Novel culture chamber to evaluate in vitro plant-microbe volatile interactions: effects of Trichoderma harzianum volatiles on wheat plantlets

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Detalles Bibliográficos
Autores: Álvarez García, Samuel, Manga Robles, Alba, Encina García, Antonio Esteban, Gutiérrez , Santiago 1965-, Casquero Luelmo, Pedro Antonio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universidad Rey Juan Carlos
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/14757
Acceso en línea:https://www.sciencedirect.com/science/article/pii/S0168945222001108?via%3Dihub
http://hdl.handle.net/10612/14757
Access Level:acceso abierto
Palabra clave:Fisiología
BVOC
Plant-microbe VOC Chamber
Trichoderma
Trichodiene
Volatile Organic Compounds
Wheat
2417.19 Fisiología Vegetal
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repository_id_str
spelling Novel culture chamber to evaluate in vitro plant-microbe volatile interactions: effects of Trichoderma harzianum volatiles on wheat plantletsÁlvarez García, SamuelManga Robles, AlbaEncina García, Antonio EstebanGutiérrez , Santiago 1965-Casquero Luelmo, Pedro AntonioFisiologíaBVOCPlant-microbe VOC ChamberTrichodermaTrichodieneVolatile Organic CompoundsWheat2417.19 Fisiología Vegetal.The field of plant-microbe interactions mediated by Biogenic Volatile Organic Compounds (BVOCs) still faces several limitations due to the lack of reliable equipment. We present a novel device designed to evaluate in vitro plant-microbe volatile interactions, the plant-microbe VOC Chamber. It was tested by evaluating the effects exerted on wheat development by volatiles from three Trichoderma harzianum strains, a wild type and two genetically modified strains; one expressing the tri5 gene, which leads to the synthesis and emission of the volatile trichodiene, and the other by silencing the erg1 gene, impairing ergosterol production. The wild type and the erg1-silenced strain enhanced fresh weight and length of the aerial part, but reduced root dry weight. Interestingly, no differences were found between them. Conversely, the tri5-transformant strain reduced root and aerial growth compared to the control and the other strains. No differences were observed regarding chlorophyll fluorescence quantum yield and leaf chlorophyll content, suggesting that the released BVOCs do not interfere with photosynthesis. The plant-microbe VOC Chamber proved to be a simple and reliable method to evaluate the in vitro effects of microbial BVOCs on plant development, perfect for the screening of microorganisms with interesting volatile traits. Availability of data and materials The data that support the findings of this study are available from the corresponding authors (Antonio Encina and Samuel Álvarez-García) upon reasonable request. All microbiological strains used in this study will be made available to researchers upon reasonable request. VOC Chambers will be made available to researchers upon reasonable request, unless commercial agreements reached with third parties regarding the patent exploitation prohibit it (in which case the VOC Chambers should be available in the market).SIElsevierFisiologia VegetalEscuela de Ingeniería Agraria y Forestal2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://www.sciencedirect.com/science/article/pii/S0168945222001108?via%3Dihubhttp://hdl.handle.net/10612/14757reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad Rey Juan CarlosInglésLE251P18,ORDEN EDU/529/2017, June 26th,ORDEN EDU/601/2020, july 7th,ULE-PoC, via FGULEMhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/147572026-06-24T12:43:27Z
dc.title.none.fl_str_mv Novel culture chamber to evaluate in vitro plant-microbe volatile interactions: effects of Trichoderma harzianum volatiles on wheat plantlets
title Novel culture chamber to evaluate in vitro plant-microbe volatile interactions: effects of Trichoderma harzianum volatiles on wheat plantlets
spellingShingle Novel culture chamber to evaluate in vitro plant-microbe volatile interactions: effects of Trichoderma harzianum volatiles on wheat plantlets
Álvarez García, Samuel
Fisiología
BVOC
Plant-microbe VOC Chamber
Trichoderma
Trichodiene
Volatile Organic Compounds
Wheat
2417.19 Fisiología Vegetal
title_short Novel culture chamber to evaluate in vitro plant-microbe volatile interactions: effects of Trichoderma harzianum volatiles on wheat plantlets
title_full Novel culture chamber to evaluate in vitro plant-microbe volatile interactions: effects of Trichoderma harzianum volatiles on wheat plantlets
title_fullStr Novel culture chamber to evaluate in vitro plant-microbe volatile interactions: effects of Trichoderma harzianum volatiles on wheat plantlets
title_full_unstemmed Novel culture chamber to evaluate in vitro plant-microbe volatile interactions: effects of Trichoderma harzianum volatiles on wheat plantlets
title_sort Novel culture chamber to evaluate in vitro plant-microbe volatile interactions: effects of Trichoderma harzianum volatiles on wheat plantlets
dc.creator.none.fl_str_mv Álvarez García, Samuel
Manga Robles, Alba
Encina García, Antonio Esteban
Gutiérrez , Santiago 1965-
Casquero Luelmo, Pedro Antonio
author Álvarez García, Samuel
author_facet Álvarez García, Samuel
Manga Robles, Alba
Encina García, Antonio Esteban
Gutiérrez , Santiago 1965-
Casquero Luelmo, Pedro Antonio
author_role author
author2 Manga Robles, Alba
Encina García, Antonio Esteban
Gutiérrez , Santiago 1965-
Casquero Luelmo, Pedro Antonio
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Fisiologia Vegetal
Escuela de Ingeniería Agraria y Forestal
dc.subject.none.fl_str_mv Fisiología
BVOC
Plant-microbe VOC Chamber
Trichoderma
Trichodiene
Volatile Organic Compounds
Wheat
2417.19 Fisiología Vegetal
topic Fisiología
BVOC
Plant-microbe VOC Chamber
Trichoderma
Trichodiene
Volatile Organic Compounds
Wheat
2417.19 Fisiología Vegetal
description .
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0168945222001108?via%3Dihub
http://hdl.handle.net/10612/14757
url https://www.sciencedirect.com/science/article/pii/S0168945222001108?via%3Dihub
http://hdl.handle.net/10612/14757
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv LE251P18,ORDEN EDU/529/2017, June 26th,ORDEN EDU/601/2020, july 7th,ULE-PoC, via FGULEM
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad Rey Juan Carlos
instname_str Universidad Rey Juan Carlos
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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