Nitric Oxide-Dependent LTD at Infralimbic Cortex

Dendritic calcium (Ca2+) spikes play a key role in the genesis of long-term synaptic plasticity. Although synaptic plasticity in the infralimbic cortex is critical for the extinction of fear-conditioned memory, the role of Ca2+-spikes in the induction of synaptic plasticity at this cortex has not be...

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Detalles Bibliográficos
Autores: Noriega Prieto, José Antonio, Maglio, Laura Eva, Gallero-Salas, Yasir, Fernández de Sevilla García, David
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/713518
Acceso en línea:http://hdl.handle.net/10486/713518
https://dx.doi.org/10.1016/j.neuroscience.2019.08.029
Access Level:acceso abierto
Palabra clave:calcium spikes
dendritic excitability
L-type VGCC
nitric oxide
prefrontal cortex
NMDARs
Medicina
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spelling Nitric Oxide-Dependent LTD at Infralimbic CortexNoriega Prieto, José AntonioMaglio, Laura EvaGallero-Salas, YasirFernández de Sevilla García, Davidcalcium spikesdendritic excitabilityL-type VGCCnitric oxideprefrontal cortexNMDARsMedicinaDendritic calcium (Ca2+) spikes play a key role in the genesis of long-term synaptic plasticity. Although synaptic plasticity in the infralimbic cortex is critical for the extinction of fear-conditioned memory, the role of Ca2+-spikes in the induction of synaptic plasticity at this cortex has not been explored in depth. Here we show that Ca2+-spikes in layer 5 pyramidal neurons (L5 PNs) of the rat infralimbic cortex are crucial in the induction of long-term depression of the excitatory postsynaptic currents (EPSCs). The lack of effect on the postsynaptic currents evoked by puffing glutamate and the changes in the variance of the EPSC amplitude that paralleled its inhibition suggest that this LTD of the EPSCs is mediated presynaptically. However, its induction requires cytosolic calcium elevations because it is prevented when the recorded L5 PN is loaded with BAPTA. Moreover, it depends on the synthesis of nitric oxide (NO) because it is absent on slices incubated with nitric oxidase synthase inhibitor L-NAME. Therefore, Ca2+-spikes can trigger LTD of the ESPCs through the NO dependent presynaptic form of synaptic plasticity, thus providing a novel form of inducing synaptic plasticity at L5 PNs of the rat infralimbic cortexThis work was supported by grants from Ministerio de Ciencia e Innovación, Spain (MICINN; BFU2011-23522) and Ministerio de Economía y Competitividad, Spain (MINECO; BFU2013-43668-P and BFU2016-80802-P AEI/FEDER, UE) to Dr. D. Fernández de Sevilla, professor at the Departamento de Anatomía, Histología y Neurociencia, Facultad de Medicina, UniversidadAutónoma de MadridElsevierDepartamento de Anatomía, Histología y NeurocienciaFacultad de Medicina20192019-10-15research articlehttp://purl.org/coar/resource_type/c_2df8fbb1AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/713518https://dx.doi.org/10.1016/j.neuroscience.2019.08.029reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7135182026-06-23T12:46:27Z
dc.title.none.fl_str_mv Nitric Oxide-Dependent LTD at Infralimbic Cortex
title Nitric Oxide-Dependent LTD at Infralimbic Cortex
spellingShingle Nitric Oxide-Dependent LTD at Infralimbic Cortex
Noriega Prieto, José Antonio
calcium spikes
dendritic excitability
L-type VGCC
nitric oxide
prefrontal cortex
NMDARs
Medicina
title_short Nitric Oxide-Dependent LTD at Infralimbic Cortex
title_full Nitric Oxide-Dependent LTD at Infralimbic Cortex
title_fullStr Nitric Oxide-Dependent LTD at Infralimbic Cortex
title_full_unstemmed Nitric Oxide-Dependent LTD at Infralimbic Cortex
title_sort Nitric Oxide-Dependent LTD at Infralimbic Cortex
dc.creator.none.fl_str_mv Noriega Prieto, José Antonio
Maglio, Laura Eva
Gallero-Salas, Yasir
Fernández de Sevilla García, David
author Noriega Prieto, José Antonio
author_facet Noriega Prieto, José Antonio
Maglio, Laura Eva
Gallero-Salas, Yasir
Fernández de Sevilla García, David
author_role author
author2 Maglio, Laura Eva
Gallero-Salas, Yasir
Fernández de Sevilla García, David
author2_role author
author
author
dc.contributor.none.fl_str_mv Departamento de Anatomía, Histología y Neurociencia
Facultad de Medicina
dc.subject.none.fl_str_mv calcium spikes
dendritic excitability
L-type VGCC
nitric oxide
prefrontal cortex
NMDARs
Medicina
topic calcium spikes
dendritic excitability
L-type VGCC
nitric oxide
prefrontal cortex
NMDARs
Medicina
description Dendritic calcium (Ca2+) spikes play a key role in the genesis of long-term synaptic plasticity. Although synaptic plasticity in the infralimbic cortex is critical for the extinction of fear-conditioned memory, the role of Ca2+-spikes in the induction of synaptic plasticity at this cortex has not been explored in depth. Here we show that Ca2+-spikes in layer 5 pyramidal neurons (L5 PNs) of the rat infralimbic cortex are crucial in the induction of long-term depression of the excitatory postsynaptic currents (EPSCs). The lack of effect on the postsynaptic currents evoked by puffing glutamate and the changes in the variance of the EPSC amplitude that paralleled its inhibition suggest that this LTD of the EPSCs is mediated presynaptically. However, its induction requires cytosolic calcium elevations because it is prevented when the recorded L5 PN is loaded with BAPTA. Moreover, it depends on the synthesis of nitric oxide (NO) because it is absent on slices incubated with nitric oxidase synthase inhibitor L-NAME. Therefore, Ca2+-spikes can trigger LTD of the ESPCs through the NO dependent presynaptic form of synaptic plasticity, thus providing a novel form of inducing synaptic plasticity at L5 PNs of the rat infralimbic cortex
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-10-15
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/713518
https://dx.doi.org/10.1016/j.neuroscience.2019.08.029
url http://hdl.handle.net/10486/713518
https://dx.doi.org/10.1016/j.neuroscience.2019.08.029
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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