Mitochondrial implications in human pregnancies with intrauterine growth restriction and associated cardiac remodelling

Intrauterine growth restriction (IUGR) is an obstetric complication characterised by placental insufficiency and secondary cardiovascular remodelling that can lead to cardiomyopathy in adulthood. Despite its aetiology and potential therapeutics are poorly understood, bioenergetic deficits have been...

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Autores: Guitart Mampel, Mariona, Juárez Flores, Diana Luz, Youssef, Lina, Morén Núñez, Constanza, Garcia Otero, Laura, Roca Agujetas, Vicente, Catalán García, Marc, González Casacuberta, Ingrid, Tobías, Ester, Milisenda, José, Grau Junyent, Josep M. (Josep Maria), Crispi Brillas, Fàtima, Gratacós Solsona, Eduard, Cardellach, Francesc, Garrabou Tornos, Glòria
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/149180
Acceso en línea:https://hdl.handle.net/2445/149180
Access Level:acceso abierto
Palabra clave:Bioenergètica
Mitocondris
Embaràs
Bioenergetics
Mitochondria
Pregnancy
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spelling Mitochondrial implications in human pregnancies with intrauterine growth restriction and associated cardiac remodellingGuitart Mampel, MarionaJuárez Flores, Diana LuzYoussef, LinaMorén Núñez, ConstanzaGarcia Otero, LauraRoca Agujetas, VicenteCatalán García, MarcGonzález Casacuberta, IngridTobías, EsterMilisenda, JoséGrau Junyent, Josep M. (Josep Maria)Crispi Brillas, FàtimaGratacós Solsona, EduardCardellach, FrancescGarrabou Tornos, GlòriaBioenergèticaMitocondrisEmbaràsBioenergeticsMitochondriaPregnancyIntrauterine growth restriction (IUGR) is an obstetric complication characterised by placental insufficiency and secondary cardiovascular remodelling that can lead to cardiomyopathy in adulthood. Despite its aetiology and potential therapeutics are poorly understood, bioenergetic deficits have been demonstrated in adverse foetal and cardiac development. We aimed to evaluate the role of mitochondria in human pregnancies with IUGR. In a single-site, cross-sectional and observational study, we included placenta and maternal peripheral and neonatal cord blood mononuclear cells (PBMC and CBMC) from 14 IUGR and 22 control pregnancies. The following mitochondrial measurements were assessed: enzymatic activities of mitochondrial respiratory chain (MRC) complexes I, II, IV, I + III and II + III, oxygen consumption (cell and complex I-stimulated respiration), mitochondrial content (citrate synthase [CS] activity and mitochondrial DNA copy number), total ATP levels and lipid peroxidation. Sirtuin3 expression was evaluated as a potential regulator of bioenergetic imbalance. Intrauterine growth restriction placental tissue showed a significant decrease of MRC CI enzymatic activity (P < 0.05) and CI-stimulated oxygen consumption (P < 0.05) accompanied by a significant increase of Sirtuin3/β-actin protein levels (P < 0.05). Maternal PBMC and neonatal CBMC from IUGR patients presented a not significant decrease in oxygen consumption (cell and CI-stimulated respiration) and MRC enzymatic activities (CII and CIV). Moreover, CS activity was significantly reduced in IUGR new-borns (P < 0.05). Total ATP levels and lipid peroxidation were preserved in all the studied tissues. Altered mitochondrial function of IUGR is especially present at placental and neonatal level, conveying potential targets to modulate obstetric outcome through dietary interventions aimed to regulate Sirtuin3 function.John Wiley & Sons2020202020192020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion12 p.application/pdfhttps://hdl.handle.net/2445/149180Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1111/jcmm.14282Journal of Cellular and Molecular Medicine, 2019, vol. 23, num. 6, p. 3962-3973https://doi.org/10.1111/jcmm.14282cc-by (c) Guitart Mampel, Mariona et al., 2019http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1491802026-05-29T05:05:01Z
dc.title.none.fl_str_mv Mitochondrial implications in human pregnancies with intrauterine growth restriction and associated cardiac remodelling
title Mitochondrial implications in human pregnancies with intrauterine growth restriction and associated cardiac remodelling
spellingShingle Mitochondrial implications in human pregnancies with intrauterine growth restriction and associated cardiac remodelling
Guitart Mampel, Mariona
Bioenergètica
Mitocondris
Embaràs
Bioenergetics
Mitochondria
Pregnancy
