Characterization of hepatitis B virus X gene quasispecies complexity in mono-infection and hepatitis delta virus superinfection

Hepatitis B X gene; Hepatitis B virus; Hepatitis delta virus

Detalles Bibliográficos
Autores: Godoy Cruz, Cristina, Tabernero Caellas, David, Sopena Santisteve, Sara, Gonzalez Fernandez, Carolina, Casillas Gomez, Rosario, Yll Pico, Marçal, Lopez Martinez, Rosa, Rodríguez Frias, Francisco, Rando Segura, Ariadna, Gregori Font, Josep, Cortese, Maria Francesca
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:11351/4138
Acceso en línea:https://hdl.handle.net/11351/4138
http://hdl.handle.net/11351/4138
Access Level:acceso abierto
Palabra clave:Virus de l'hepatitis B
Hepatitis vírica - Aspectes genètics
ORGANISMS::Viruses::DNA Viruses::Hepadnaviridae::Orthohepadnavirus::Hepatitis B virus
ORGANISMS::Viruses::Defective Viruses::Hepatitis Delta Virus
Other subheadings::Other subheadings::Other subheadings::/genetics
PHENOMENA AND PROCESSES::Genetic Phenomena::Genetic Variation::Quasispecies
ORGANISMOS::virus::virus ADN::Hepadnaviridae::Orthohepadnavirus::virus de la hepatitis B
ORGANISMOS::virus::virus defectuosos::virus de la hepatitis delta
Otros calificadores::Otros calificadores::Otros calificadores::/genética
FENÓMENOS Y PROCESOS::fenómenos genéticos::variación genética::cuasiespecies
id ES_ebbf7ceca3cb610c6af0b89fc93773a4
oai_identifier_str oai:recercat.cat:11351/4138
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Characterization of hepatitis B virus X gene quasispecies complexity in mono-infection and hepatitis delta virus superinfection
title Characterization of hepatitis B virus X gene quasispecies complexity in mono-infection and hepatitis delta virus superinfection
spellingShingle Characterization of hepatitis B virus X gene quasispecies complexity in mono-infection and hepatitis delta virus superinfection
Godoy Cruz, Cristina
Virus de l'hepatitis B
Hepatitis vírica - Aspectes genètics
ORGANISMS::Viruses::DNA Viruses::Hepadnaviridae::Orthohepadnavirus::Hepatitis B virus
ORGANISMS::Viruses::Defective Viruses::Hepatitis Delta Virus
Other subheadings::Other subheadings::Other subheadings::/genetics
PHENOMENA AND PROCESSES::Genetic Phenomena::Genetic Variation::Quasispecies
ORGANISMOS::virus::virus ADN::Hepadnaviridae::Orthohepadnavirus::virus de la hepatitis B
ORGANISMOS::virus::virus defectuosos::virus de la hepatitis delta
Otros calificadores::Otros calificadores::Otros calificadores::/genética
FENÓMENOS Y PROCESOS::fenómenos genéticos::variación genética::cuasiespecies
title_short Characterization of hepatitis B virus X gene quasispecies complexity in mono-infection and hepatitis delta virus superinfection
title_full Characterization of hepatitis B virus X gene quasispecies complexity in mono-infection and hepatitis delta virus superinfection
title_fullStr Characterization of hepatitis B virus X gene quasispecies complexity in mono-infection and hepatitis delta virus superinfection
title_full_unstemmed Characterization of hepatitis B virus X gene quasispecies complexity in mono-infection and hepatitis delta virus superinfection
title_sort Characterization of hepatitis B virus X gene quasispecies complexity in mono-infection and hepatitis delta virus superinfection
dc.creator.none.fl_str_mv Godoy Cruz, Cristina
Tabernero Caellas, David
Sopena Santisteve, Sara
Gonzalez Fernandez, Carolina
Casillas Gomez, Rosario
Yll Pico, Marçal
Lopez Martinez, Rosa
Rodríguez Frias, Francisco
Rando Segura, Ariadna
Gregori Font, Josep
Cortese, Maria Francesca
author Godoy Cruz, Cristina
author_facet Godoy Cruz, Cristina
Tabernero Caellas, David
Sopena Santisteve, Sara
Gonzalez Fernandez, Carolina
Casillas Gomez, Rosario
Yll Pico, Marçal
Lopez Martinez, Rosa
Rodríguez Frias, Francisco
Rando Segura, Ariadna
Gregori Font, Josep
Cortese, Maria Francesca
author_role author
author2 Tabernero Caellas, David
Sopena Santisteve, Sara
Gonzalez Fernandez, Carolina
Casillas Gomez, Rosario
Yll Pico, Marçal
Lopez Martinez, Rosa
Rodríguez Frias, Francisco
Rando Segura, Ariadna
Gregori Font, Josep
Cortese, Maria Francesca
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Institut Català de la Salut
[Godoy C, Tabernero D, Sopena S, Cortese MF, González C, Casillas R, Yll M, Rando A, López-Martínez R, Rodríguez-Frías F] Unitat de patologia hepàtica, Servei de Bioquímica i Microbiologia, Hospital Universitari Vall d'Hebron, Barcelona, Spain. Universitat Autònoma de Barcelona, Barcelona, Spain. [Gregori J] Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, Madrid, Spain.
