Replication fork barriers and topological barriers: progression of DNA replication relies on DNA topology ahead of forks

22 p.-5 fig.

Detalles Bibliográficos
Autores: Schvartzman, Jorge Bernardo, Hernandez, Pablo, Krimer, Dora B.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/203192
Acceso en línea:http://hdl.handle.net/10261/203192
Access Level:acceso abierto
Palabra clave:DNA topology
Replication
Supercoiling
Pre-catenation
Two-dimensional agarose gel electrophoresis
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spelling Replication fork barriers and topological barriers: progression of DNA replication relies on DNA topology ahead of forksRunning Title: DNA replication and topologySchvartzman, Jorge BernardoHernandez, PabloKrimer, Dora B.DNA topologyReplicationSupercoilingPre-catenationTwo-dimensional agarose gel electrophoresis22 p.-5 fig.During replication, the topology of DNA changes continuously in response to well‐known activities of DNA helicases, polymerases, and topoisomerases. However, replisomes do not always progress at a constant speed and can slow‐down and even stall at precise sites. The way these changes in the rate of replisome progression affect DNA topology is not yet well understood. The interplay of DNA topology and replication in several cases where progression of replication forks reacts differently to changes in DNA topology ahead is discussed here. It is proposed, there are at least two types of replication fork barriers: those that behave also as topological barriers and those that do not. Two‐Dimensional (2D) agarose gel electrophoresis is the method of choice to distinguish between these two different types of replication fork barriers.Spanish Ministerio de Economía y Competitividad. Grant Number: BFU2014‐56835Peer reviewedJohn Wiley & SonsMinisterio de Economía y Competitividad (España)Hernandez, Pablo [0000-0002-8113-1277]Krimer, Dora B. [0000-0001-9972-3994]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/203192reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2014-56835-Phttps://doi.org/10.1002/bies.201900204Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2031922026-05-22T06:33:51Z
dc.title.none.fl_str_mv Replication fork barriers and topological barriers: progression of DNA replication relies on DNA topology ahead of forks
Running Title: DNA replication and topology
title Replication fork barriers and topological barriers: progression of DNA replication relies on DNA topology ahead of forks
spellingShingle Replication fork barriers and topological barriers: progression of DNA replication relies on DNA topology ahead of forks
Schvartzman, Jorge Bernardo
DNA topology
Replication
Supercoiling
Pre-catenation
Two-dimensional agarose gel electrophoresis
title_short Replication fork barriers and topological barriers: progression of DNA replication relies on DNA topology ahead of forks
title_full Replication fork barriers and topological barriers: progression of DNA replication relies on DNA topology ahead of forks
title_fullStr Replication fork barriers and topological barriers: progression of DNA replication relies on DNA topology ahead of forks
title_full_unstemmed Replication fork barriers and topological barriers: progression of DNA replication relies on DNA topology ahead of forks
title_sort Replication fork barriers and topological barriers: progression of DNA replication relies on DNA topology ahead of forks
dc.creator.none.fl_str_mv Schvartzman, Jorge Bernardo
Hernandez, Pablo
Krimer, Dora B.
author Schvartzman, Jorge Bernardo
author_facet Schvartzman, Jorge Bernardo
Hernandez, Pablo
Krimer, Dora B.
author_role author
author2 Hernandez, Pablo
Krimer, Dora B.
author2_role author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Hernandez, Pablo [0000-0002-8113-1277]
Krimer, Dora B. [0000-0001-9972-3994]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv DNA topology
Replication
Supercoiling
Pre-catenation
Two-dimensional agarose gel electrophoresis
topic DNA topology
Replication
Supercoiling
Pre-catenation
Two-dimensional agarose gel electrophoresis
description 22 p.-5 fig.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/203192
url http://hdl.handle.net/10261/203192
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2014-56835-P
https://doi.org/10.1002/bies.201900204
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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