Bartonella henselae Antibodies in Serum and Oral Fluid Specimens from Cats

Cats are the primary reservoir host for Bartonella henselae(B. henselae), an etiological agent of human bartonellosis, including cat scratch disease. Although Bartonella DNA has been amplified from salivary swabs from cats, dogs and humans, we are not aware of studies investigating Bartonella antibo...

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Autores: Álvarez-Fernández, Alejandra, Breitschwerdt, Edward|||0000-0002-3506-0279, Solano Gallego, Laia|||0000-0001-8479-4896, Baxarias, Marta|||0000-0002-1830-4366, Prandi Chevalier, David|||0000-0003-4793-4209
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:237967
Acceso en línea:https://ddd.uab.cat/record/237967
https://dx.doi.org/urn:doi:10.3390/pathogens10030329
Access Level:acceso abierto
Palabra clave:Fèlids
Gats
Malalties
Bartonellosis
Feline
Serology
Immunofluorescence antibody assay
Oral fluid
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spelling Bartonella henselae Antibodies in Serum and Oral Fluid Specimens from CatsÁlvarez-Fernández, AlejandraBreitschwerdt, Edward|||0000-0002-3506-0279Solano Gallego, Laia|||0000-0001-8479-4896Baxarias, Marta|||0000-0002-1830-4366Prandi Chevalier, David|||0000-0003-4793-4209FèlidsGatsMalaltiesBartonellosisFelineSerologyImmunofluorescence antibody assayOral fluidCats are the primary reservoir host for Bartonella henselae(B. henselae), an etiological agent of human bartonellosis, including cat scratch disease. Although Bartonella DNA has been amplified from salivary swabs from cats, dogs and humans, we are not aware of studies investigating Bartonella antibodies in oral fluid (OF). Using inhouse and commercial immunofluorescence antibody assays (IFA), the objective of this study was to detect and compare antibodies against B. henselae in paired OF and serum specimens from cats. Specimens were collected from shelter and client-owned cats. For serum specimens, B. henselae seroreactivity was 78% for both the inhouse and commercial IFA assays and 56.8% for OF specimens. Comparing serum and OF specimens, there was moderate Kappa agreement (Cohen's k = 0.434) for detection of B. henselae antibodies. Oral fluid antibodies were more likely measurable in cats with high B. henselae serum antibody titers when compared with low antibody titers. In conclusion, B. henselae OF IFA antibody measurements were less sensitive compared to serum IFA measurements of ≥1:64. Oral fluid antibodies were detected more often in cats with high B. henselae serum antibody titers. Therefore, OF antibodies, detectable by IFA, is of limited utility for epidemiological or diagnostic testing in cats. 22021-01-0120212021-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/237967https://dx.doi.org/urn:doi:10.3390/pathogens10030329reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2379672026-06-06T12:50:31Z
dc.title.none.fl_str_mv Bartonella henselae Antibodies in Serum and Oral Fluid Specimens from Cats
title Bartonella henselae Antibodies in Serum and Oral Fluid Specimens from Cats
spellingShingle Bartonella henselae Antibodies in Serum and Oral Fluid Specimens from Cats
Álvarez-Fernández, Alejandra
Fèlids
Gats
Malalties
Bartonellosis
Feline
Serology
Immunofluorescence antibody assay
Oral fluid
title_short Bartonella henselae Antibodies in Serum and Oral Fluid Specimens from Cats
title_full Bartonella henselae Antibodies in Serum and Oral Fluid Specimens from Cats
title_fullStr Bartonella henselae Antibodies in Serum and Oral Fluid Specimens from Cats
title_full_unstemmed Bartonella henselae Antibodies in Serum and Oral Fluid Specimens from Cats
title_sort Bartonella henselae Antibodies in Serum and Oral Fluid Specimens from Cats
dc.creator.none.fl_str_mv Álvarez-Fernández, Alejandra
Breitschwerdt, Edward|||0000-0002-3506-0279
Solano Gallego, Laia|||0000-0001-8479-4896
Baxarias, Marta|||0000-0002-1830-4366
Prandi Chevalier, David|||0000-0003-4793-4209
author Álvarez-Fernández, Alejandra
author_facet Álvarez-Fernández, Alejandra
Breitschwerdt, Edward|||0000-0002-3506-0279
Solano Gallego, Laia|||0000-0001-8479-4896
Baxarias, Marta|||0000-0002-1830-4366
Prandi Chevalier, David|||0000-0003-4793-4209
author_role author
author2 Breitschwerdt, Edward|||0000-0002-3506-0279
Solano Gallego, Laia|||0000-0001-8479-4896
Baxarias, Marta|||0000-0002-1830-4366
Prandi Chevalier, David|||0000-0003-4793-4209
author2_role author
author
author
author
dc.subject.none.fl_str_mv Fèlids
Gats
Malalties
Bartonellosis
Feline
Serology
Immunofluorescence antibody assay
Oral fluid
topic Fèlids
Gats
Malalties
Bartonellosis
Feline
Serology
Immunofluorescence antibody assay
Oral fluid
description Cats are the primary reservoir host for Bartonella henselae(B. henselae), an etiological agent of human bartonellosis, including cat scratch disease. Although Bartonella DNA has been amplified from salivary swabs from cats, dogs and humans, we are not aware of studies investigating Bartonella antibodies in oral fluid (OF). Using inhouse and commercial immunofluorescence antibody assays (IFA), the objective of this study was to detect and compare antibodies against B. henselae in paired OF and serum specimens from cats. Specimens were collected from shelter and client-owned cats. For serum specimens, B. henselae seroreactivity was 78% for both the inhouse and commercial IFA assays and 56.8% for OF specimens. Comparing serum and OF specimens, there was moderate Kappa agreement (Cohen's k = 0.434) for detection of B. henselae antibodies. Oral fluid antibodies were more likely measurable in cats with high B. henselae serum antibody titers when compared with low antibody titers. In conclusion, B. henselae OF IFA antibody measurements were less sensitive compared to serum IFA measurements of ≥1:64. Oral fluid antibodies were detected more often in cats with high B. henselae serum antibody titers. Therefore, OF antibodies, detectable by IFA, is of limited utility for epidemiological or diagnostic testing in cats.
publishDate 2021
dc.date.none.fl_str_mv 2
2021-01-01
2021
2021-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/237967
https://dx.doi.org/urn:doi:10.3390/pathogens10030329
url https://ddd.uab.cat/record/237967
https://dx.doi.org/urn:doi:10.3390/pathogens10030329
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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