Inflammatory Molecules in the Tears of Patients with Keratoconus

Purpose: To determine levels of a panel of inflammatory molecules and matrix metalloproteinases in the tears of patients with keratoconus. Design: A prospective, case-control study. Participants: Twenty-eight patients (1 eye from each) diagnosed with keratoconus at the Instituto Galego de Oftalmolox...

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Detalhes bibliográficos
Autores: Lema Gesto, María Isabel, Durán, Juan A.
Formato: artículo
Fecha de publicación:2005
País:España
Recursos:Universidad de Santiago de Compostela (USC)
Repositorio:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Idioma:inglés
OAI Identifier:oai:dnet:minerva_____::432f6ca3b28d60b6e461cc69edc0bff1
Acesso em linha:https://hdl.handle.net/10347/46718
Access Level:acceso abierto
Palavra-chave:Keratoconus
Interleukin-6
Enzyme-Linked Immunosorbent Assay
Matrix Metalloproteinase 9
32 Ciencias médicas
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spelling Inflammatory Molecules in the Tears of Patients with KeratoconusLema Gesto, María IsabelDurán, Juan A.KeratoconusInterleukin-6Enzyme-Linked Immunosorbent AssayMatrix Metalloproteinase 932 Ciencias médicasPurpose: To determine levels of a panel of inflammatory molecules and matrix metalloproteinases in the tears of patients with keratoconus. Design: A prospective, case-control study. Participants: Twenty-eight patients (1 eye from each) diagnosed with keratoconus at the Instituto Galego de Oftalmoloxía, Santiago de Compostela, Spain, during the period from September 2001 to June 2002, and 20 normal control subjects (1 eye each) were studied. Methods: Patients with keratoconus were examined in a routine fashion, and keratometric readings were taken to monitor the degree of ectasia. Fifteen microliters of tears was collected by capillary flow from each eye. Main Outcome Measures: The concentrations of cytokines (interleukin-4 [IL-4], IL-6, IL-10, and tumor necrosis factor α [TNF-α]), cell adhesion molecules (intercellular adhesion molecule 1 and vascular cell adhesion molecule 1), and matrix metalloproteinase 9 (MMP-9) were measured by enzyme-linked immunoadsorbent assay. Results: Patients with keratoconus initially had significantly higher levels of IL-6 (6.7 [4.8-10.8] pg/ml vs. 2.2 [1.0-4.1] pg/ml in control subjects [P<0.0001]), TNF-α (3.8 [2.9-14.4] pg/ml vs. 1.8 [1.5-2.3] pg/ml in control subjects [P<0.0001]), and MMP-9 (66.5 [49.2-139.3]ng/ml vs. 6.1 [3.9-8.3] ng/ml in control subjects. The extent of the increase was found to be associated with the severity of keratoconus. Conclusions: Interleukin-6, TNF-α, and MMP-9 are overexpressed in the tears of patients with keratoconus, indicating that the pathogenesis of keratoconus may involve chronic inflammatory events. © 2005 by the American Academy of Ophthalmology.American Academy of Ophthalmology | ElsevierUniversidade de Santiago de Compostela. Departamento de Cirurxía e Especialidades Médico-Cirúrxicas20052005-01-0120052005-01-01journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10347/46718reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:dnet:minerva_____::432f6ca3b28d60b6e461cc69edc0bff12026-06-15T12:47:27Z
dc.title.none.fl_str_mv Inflammatory Molecules in the Tears of Patients with Keratoconus
title Inflammatory Molecules in the Tears of Patients with Keratoconus
spellingShingle Inflammatory Molecules in the Tears of Patients with Keratoconus
Lema Gesto, María Isabel
Keratoconus
Interleukin-6
Enzyme-Linked Immunosorbent Assay
Matrix Metalloproteinase 9
32 Ciencias médicas
title_short Inflammatory Molecules in the Tears of Patients with Keratoconus
title_full Inflammatory Molecules in the Tears of Patients with Keratoconus
title_fullStr Inflammatory Molecules in the Tears of Patients with Keratoconus
title_full_unstemmed Inflammatory Molecules in the Tears of Patients with Keratoconus
title_sort Inflammatory Molecules in the Tears of Patients with Keratoconus
dc.creator.none.fl_str_mv Lema Gesto, María Isabel
Durán, Juan A.
author Lema Gesto, María Isabel
author_facet Lema Gesto, María Isabel
Durán, Juan A.
author_role author
author2 Durán, Juan A.
author2_role author
dc.contributor.none.fl_str_mv Universidade de Santiago de Compostela. Departamento de Cirurxía e Especialidades Médico-Cirúrxicas
dc.subject.none.fl_str_mv Keratoconus
Interleukin-6
Enzyme-Linked Immunosorbent Assay
Matrix Metalloproteinase 9
32 Ciencias médicas
topic Keratoconus
Interleukin-6
Enzyme-Linked Immunosorbent Assay
Matrix Metalloproteinase 9
32 Ciencias médicas
description Purpose: To determine levels of a panel of inflammatory molecules and matrix metalloproteinases in the tears of patients with keratoconus. Design: A prospective, case-control study. Participants: Twenty-eight patients (1 eye from each) diagnosed with keratoconus at the Instituto Galego de Oftalmoloxía, Santiago de Compostela, Spain, during the period from September 2001 to June 2002, and 20 normal control subjects (1 eye each) were studied. Methods: Patients with keratoconus were examined in a routine fashion, and keratometric readings were taken to monitor the degree of ectasia. Fifteen microliters of tears was collected by capillary flow from each eye. Main Outcome Measures: The concentrations of cytokines (interleukin-4 [IL-4], IL-6, IL-10, and tumor necrosis factor α [TNF-α]), cell adhesion molecules (intercellular adhesion molecule 1 and vascular cell adhesion molecule 1), and matrix metalloproteinase 9 (MMP-9) were measured by enzyme-linked immunoadsorbent assay. Results: Patients with keratoconus initially had significantly higher levels of IL-6 (6.7 [4.8-10.8] pg/ml vs. 2.2 [1.0-4.1] pg/ml in control subjects [P<0.0001]), TNF-α (3.8 [2.9-14.4] pg/ml vs. 1.8 [1.5-2.3] pg/ml in control subjects [P<0.0001]), and MMP-9 (66.5 [49.2-139.3]ng/ml vs. 6.1 [3.9-8.3] ng/ml in control subjects. The extent of the increase was found to be associated with the severity of keratoconus. Conclusions: Interleukin-6, TNF-α, and MMP-9 are overexpressed in the tears of patients with keratoconus, indicating that the pathogenesis of keratoconus may involve chronic inflammatory events. © 2005 by the American Academy of Ophthalmology.
publishDate 2005
dc.date.none.fl_str_mv 2005
2005-01-01
2005
2005-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10347/46718
url https://hdl.handle.net/10347/46718
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Academy of Ophthalmology | Elsevier
publisher.none.fl_str_mv American Academy of Ophthalmology | Elsevier
dc.source.none.fl_str_mv reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
instname:Universidad de Santiago de Compostela (USC)
instname_str Universidad de Santiago de Compostela (USC)
reponame_str Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
collection Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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