High rates of intestinal bicarbonate secretion in seawater tilapia (Oreochromis mossambicus)

Osmoregulation in fish is a complex process that requires the orchestrated cooperation of many tissues. In fish facing hyperosmotic environments, the intestinal absorption of some monovalent ions and the secretion of bicarbonate are key processes to favor water absorption. In the present study, we s...

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Detalles Bibliográficos
Autores: Ruiz-Jarabo, Ignacio, Gregório, Silvia Filipa, Gaetano, Pasqualina, Trischitta, Francesca, Fuentes, Juan
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/343606
Acceso en línea:http://hdl.handle.net/10261/343606
https://api.elsevier.com/content/abstract/scopus_id/85013879029
Access Level:acceso abierto
Palabra clave:Osmoregulation
Bicarbonate secretion
Intestine
Ion transport
Oreochromis mossambicus
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spelling High rates of intestinal bicarbonate secretion in seawater tilapia (Oreochromis mossambicus)Ruiz-Jarabo, IgnacioGregório, Silvia FilipaGaetano, PasqualinaTrischitta, FrancescaFuentes, JuanOsmoregulationBicarbonate secretionIntestineIon transportOreochromis mossambicusOsmoregulation in fish is a complex process that requires the orchestrated cooperation of many tissues. In fish facing hyperosmotic environments, the intestinal absorption of some monovalent ions and the secretion of bicarbonate are key processes to favor water absorption. In the present study, we showed that bicarbonate levels in the intestinal fluid are several fold higher in seawater than in freshwater acclimated tilapia (Oreochromis mossambicus). In addition, we analyzed gene expression of the main molecular mechanisms involved in HCO3- movements i.e. slc26a6, slc26a3, slc4a4 and v-type H-ATPase sub C in the intestine of tilapia acclimated to both seawater and freshwater. Our results show an anterior/posterior functional regionalization of the intestine in tilapia in terms of expression patterns, which is affected by environmental salinity mostly in the anterior and mid intestine. Analysis of bicarbonate secretion using pH-Stat in tissues mounted in Ussing chambers reveals high rates of bicarbonate secretion in tilapia acclimated to seawater from anterior intestine to rectum ranging between ~900 and ~1700nmolHCO3-cm-2h-1. However, a relationship between the expression of slc26a6, slc26a3, slc4a4 and the rate of bicarbonate secretion seems to be compromised in the rectum. In this region, the low expression of the bicarbonate transporters could not explain the high bicarbonate secretion rates here described. However, we postulate that the elevated v-type H-ATPase mRNA expression in the rectum could be involved in this process.IR-J was funded by a postdoctoral grant (University of Cádiz, Spain). SFG was funded by the scholarship SFRH/BD/113363/2015 (FCT, Portugal). This work was supported by the Portuguese Foundation for Science and Technology (Ministry of Science and Higher Education, Portugal and European Social Funds) grant PTDC/MAR-BIO/3034/2014 to J.F. CCMar is supported by national funds from the Portuguese Foundation for Science and Technology (FCT) through project UID/Multi/04326/2013.Peer reviewedElsevierUniversidad de CádizFundação para a Ciência e a Tecnologia (Portugal)European CommissionMinistério da Ciência, Tecnologia e Ensino Superior (Portugal)202420242017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/343606https://api.elsevier.com/content/abstract/scopus_id/85013879029reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésComparative biochemistry and physiology. Part A, Molecular & integrative physiologyhttps://doi.org/10.1016/j.cbpa.2017.02.022Noinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3436062026-05-22T06:33:51Z
dc.title.none.fl_str_mv High rates of intestinal bicarbonate secretion in seawater tilapia (Oreochromis mossambicus)
title High rates of intestinal bicarbonate secretion in seawater tilapia (Oreochromis mossambicus)
spellingShingle High rates of intestinal bicarbonate secretion in seawater tilapia (Oreochromis mossambicus)
Ruiz-Jarabo, Ignacio
Osmoregulation
Bicarbonate secretion
Intestine
Ion transport
Oreochromis mossambicus
title_short High rates of intestinal bicarbonate secretion in seawater tilapia (Oreochromis mossambicus)
title_full High rates of intestinal bicarbonate secretion in seawater tilapia (Oreochromis mossambicus)
title_fullStr High rates of intestinal bicarbonate secretion in seawater tilapia (Oreochromis mossambicus)
title_full_unstemmed High rates of intestinal bicarbonate secretion in seawater tilapia (Oreochromis mossambicus)
title_sort High rates of intestinal bicarbonate secretion in seawater tilapia (Oreochromis mossambicus)
dc.creator.none.fl_str_mv Ruiz-Jarabo, Ignacio
Gregório, Silvia Filipa
Gaetano, Pasqualina
Trischitta, Francesca
Fuentes, Juan
author Ruiz-Jarabo, Ignacio
author_facet Ruiz-Jarabo, Ignacio
Gregório, Silvia Filipa
Gaetano, Pasqualina
Trischitta, Francesca
Fuentes, Juan
author_role author
author2 Gregório, Silvia Filipa
Gaetano, Pasqualina
Trischitta, Francesca
Fuentes, Juan
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidad de Cádiz
Fundação para a Ciência e a Tecnologia (Portugal)
European Commission
Ministério da Ciência, Tecnologia e Ensino Superior (Portugal)
dc.subject.none.fl_str_mv Osmoregulation
Bicarbonate secretion
Intestine
Ion transport
Oreochromis mossambicus
topic Osmoregulation
Bicarbonate secretion
Intestine
Ion transport
Oreochromis mossambicus
description Osmoregulation in fish is a complex process that requires the orchestrated cooperation of many tissues. In fish facing hyperosmotic environments, the intestinal absorption of some monovalent ions and the secretion of bicarbonate are key processes to favor water absorption. In the present study, we showed that bicarbonate levels in the intestinal fluid are several fold higher in seawater than in freshwater acclimated tilapia (Oreochromis mossambicus). In addition, we analyzed gene expression of the main molecular mechanisms involved in HCO3- movements i.e. slc26a6, slc26a3, slc4a4 and v-type H-ATPase sub C in the intestine of tilapia acclimated to both seawater and freshwater. Our results show an anterior/posterior functional regionalization of the intestine in tilapia in terms of expression patterns, which is affected by environmental salinity mostly in the anterior and mid intestine. Analysis of bicarbonate secretion using pH-Stat in tissues mounted in Ussing chambers reveals high rates of bicarbonate secretion in tilapia acclimated to seawater from anterior intestine to rectum ranging between ~900 and ~1700nmolHCO3-cm-2h-1. However, a relationship between the expression of slc26a6, slc26a3, slc4a4 and the rate of bicarbonate secretion seems to be compromised in the rectum. In this region, the low expression of the bicarbonate transporters could not explain the high bicarbonate secretion rates here described. However, we postulate that the elevated v-type H-ATPase mRNA expression in the rectum could be involved in this process.
publishDate 2017
dc.date.none.fl_str_mv 2017
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/343606
https://api.elsevier.com/content/abstract/scopus_id/85013879029
url http://hdl.handle.net/10261/343606
https://api.elsevier.com/content/abstract/scopus_id/85013879029
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
https://doi.org/10.1016/j.cbpa.2017.02.022
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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