Capillary liquid-chromatography-ion trap-mass spectrometry methodology for the simultaneous quantification of four angiotensin-converting enzyme-inhibitory peptides in Prunus seed hydrolysates

Prunus genus fruit seeds are sources of highly angiotensin-l-converting enzyme (ACE)-inhibitory peptides. The presence of peptides IYSPH, IYTPH, IFSPR, and VAIP seems to be related to this activity but no previous work has demonstrated the direct relationship between the concentration of these pepti...

Descripción completa

Detalles Bibliográficos
Autores: González García, Estefanía, García López, María Concepción|||0000-0002-3383-6176, Marina Alegre, María Luisa|||0000-0002-5583-1624
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universidad de Alcalá (UAH)
Repositorio:e_Buah Biblioteca Digital Universidad de Alcalá
Idioma:inglés
OAI Identifier:oai:ebuah.uah.es:10017/44847
Acceso en línea:http://hdl.handle.net/10017/44847
https://dx.doi.org/10.1016/j.chroma.2018.02.003
Access Level:acceso abierto
Palabra clave:ACE inhibitory peptides
Quantification
Capillary-HPLC
Ion trap mass spectrometry
Prunus seeds
Química
Chemistry
id ES_eba469836bb5868423e5a01bf9dfd838
oai_identifier_str oai:ebuah.uah.es:10017/44847
network_acronym_str ES
network_name_str España
repository_id_str
spelling Capillary liquid-chromatography-ion trap-mass spectrometry methodology for the simultaneous quantification of four angiotensin-converting enzyme-inhibitory peptides in Prunus seed hydrolysatesGonzález García, EstefaníaGarcía López, María Concepción|||0000-0002-3383-6176Marina Alegre, María Luisa|||0000-0002-5583-1624ACE inhibitory peptidesQuantificationCapillary-HPLCIon trap mass spectrometryPrunus seedsQuímicaChemistryPrunus genus fruit seeds are sources of highly angiotensin-l-converting enzyme (ACE)-inhibitory peptides. The presence of peptides IYSPH, IYTPH, IFSPR, and VAIP seems to be related to this activity but no previous work has demonstrated the direct relationship between the concentration of these peptides and the antihypertensive activity of hydrolysates. This work describes the development of a method for the quantification of these peptides in Prunus seeds hydrolysates based on capillary liquid chromatography-IT-MS/MS. The analytical characteristics of the method were evaluated through the study of the linearity, LOD, LOQ presence of matrix interferences, precision, and recovery. The developed methodology was applied to the determination of the four peptides in seed hydrolysates from different Prunus genus fruits: peaches (7 varieties), plums (2 varieties), nectarines (3 varieties), apricots (2 varieties), cherry, and paraguayo. Peaches and plums seed hydrolysates yielded the highest concentrations of these peptides while paraguayo one showed the lowest concentrations. A high correlation between peptides concentrations was demonstrated suggesting that the four peptides could be released from the same seed proteins.Ministerio de Economía y CompetitividadComunidad de Madrid20182018-03-09journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/44847https://dx.doi.org/10.1016/j.chroma.2018.02.003reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available AGL2016-79010-R Desarrollo de métodos sostenibles para el aprovechamiento de subproductos de la industria alimentaria con elevado contenido proteicoComunidad de Madrid http://dx.doi.org/10.13039/100012818 Not available S2013%2FABI-3028 Estrategias avanzadas para la mejora y el control de la calidad y la seguridad de los alimentosopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-ShareAlike 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/448472026-06-18T11:13:07Z
dc.title.none.fl_str_mv Capillary liquid-chromatography-ion trap-mass spectrometry methodology for the simultaneous quantification of four angiotensin-converting enzyme-inhibitory peptides in Prunus seed hydrolysates
title Capillary liquid-chromatography-ion trap-mass spectrometry methodology for the simultaneous quantification of four angiotensin-converting enzyme-inhibitory peptides in Prunus seed hydrolysates
spellingShingle Capillary liquid-chromatography-ion trap-mass spectrometry methodology for the simultaneous quantification of four angiotensin-converting enzyme-inhibitory peptides in Prunus seed hydrolysates
González García, Estefanía
ACE inhibitory peptides
