CD69 does not affect the extent of T cell priming

CD69 is rapidly upregulated on T cells upon activation. In this work we show that this is also the case for CD69 expression on dendritic cells (DC). Thus, the expression kinetics of CD69 on both cell types is reminiscent of the one of costimulatory molecules. Using mouse models of transgenic T cells...

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Detalles Bibliográficos
Autores: Alari-Pahissa, Elisenda, Notario, Laura, Lorente, Elena, Vega-Ramos, Javier, Justel, Ana, Lopez, Daniel, Villadangos, José A, Lauzurica, Pilar
Tipo de recurso: artículo
Fecha de publicación:2012
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/6703
Acceso en línea:http://hdl.handle.net/20.500.12105/6703
Access Level:acceso abierto
Palabra clave:Adoptive Transfer
Animals
Antigen Presentation
Antigens
Antigens, CD
Antigens, Differentiation, T-Lymphocyte
CD8-Positive T-Lymphocytes
Cell Proliferation
Cells, Cultured
Dendritic Cells
Female
Flow Cytometry
Lectins, C-Type
Lipopolysaccharides
Lymph Nodes
Lymphocyte Activation
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Oligodeoxyribonucleotides
T-Lymphocytes
Up-Regulation
Vaccinia
Vaccinia virus
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oai_identifier_str oai:repisalud.isciii.es:20.500.12105/6703
network_acronym_str ES
network_name_str España
repository_id_str
spelling CD69 does not affect the extent of T cell primingAlari-Pahissa, ElisendaNotario, LauraLorente, ElenaVega-Ramos, JavierJustel, AnaLopez, DanielVilladangos, José ALauzurica, PilarAdoptive TransferAnimalsAntigen PresentationAntigensAntigens, CDAntigens, Differentiation, T-LymphocyteCD8-Positive T-LymphocytesCell ProliferationCells, CulturedDendritic CellsFemaleFlow CytometryLectins, C-TypeLipopolysaccharidesLymph NodesLymphocyte ActivationMaleMiceMice, Inbred BALB CMice, Inbred C57BLMice, KnockoutMice, TransgenicOligodeoxyribonucleotidesT-LymphocytesUp-RegulationVacciniaVaccinia virusCD69 is rapidly upregulated on T cells upon activation. In this work we show that this is also the case for CD69 expression on dendritic cells (DC). Thus, the expression kinetics of CD69 on both cell types is reminiscent of the one of costimulatory molecules. Using mouse models of transgenic T cells, we aimed at evaluating the effect of monoclonal antibody (MAb)-based targeting and gene deficiency of CD69 expressed by either DC or T cells on the extent of antigen (Ag)-specific T cell priming, which could be the result of a putative role in costimulation as well as on DC maturation and Ag-processing and presentation. CD69 targeting or deficiency of DC did not affect their expression of costimulatory molecules nor their capacity to induce Ag-specific T cell proliferation in in vitro assays. Also, CD69 targeting or deficiency of transgenic T cells did not affect the minimal proliferative dose for different peptide agonists in vitro. In in vivo models of transgenic T cell transfer and local Ag injection, CD69 deficiency of transferred T cells did not affect the extent of the proliferative response in Ag-draining lymph nodes (LN). In agreement with these results, CD69 MAb targeting or gene deficiency of Vaccinia-virus (VACV) infected mice did not affect the endogenous formation of virus-specific CD8(+) T cell populations at the peak of the primary immune response. Altogether our results argue against a possible role in costimulation or an effect on Ag processing and presentation for CD69.Public Library of Science (PLOS)20182018-11-2220122012-10-3020122012-10-30journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/6703reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)InglésengES SAF2007-64310 Not availableopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/67032026-06-12T12:43:37Z
dc.title.none.fl_str_mv CD69 does not affect the extent of T cell priming
title CD69 does not affect the extent of T cell priming
spellingShingle CD69 does not affect the extent of T cell priming
Alari-Pahissa, Elisenda
Adoptive Transfer
Animals
Antigen Presentation
