Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius

In microorganisms, Ion Torrent sequencing technology has been proved to be useful in whole-genome sequencing of bacterial genomes (5 Mbp). In our study, for the first time we used this technology to perform a resequencing approach in a whole fungal genome (36 Mbp), a non-ochratoxin A producing strai...

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Autores: Cabañes Sáenz, Francisco Javier|||0000-0002-2244-5778, Sanseverino, Walter|||0000-0003-3324-5912, Castellá, Gemma|||0000-0002-8404-0688, Bragulat, M. Rosa|||0000-0003-1853-1777, Aiese Cigliano, Riccardo|||0000-0002-9058-6994, Sánchez Bonastre, Armando|||0000-0001-9160-1124
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:203836
Acceso en línea:https://ddd.uab.cat/record/203836
https://dx.doi.org/urn:doi:10.1038/srep09086
Access Level:acceso abierto
Palabra clave:Aspergillus
Computational Biology
DNA Copy Number Variations
Genes, Fungal
Genome, Fungal
Genomics
Molecular Sequence Annotation
Phylogeny
Polymorphism, Single Nucleotide
Sequence Analysis, DNA
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spelling Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonariusCabañes Sáenz, Francisco Javier|||0000-0002-2244-5778Sanseverino, Walter|||0000-0003-3324-5912Castellá, Gemma|||0000-0002-8404-0688Bragulat, M. Rosa|||0000-0003-1853-1777Aiese Cigliano, Riccardo|||0000-0002-9058-6994Sánchez Bonastre, Armando|||0000-0001-9160-1124AspergillusComputational BiologyDNA Copy Number VariationsGenes, FungalGenome, FungalGenomicsMolecular Sequence AnnotationPhylogenyPolymorphism, Single NucleotideSequence Analysis, DNAIn microorganisms, Ion Torrent sequencing technology has been proved to be useful in whole-genome sequencing of bacterial genomes (5 Mbp). In our study, for the first time we used this technology to perform a resequencing approach in a whole fungal genome (36 Mbp), a non-ochratoxin A producing strain of Aspergillus carbonarius. Ochratoxin A (OTA) is a potent nephrotoxin which is found mainly in cereals and their products, but it also occurs in a variety of common foods and beverages. Due to the fact that this strain does not produce OTA, we focused some of the bioinformatics analyses in genes involved in OTA biosynthesis, using a reference genome of an OTA producing strain of the same species. This study revealed that in the atoxigenic strain there is a high accumulation of nonsense and missense mutations in several genes. Importantly, a two fold increase in gene mutation ratio was observed in PKS and NRPS encoding genes which are suggested to be involved in OTA biosynthesis. 22015-01-0120152015-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/203836https://dx.doi.org/urn:doi:10.1038/srep09086reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Ciencia e Innovación https://doi.org/10.13039/501100004837 AGL2010-22182-C04-02open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2038362026-06-06T12:50:31Z
dc.title.none.fl_str_mv Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius
title Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius
spellingShingle Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius
Cabañes Sáenz, Francisco Javier|||0000-0002-2244-5778
Aspergillus
Computational Biology
DNA Copy Number Variations
Genes, Fungal
Genome, Fungal
Genomics
Molecular Sequence Annotation
Phylogeny
Polymorphism, Single Nucleotide
Sequence Analysis, DNA
title_short Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius
title_full Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius
title_fullStr Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius
title_full_unstemmed Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius
title_sort Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius
dc.creator.none.fl_str_mv Cabañes Sáenz, Francisco Javier|||0000-0002-2244-5778
Sanseverino, Walter|||0000-0003-3324-5912
Castellá, Gemma|||0000-0002-8404-0688
Bragulat, M. Rosa|||0000-0003-1853-1777
Aiese Cigliano, Riccardo|||0000-0002-9058-6994
Sánchez Bonastre, Armando|||0000-0001-9160-1124
author Cabañes Sáenz, Francisco Javier|||0000-0002-2244-5778
author_facet Cabañes Sáenz, Francisco Javier|||0000-0002-2244-5778
Sanseverino, Walter|||0000-0003-3324-5912
Castellá, Gemma|||0000-0002-8404-0688
Bragulat, M. Rosa|||0000-0003-1853-1777
Aiese Cigliano, Riccardo|||0000-0002-9058-6994
Sánchez Bonastre, Armando|||0000-0001-9160-1124
author_role author
author2 Sanseverino, Walter|||0000-0003-3324-5912
Castellá, Gemma|||0000-0002-8404-0688
Bragulat, M. Rosa|||0000-0003-1853-1777
Aiese Cigliano, Riccardo|||0000-0002-9058-6994
Sánchez Bonastre, Armando|||0000-0001-9160-1124
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Aspergillus
Computational Biology
DNA Copy Number Variations
Genes, Fungal
Genome, Fungal
Genomics
Molecular Sequence Annotation
Phylogeny
Polymorphism, Single Nucleotide
Sequence Analysis, DNA
topic Aspergillus
Computational Biology
DNA Copy Number Variations
Genes, Fungal
Genome, Fungal
Genomics
Molecular Sequence Annotation
Phylogeny
Polymorphism, Single Nucleotide
Sequence Analysis, DNA
description In microorganisms, Ion Torrent sequencing technology has been proved to be useful in whole-genome sequencing of bacterial genomes (5 Mbp). In our study, for the first time we used this technology to perform a resequencing approach in a whole fungal genome (36 Mbp), a non-ochratoxin A producing strain of Aspergillus carbonarius. Ochratoxin A (OTA) is a potent nephrotoxin which is found mainly in cereals and their products, but it also occurs in a variety of common foods and beverages. Due to the fact that this strain does not produce OTA, we focused some of the bioinformatics analyses in genes involved in OTA biosynthesis, using a reference genome of an OTA producing strain of the same species. This study revealed that in the atoxigenic strain there is a high accumulation of nonsense and missense mutations in several genes. Importantly, a two fold increase in gene mutation ratio was observed in PKS and NRPS encoding genes which are suggested to be involved in OTA biosynthesis.
publishDate 2015
dc.date.none.fl_str_mv 2
2015-01-01
2015
2015-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/203836
https://dx.doi.org/urn:doi:10.1038/srep09086
url https://ddd.uab.cat/record/203836
https://dx.doi.org/urn:doi:10.1038/srep09086
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 AGL2010-22182-C04-02
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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