Detection of Tuber melanosporum DNA in soil

Our objectives were (i) to develop a molecular method to detect mycelia of Tuber melanosporum (black truffle) in soil and (ii) to test for mycelial distribution around two truffle-bearing Quercus ilex trees in a truffle orchard. Isolation of total DNA from soil was performed, followed by PCR amplifi...

Descripción completa

Detalles Bibliográficos
Autores: Suz, Laura M., Martín, Laura P., Colinas, C. (Carlos)
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2006
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/57131
Acceso en línea:https://doi.org/10.1111/j.1574-6968.2005.00029.x
http://hdl.handle.net/10459.1/57131
Access Level:acceso abierto
Palabra clave:Tuber melanosporum
PCR-RFLP
Soil mycelium
Truffle orchard
id ES_ebc0bd31ee2f480b40779c4eec2f3fe4
oai_identifier_str oai:repositori.udl.cat:10459.1/57131
network_acronym_str ES
network_name_str España
repository_id_str
spelling Detection of Tuber melanosporum DNA in soilSuz, Laura M.Martín, Laura P.Colinas, C. (Carlos)Tuber melanosporumPCR-RFLPSoil myceliumTruffle orchardOur objectives were (i) to develop a molecular method to detect mycelia of Tuber melanosporum (black truffle) in soil and (ii) to test for mycelial distribution around two truffle-bearing Quercus ilex trees in a truffle orchard. Isolation of total DNA from soil was performed, followed by PCR amplification with T. melanosporum-specific primers and restriction analysis. To address the detection sensitivity level, soil samples were inoculated with known amounts of gleba of T. melanosporum. The detection limit was Z11.4 mg of hyphae g 1 of soil. Mycelium was detected primarily within the area defined by the truffle burn and within the top 35 cm of the soil in all directions from the trees.Oxford University Press2006info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttps://doi.org/10.1111/j.1574-6968.2005.00029.xhttp://hdl.handle.net/10459.1/57131reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)InglésVersió postprint del document publicat a https://doi.org/10.1111/j.1574-6968.2005.00029.xFEMS Microbiology Letters, 2006, vol. 254, núm. 2, p. 251-257(c) Federation of European Microbiological Societies, 2006info:eu-repo/semantics/openAccessoai:repositori.udl.cat:10459.1/571312026-06-24T12:42:17Z
dc.title.none.fl_str_mv Detection of Tuber melanosporum DNA in soil
title Detection of Tuber melanosporum DNA in soil
spellingShingle Detection of Tuber melanosporum DNA in soil
Suz, Laura M.
Tuber melanosporum
PCR-RFLP
Soil mycelium
Truffle orchard
title_short Detection of Tuber melanosporum DNA in soil
title_full Detection of Tuber melanosporum DNA in soil
title_fullStr Detection of Tuber melanosporum DNA in soil
title_full_unstemmed Detection of Tuber melanosporum DNA in soil
title_sort Detection of Tuber melanosporum DNA in soil
dc.creator.none.fl_str_mv Suz, Laura M.
Martín, Laura P.
Colinas, C. (Carlos)
author Suz, Laura M.
author_facet Suz, Laura M.
Martín, Laura P.
Colinas, C. (Carlos)
author_role author
author2 Martín, Laura P.
Colinas, C. (Carlos)
author2_role author
author
dc.subject.none.fl_str_mv Tuber melanosporum
PCR-RFLP
Soil mycelium
Truffle orchard
topic Tuber melanosporum
PCR-RFLP
Soil mycelium
Truffle orchard
description Our objectives were (i) to develop a molecular method to detect mycelia of Tuber melanosporum (black truffle) in soil and (ii) to test for mycelial distribution around two truffle-bearing Quercus ilex trees in a truffle orchard. Isolation of total DNA from soil was performed, followed by PCR amplification with T. melanosporum-specific primers and restriction analysis. To address the detection sensitivity level, soil samples were inoculated with known amounts of gleba of T. melanosporum. The detection limit was Z11.4 mg of hyphae g 1 of soil. Mycelium was detected primarily within the area defined by the truffle burn and within the top 35 cm of the soil in all directions from the trees.
publishDate 2006
dc.date.none.fl_str_mv 2006
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1111/j.1574-6968.2005.00029.x
http://hdl.handle.net/10459.1/57131
url https://doi.org/10.1111/j.1574-6968.2005.00029.x
http://hdl.handle.net/10459.1/57131
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a https://doi.org/10.1111/j.1574-6968.2005.00029.x
FEMS Microbiology Letters, 2006, vol. 254, núm. 2, p. 251-257
dc.rights.none.fl_str_mv (c) Federation of European Microbiological Societies, 2006
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Federation of European Microbiological Societies, 2006
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869423255064936448
score 15.81155

Similares en LA Referencia