Antigens
Antigens, CD
Antigens, Differentiation, T-Lymphocyte
CD8-Positive T-Lymphocytes
Cell Proliferation
Cells, Cultured
Dendritic Cells
Female
Flow Cytometry
Lectins, C-Type
Lipopolysaccharides
Lymph Nodes
Lymphocyte Activation
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Oligodeoxyribonucleotides
T-Lymphocytes
Up-Regulation
Vaccinia
Vaccinia virus
title_short CD69 does not affect the extent of T cell priming
title_full CD69 does not affect the extent of T cell priming
title_fullStr CD69 does not affect the extent of T cell priming
title_full_unstemmed CD69 does not affect the extent of T cell priming
title_sort CD69 does not affect the extent of T cell priming
dc.creator.none.fl_str_mv Alari-Pahissa, Elisenda
Notario, Laura
Lorente, Elena
Vega-Ramos, Javier
Justel, Ana
Lopez, Daniel
Villadangos, José A
Lauzurica, Pilar
author Alari-Pahissa, Elisenda
author_facet Alari-Pahissa, Elisenda
Notario, Laura
Lorente, Elena
Vega-Ramos, Javier
Justel, Ana
Lopez, Daniel
Villadangos, José A
Lauzurica, Pilar
author_role author
author2 Notario, Laura
Lorente, Elena
Vega-Ramos, Javier
Justel, Ana
Lopez, Daniel
Villadangos, José A
Lauzurica, Pilar
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Adoptive Transfer
Animals
Antigen Presentation
Antigens
Antigens, CD
Antigens, Differentiation, T-Lymphocyte
CD8-Positive T-Lymphocytes
Cell Proliferation
Cells, Cultured
Dendritic Cells
Female
Flow Cytometry
Lectins, C-Type
Lipopolysaccharides
Lymph Nodes
Lymphocyte Activation
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Oligodeoxyribonucleotides
T-Lymphocytes
Up-Regulation
Vaccinia
Vaccinia virus
topic Adoptive Transfer
Animals
Antigen Presentation
Antigens
Antigens, CD
Antigens, Differentiation, T-Lymphocyte
CD8-Positive T-Lymphocytes
Cell Proliferation
Cells, Cultured
Dendritic Cells
Female
Flow Cytometry
Lectins, C-Type
Lipopolysaccharides
Lymph Nodes
Lymphocyte Activation
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Oligodeoxyribonucleotides
T-Lymphocytes
Up-Regulation
Vaccinia
Vaccinia virus
description CD69 is rapidly upregulated on T cells upon activation. In this work we show that this is also the case for CD69 expression on dendritic cells (DC). Thus, the expression kinetics of CD69 on both cell types is reminiscent of the one of costimulatory molecules. Using mouse models of transgenic T cells, we aimed at evaluating the effect of monoclonal antibody (MAb)-based targeting and gene deficiency of CD69 expressed by either DC or T cells on the extent of antigen (Ag)-specific T cell priming, which could be the result of a putative role in costimulation as well as on DC maturation and Ag-processing and presentation. CD69 targeting or deficiency of DC did not affect their expression of costimulatory molecules nor their capacity to induce Ag-specific T cell proliferation in in vitro assays. Also, CD69 targeting or deficiency of transgenic T cells did not affect the minimal proliferative dose for different peptide agonists in vitro. In in vivo models of transgenic T cell transfer and local Ag injection, CD69 deficiency of transferred T cells did not affect the extent of the proliferative response in Ag-draining lymph nodes (LN). In agreement with these results, CD69 MAb targeting or gene deficiency of Vaccinia-virus (VACV) infected mice did not affect the endogenous formation of virus-specific CD8(+) T cell populations at the peak of the primary immune response. Altogether our results argue against a possible role in costimulation or an effect on Ag processing and presentation for CD69.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-10-30
2012
2012-10-30
2018
2018-11-22
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/6703
url http://hdl.handle.net/20.500.12105/6703
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv ES SAF2007-64310 Not available
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLOS)
publisher.none.fl_str_mv Public Library of Science (PLOS)
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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