |
| author2 |
Domínguez-Perles, Raúl Moreno, Diego A. Viegas, Olga Faria, Miguel A. Carvalho, Susana M. P. Ferreira, Isabel M. P. L. V. O. |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Fundação para a Ciência e a Tecnologia (Portugal) European Commission Generalitat Valenciana Ministerio de Ciencia e Innovación (España) Domínguez-Perles, Raúl [0000-0001-6232-712X] Moreno, Diego A. [0000-0002-6547-8764] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Glucosinolates Isothiocyanates INFOGEST Growing lights Structural equation modelling |
| topic |
Glucosinolates Isothiocyanates INFOGEST Growing lights Structural equation modelling |
| description |
Radishes are rich in health-promoting organosulfur compounds. This study investigated the effects of gastrointestinal digestion (GID) on glucosinolates (GSLs) and isothiocyanates (ITCs) of two radish microgreens cultivars (Raphanus sativus; cv. Daikon, and cv. Red Rambo) grown in a closed vertical system under either White (W) or Red+Blue (R + B) LEDs, combined with varying applications of UV-C radiation (0, 1, or 3 pulses). The individual and combined effects of LEDs and UV-C on GSLs and ITCs were evaluated before and after GID (INFOGEST static in vitro GID model). Relationships between compounds before/after digestion were explored through Structural Equation Modelling (SEM). Both cultivars are good sources of aliphatic GSLs (Daikon: 513.4 ± 23.1-681.5 ± 31.9, Red Rambo: 447.5 ± 38.5-532.0 ± 20.2 mg 100 g-1) and ITCs (Daikon: 0.9 ± 0.1-1.8 ± 0.3, Red Rambo: 1.3 ± 0.2-5.2 ± 1.0 mg 100 g-1). LED type was more impactful than UV-C radiation. Generally, R + B LEDs enhanced GSLs due to increasing dehydroerucin in Daikon, while improving ITCs in Red Rambo. Post-digestion, Red Rambo showed markedly higher sulforaphene levels compared to Daikon. Red Rambo's SEM significantly confirmed the conversion of glucoraphenin into sulforaphene and sulforaphane during GID. In Daikon, no significant relationships between compounds before/after GID were found. These findings suggest cultivar-specific metabolic pathways and responses to light, which can be optimized to enhance the accumulation of health-related compounds. The innovative use of SEM provided deeper insights into the metabolic conversions occurring during GID. The abundant sulforaphene levels from Red Rambo highlight this cultivar as an excellent source of this metabolite and its potential health benefits |
| publishDate |
2025 |
| dc.date.none.fl_str_mv |
2025 2025 2025 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/397177 https://api.elsevier.com/content/abstract/scopus_id/105008503373 |
| url |
http://hdl.handle.net/10261/397177 https://api.elsevier.com/content/abstract/scopus_id/105008503373 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Food research international (Ottawa, Ont.) The underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.1016/j.foodres.2025.116831 https://doi.org/10.1016/j.foodres.2025.116831 Sí |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Elsevier |
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Elsevier |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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