Expression analysis of vernalization and day-length response genes in barley (Hordeum vulgare L.) indicates that VRNH2 is a repressor of PPDH2 (HvFT3) under long days

38 Pag., 1 Tabl., 6 Fig.

Detalhes bibliográficos
Autores: Casao Acerete, Cristina, Igartua Arregui, Ernesto, Karsaï, Ildikó, Lasa Dolhagaray, José Manuel, Gracia Gimeno, María Pilar, Casas Cendoya, Ana María
Formato: artículo
Fecha de publicación:2011
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/35035
Acesso em linha:http://hdl.handle.net/10261/35035
Access Level:acceso abierto
Palavra-chave:Barley
Gene expression
Landraces
Mediterranean conditions
Photoperiod
vernalization
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spelling Expression analysis of vernalization and day-length response genes in barley (Hordeum vulgare L.) indicates that VRNH2 is a repressor of PPDH2 (HvFT3) under long daysCasao Acerete, CristinaIgartua Arregui, ErnestoKarsaï, IldikóLasa Dolhagaray, José ManuelGracia Gimeno, María PilarCasas Cendoya, Ana MaríaBarleyGene expressionLandracesMediterranean conditionsPhotoperiodvernalization38 Pag., 1 Tabl., 6 Fig.The response to vernalization and the expression of genes associated with responses to vernalization (VRNH1, VRNH2, and VRNH3) and photoperiod (PPDH1 and PPDH2) were analysed in four barley (Hordeum vulgare L.) lines: ‘Alexis’ (spring), ‘Plaisant’ (winter), SBCC058, and SBCC106 (Spanish inbred lines), grown under conditions of vernalization and short days (VSD) or no vernalization and long days (NVLD). The four genotypes differ in VRNH1. Their growth habits and responses to vernalization correlated with the level of expression of VRNH1 and the length of intron 1. ‘Alexis’ and ‘Plaisant’ behaved as expected. SBCC058 and SBCC106 showed an intermediate growth habit and flowered relatively late in the absence of vernalization. VRNH1 expression was induced by cold for all genotypes. Under VSD, VRNH1 expression was detected in the SBCC genotypes later than in ‘Alexis’ but earlier than in ‘Plaisant’. VRNH2 was repressed under short days while VRNH1 expression increased in parallel. VRNH3 was detected only in ‘Alexis’ under NVLD, whereas it was not expressed in plants with the active allele of VRNH2 (SBCC058 and ‘Plaisant’). Under VSD, PPDH2 was expressed in ‘Alexis’, SBCC058, and SBCC106, but it was only expressed weakly in ‘Alexis’ under NVLD. Further analysis of PPDH2 expression in two barley doubled haploid populations revealed that, under long days, HvFT3 and VRNH2 expression levels were related inversely. The timing of VRNH2 expression under a long photoperiod suggests that this gene might be involved in repression of PPDH2 and, indirectly, in the regulation of flowering time through an interaction with the day-length pathway.This study was funded by grants AGL2007-63625 and HH2008-0013 from the Spanish Ministry of Science and Technology and by the European Regional Development Fund. Germplasm from the SBCC is maintained with funding from project RFP2004-00015-00-00. MCC was supported by an I3P Predoctoral Fellowship from CSIC.Peer reviewedOxford University Press201120112011info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/35035reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1093/jxb/erq382info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/350352026-05-22T06:33:51Z
dc.title.none.fl_str_mv Expression analysis of vernalization and day-length response genes in barley (Hordeum vulgare L.) indicates that VRNH2 is a repressor of PPDH2 (HvFT3) under long days
title Expression analysis of vernalization and day-length response genes in barley (Hordeum vulgare L.) indicates that VRNH2 is a repressor of PPDH2 (HvFT3) under long days
spellingShingle Expression analysis of vernalization and day-length response genes in barley (Hordeum vulgare L.) indicates that VRNH2 is a repressor of PPDH2 (HvFT3) under long days
Casao Acerete, Cristina
Barley
Gene expression
Landraces
Mediterranean conditions
Photoperiod
vernalization
title_short Expression analysis of vernalization and day-length response genes in barley (Hordeum vulgare L.) indicates that VRNH2 is a repressor of PPDH2 (HvFT3) under long days
title_full Expression analysis of vernalization and day-length response genes in barley (Hordeum vulgare L.) indicates that VRNH2 is a repressor of PPDH2 (HvFT3) under long days
title_fullStr Expression analysis of vernalization and day-length response genes in barley (Hordeum vulgare L.) indicates that VRNH2 is a repressor of PPDH2 (HvFT3) under long days
title_full_unstemmed Expression analysis of vernalization and day-length response genes in barley (Hordeum vulgare L.) indicates that VRNH2 is a repressor of PPDH2 (HvFT3) under long days
title_sort Expression analysis of vernalization and day-length response genes in barley (Hordeum vulgare L.) indicates that VRNH2 is a repressor of PPDH2 (HvFT3) under long days
dc.creator.none.fl_str_mv Casao Acerete, Cristina
Igartua Arregui, Ernesto
Karsaï, Ildikó
Lasa Dolhagaray, José Manuel
Gracia Gimeno, María Pilar
Casas Cendoya, Ana María
author Casao Acerete, Cristina
author_facet Casao Acerete, Cristina
Igartua Arregui, Ernesto
Karsaï, Ildikó
Lasa Dolhagaray, José Manuel
Gracia Gimeno, María Pilar
Casas Cendoya, Ana María
author_role author
author2 Igartua Arregui, Ernesto
Karsaï, Ildikó
Lasa Dolhagaray, José Manuel
Gracia Gimeno, María Pilar
Casas Cendoya, Ana María
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Barley
Gene expression
Landraces
Mediterranean conditions
Photoperiod
vernalization
topic Barley
Gene expression
Landraces
Mediterranean conditions
Photoperiod
vernalization
description 38 Pag., 1 Tabl., 6 Fig.
publishDate 2011
dc.date.none.fl_str_mv 2011
2011
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/35035
url http://hdl.handle.net/10261/35035
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1093/jxb/erq382
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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