title_short Mitochondrial implications in human pregnancies with intrauterine growth restriction and associated cardiac remodelling
title_full Mitochondrial implications in human pregnancies with intrauterine growth restriction and associated cardiac remodelling
title_fullStr Mitochondrial implications in human pregnancies with intrauterine growth restriction and associated cardiac remodelling
title_full_unstemmed Mitochondrial implications in human pregnancies with intrauterine growth restriction and associated cardiac remodelling
title_sort Mitochondrial implications in human pregnancies with intrauterine growth restriction and associated cardiac remodelling
dc.creator.none.fl_str_mv Guitart Mampel, Mariona
Juárez Flores, Diana Luz
Youssef, Lina
Morén Núñez, Constanza
Garcia Otero, Laura
Roca Agujetas, Vicente
Catalán García, Marc
González Casacuberta, Ingrid
Tobías, Ester
Milisenda, José
Grau Junyent, Josep M. (Josep Maria)
Crispi Brillas, Fàtima
Gratacós Solsona, Eduard
Cardellach, Francesc
Garrabou Tornos, Glòria
author Guitart Mampel, Mariona
author_facet Guitart Mampel, Mariona
Juárez Flores, Diana Luz
Youssef, Lina
Morén Núñez, Constanza
Garcia Otero, Laura
Roca Agujetas, Vicente
Catalán García, Marc
González Casacuberta, Ingrid
Tobías, Ester
Milisenda, José
Grau Junyent, Josep M. (Josep Maria)
Crispi Brillas, Fàtima
Gratacós Solsona, Eduard
Cardellach, Francesc
Garrabou Tornos, Glòria
author_role author
author2 Juárez Flores, Diana Luz
Youssef, Lina
Morén Núñez, Constanza
Garcia Otero, Laura
Roca Agujetas, Vicente
Catalán García, Marc
González Casacuberta, Ingrid
Tobías, Ester
Milisenda, José
Grau Junyent, Josep M. (Josep Maria)
Crispi Brillas, Fàtima
Gratacós Solsona, Eduard
Cardellach, Francesc
Garrabou Tornos, Glòria
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bioenergètica
Mitocondris
Embaràs
Bioenergetics
Mitochondria
Pregnancy
topic Bioenergètica
Mitocondris
Embaràs
Bioenergetics
Mitochondria
Pregnancy
description Intrauterine growth restriction (IUGR) is an obstetric complication characterised by placental insufficiency and secondary cardiovascular remodelling that can lead to cardiomyopathy in adulthood. Despite its aetiology and potential therapeutics are poorly understood, bioenergetic deficits have been demonstrated in adverse foetal and cardiac development. We aimed to evaluate the role of mitochondria in human pregnancies with IUGR. In a single-site, cross-sectional and observational study, we included placenta and maternal peripheral and neonatal cord blood mononuclear cells (PBMC and CBMC) from 14 IUGR and 22 control pregnancies. The following mitochondrial measurements were assessed: enzymatic activities of mitochondrial respiratory chain (MRC) complexes I, II, IV, I + III and II + III, oxygen consumption (cell and complex I-stimulated respiration), mitochondrial content (citrate synthase [CS] activity and mitochondrial DNA copy number), total ATP levels and lipid peroxidation. Sirtuin3 expression was evaluated as a potential regulator of bioenergetic imbalance. Intrauterine growth restriction placental tissue showed a significant decrease of MRC CI enzymatic activity (P < 0.05) and CI-stimulated oxygen consumption (P < 0.05) accompanied by a significant increase of Sirtuin3/β-actin protein levels (P < 0.05). Maternal PBMC and neonatal CBMC from IUGR patients presented a not significant decrease in oxygen consumption (cell and CI-stimulated respiration) and MRC enzymatic activities (CII and CIV). Moreover, CS activity was significantly reduced in IUGR new-borns (P < 0.05). Total ATP levels and lipid peroxidation were preserved in all the studied tissues. Altered mitochondrial function of IUGR is especially present at placental and neonatal level, conveying potential targets to modulate obstetric outcome through dietary interventions aimed to regulate Sirtuin3 function.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/149180
url https://hdl.handle.net/2445/149180
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1111/jcmm.14282
Journal of Cellular and Molecular Medicine, 2019, vol. 23, num. 6, p. 3962-3973
https://doi.org/10.1111/jcmm.14282
dc.rights.none.fl_str_mv cc-by (c) Guitart Mampel, Mariona et al., 2019
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Guitart Mampel, Mariona et al., 2019
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12 p.
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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