Quantification
Capillary-HPLC
Ion trap mass spectrometry
Prunus seeds
Química
Chemistry
title_short Capillary liquid-chromatography-ion trap-mass spectrometry methodology for the simultaneous quantification of four angiotensin-converting enzyme-inhibitory peptides in Prunus seed hydrolysates
title_full Capillary liquid-chromatography-ion trap-mass spectrometry methodology for the simultaneous quantification of four angiotensin-converting enzyme-inhibitory peptides in Prunus seed hydrolysates
title_fullStr Capillary liquid-chromatography-ion trap-mass spectrometry methodology for the simultaneous quantification of four angiotensin-converting enzyme-inhibitory peptides in Prunus seed hydrolysates
title_full_unstemmed Capillary liquid-chromatography-ion trap-mass spectrometry methodology for the simultaneous quantification of four angiotensin-converting enzyme-inhibitory peptides in Prunus seed hydrolysates
title_sort Capillary liquid-chromatography-ion trap-mass spectrometry methodology for the simultaneous quantification of four angiotensin-converting enzyme-inhibitory peptides in Prunus seed hydrolysates
dc.creator.none.fl_str_mv González García, Estefanía
García López, María Concepción|||0000-0002-3383-6176
Marina Alegre, María Luisa|||0000-0002-5583-1624
author González García, Estefanía
author_facet González García, Estefanía
García López, María Concepción|||0000-0002-3383-6176
Marina Alegre, María Luisa|||0000-0002-5583-1624
author_role author
author2 García López, María Concepción|||0000-0002-3383-6176
Marina Alegre, María Luisa|||0000-0002-5583-1624
author2_role author
author
dc.subject.none.fl_str_mv ACE inhibitory peptides
Quantification
Capillary-HPLC
Ion trap mass spectrometry
Prunus seeds
Química
Chemistry
topic ACE inhibitory peptides
Quantification
Capillary-HPLC
Ion trap mass spectrometry
Prunus seeds
Química
Chemistry
description Prunus genus fruit seeds are sources of highly angiotensin-l-converting enzyme (ACE)-inhibitory peptides. The presence of peptides IYSPH, IYTPH, IFSPR, and VAIP seems to be related to this activity but no previous work has demonstrated the direct relationship between the concentration of these peptides and the antihypertensive activity of hydrolysates. This work describes the development of a method for the quantification of these peptides in Prunus seeds hydrolysates based on capillary liquid chromatography-IT-MS/MS. The analytical characteristics of the method were evaluated through the study of the linearity, LOD, LOQ presence of matrix interferences, precision, and recovery. The developed methodology was applied to the determination of the four peptides in seed hydrolysates from different Prunus genus fruits: peaches (7 varieties), plums (2 varieties), nectarines (3 varieties), apricots (2 varieties), cherry, and paraguayo. Peaches and plums seed hydrolysates yielded the highest concentrations of these peptides while paraguayo one showed the lowest concentrations. A high correlation between peptides concentrations was demonstrated suggesting that the four peptides could be released from the same seed proteins.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-03-09
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10017/44847
https://dx.doi.org/10.1016/j.chroma.2018.02.003
url http://hdl.handle.net/10017/44847
https://dx.doi.org/10.1016/j.chroma.2018.02.003
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available AGL2016-79010-R Desarrollo de métodos sostenibles para el aprovechamiento de subproductos de la industria alimentaria con elevado contenido proteico
Comunidad de Madrid http://dx.doi.org/10.13039/100012818 Not available S2013%2FABI-3028 Estrategias avanzadas para la mejora y el control de la calidad y la seguridad de los alimentos
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-ShareAlike 4.0 International
http://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-ShareAlike 4.0 International
http://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:e_Buah Biblioteca Digital Universidad de Alcalá
instname:Universidad de Alcalá (UAH)
instname_str Universidad de Alcalá (UAH)
reponame_str e_Buah Biblioteca Digital Universidad de Alcalá
collection e_Buah Biblioteca Digital Universidad de Alcalá
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869423245549109248
score 15.301603

Similares en LA Referencia