Vall d'Hebron Barcelona Hospital Campus
dc.subject.none.fl_str_mv Virus de l'hepatitis B
Hepatitis vírica - Aspectes genètics
ORGANISMS::Viruses::DNA Viruses::Hepadnaviridae::Orthohepadnavirus::Hepatitis B virus
ORGANISMS::Viruses::Defective Viruses::Hepatitis Delta Virus
Other subheadings::Other subheadings::Other subheadings::/genetics
PHENOMENA AND PROCESSES::Genetic Phenomena::Genetic Variation::Quasispecies
ORGANISMOS::virus::virus ADN::Hepadnaviridae::Orthohepadnavirus::virus de la hepatitis B
ORGANISMOS::virus::virus defectuosos::virus de la hepatitis delta
Otros calificadores::Otros calificadores::Otros calificadores::/genética
FENÓMENOS Y PROCESOS::fenómenos genéticos::variación genética::cuasiespecies
topic Virus de l'hepatitis B
Hepatitis vírica - Aspectes genètics
ORGANISMS::Viruses::DNA Viruses::Hepadnaviridae::Orthohepadnavirus::Hepatitis B virus
ORGANISMS::Viruses::Defective Viruses::Hepatitis Delta Virus
Other subheadings::Other subheadings::Other subheadings::/genetics
PHENOMENA AND PROCESSES::Genetic Phenomena::Genetic Variation::Quasispecies
ORGANISMOS::virus::virus ADN::Hepadnaviridae::Orthohepadnavirus::virus de la hepatitis B
ORGANISMOS::virus::virus defectuosos::virus de la hepatitis delta
Otros calificadores::Otros calificadores::Otros calificadores::/genética
FENÓMENOS Y PROCESOS::fenómenos genéticos::variación genética::cuasiespecies
description Hepatitis B X gene; Hepatitis B virus; Hepatitis delta virus
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11351/4138
http://hdl.handle.net/11351/4138
url https://hdl.handle.net/11351/4138
http://hdl.handle.net/11351/4138
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv World Journal of Gastroenterology;25(13)
https://www.wjgnet.com/1007-9327/full/v25/i13/1566.htm
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Baishideng Publishing Group
publisher.none.fl_str_mv Baishideng Publishing Group
dc.source.none.fl_str_mv Scientia
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869423254498705408
spelling Characterization of hepatitis B virus X gene quasispecies complexity in mono-infection and hepatitis delta virus superinfectionGodoy Cruz, CristinaTabernero Caellas, DavidSopena Santisteve, SaraGonzalez Fernandez, CarolinaCasillas Gomez, RosarioYll Pico, MarçalLopez Martinez, RosaRodríguez Frias, FranciscoRando Segura, AriadnaGregori Font, JosepCortese, Maria FrancescaVirus de l'hepatitis BHepatitis vírica - Aspectes genèticsORGANISMS::Viruses::DNA Viruses::Hepadnaviridae::Orthohepadnavirus::Hepatitis B virusORGANISMS::Viruses::Defective Viruses::Hepatitis Delta VirusOther subheadings::Other subheadings::Other subheadings::/geneticsPHENOMENA AND PROCESSES::Genetic Phenomena::Genetic Variation::QuasispeciesORGANISMOS::virus::virus ADN::Hepadnaviridae::Orthohepadnavirus::virus de la hepatitis BORGANISMOS::virus::virus defectuosos::virus de la hepatitis deltaOtros calificadores::Otros calificadores::Otros calificadores::/genéticaFENÓMENOS Y PROCESOS::fenómenos genéticos::variación genética::cuasiespeciesHepatitis B X gene; Hepatitis B virus; Hepatitis delta virusHepatitis B gen X; Virus d'hepatitis B; Virus d'hepatitis deltaHepatitis B gen X; Virus de hepatitis B; Virus de hepatitis deltaBACKGROUND: Hepatitis delta virus (HDV) seems to strongly suppress hepatitis B virus (HBV) replication, although little is known about the mechanism of this interaction. Both these viruses show a dynamic distribution of mutants, resulting in viral quasispecies. Next-generation sequencing is a viable approach for analyzing the composition of these mutant spectra. As the regulatory hepatitis B X protein (HBx) is essential for HBV replication, determination of HBV X gene (HBX) quasispecies complexity in HBV/HDV infection compared to HBV mono-infection may provide information on the interactions between these two viruses. AIM: To compare HBV quasispecies complexity in the HBX 5' region between chronic hepatitis delta (CHD) and chronic HBV mono-infected patients. METHODS: Twenty-four untreated patients were included: 7/24 (29.2%) with HBeAg-negative chronic HBV infection (CI, previously termed inactive carriers), 8/24 (33.3%) with HBeAg-negative chronic hepatitis B (CHB) and 9/24 (37.5%) with CHD. A serum sample from each patient was first tested for HBV DNA levels. The HBX 5' region [nucleotides (nt) 1255-1611] was then PCR-amplified for subsequent next-generation sequencing (MiSeq, Illumina, United States). HBV quasispecies complexity in the region analyzed was evaluated using incidence-based indices (number of haplotypes and number of mutations), abundance-based indices (Hill numbers of order 1 and 2), and functional indices (mutation frequency and nucleotide diversity). We also evaluated the pattern of nucleotide changes to investigate which of them could be the cause of the quasispecies complexity. RESULTS: CHB patients showed higher median HBV-DNA levels [5.4 logIU/mL, interquartile range (IQR) 3.5-7.9] than CHD (3.4 logIU/mL, IQR 3-7.6) (P = n.s.) or CI (3.2 logIU/mL, IQR 2.3-3.5) (P < 0.01) patients. The incidence and abundance indices indicated that HBV quasispecies complexity was significantly greater in CI than CHB. A similar trend was observed in CHD patients, although only Hill numbers of order 2 showed statistically significant differences (CHB 2.81, IQR 1.11-4.57 vs CHD 8.87, 6.56-11.18, P = 0.038). There were no significant differences in the functional indices, but CI and CHD patients also showed a trend towards greater complexity than CHB. No differences were found for any HBV quasispecies complexity indices between CHD and CI patients. G-to-A and C-to-T nucleotide changes, characteristic of APOBEC3G, were higher in CHD and CI than in CHB in genotype A haplotypes, but not in genotype D. The proportion of nt G-to-A vs A-to-G changes and C-to-T vs T-to-C changes in genotype A and D haplotypes in CHD patients showed no significant differences. In CHB and CI the results of these comparisons were dependent on HBV genotype. CONCLUSION: The lower-replication CHD and CI groups show a trend to higher quasispecies complexity than the higher-replication CHB group. The mechanisms associated with this greater complexity require elucidation.Supported by the Instituto de Salud Carlos III, grants PI15/00856 and PI17/02233; co-financed by the European Regional Development Fund (ERDF)Baishideng Publishing GroupInstitut Català de la Salut[Godoy C, Tabernero D, Sopena S, Cortese MF, González C, Casillas R, Yll M, Rando A, López-Martínez R, Rodríguez-Frías F] Unitat de patologia hepàtica, Servei de Bioquímica i Microbiologia, Hospital Universitari Vall d'Hebron, Barcelona, Spain. Universitat Autònoma de Barcelona, Barcelona, Spain. [Gregori J] Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, Madrid, Spain.Vall d'Hebron Barcelona Hospital Campus201920192019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/11351/4138http://hdl.handle.net/11351/4138Scientiareponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésWorld Journal of Gastroenterology;25(13)https://www.wjgnet.com/1007-9327/full/v25/i13/1566.htmAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:11351/41382026-05-29T05:05:01Z
score 15,812429

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