Examining Topoisomers of a Snake-Venom-Derived Peptide for Improved Antimicrobial and Antitumoral Properties

Ctn[15-34], the C-terminal section of crotalicidin (Ctn), a cathelicidin from a South American pit viper, is an antimicrobial and antitumoral peptide with remarkably longer stability in human serum than the parent Ctn. In this work, a set of topoisomers of both Ctn and Ctn[15-34], including the retr...

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Detalles Bibliográficos
Autores: Carrera-Aubesart, Adam|||0000-0001-6196-1647, Defaus, Sira|||0000-0002-3106-458X, Pérez-Peinado, Clara, Sandín, Daniel|||0000-0002-2805-0208, Torrent, Marc|||0000-0001-6567-3474, Jiménez, María Ángeles|||0000-0001-6835-5850, Andreu Martínez, David|||0000-0002-6317-6666
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:266405
Acceso en línea:https://ddd.uab.cat/record/266405
https://dx.doi.org/urn:doi:10.3390/biomedicines10092110
Access Level:acceso abierto
Palabra clave:Antimicrobial peptides
Snake venom
Crotalicidin
Ctn[15-34]
Enantio-
Retro-
Retroenantio peptides
Topoisomer peptides
Antitumoral peptides
Descripción
Sumario:Ctn[15-34], the C-terminal section of crotalicidin (Ctn), a cathelicidin from a South American pit viper, is an antimicrobial and antitumoral peptide with remarkably longer stability in human serum than the parent Ctn. In this work, a set of topoisomers of both Ctn and Ctn[15-34], including the retro, enantio, and retroenantio versions, were synthesized and tested to investigate the structural requirements for activity. All topoisomers were as active as the cognate sequences against Gram-negative bacteria and tumor cells while slightly more toxic towards normal cells. More importantly, the enhanced serum stability of the D-amino-acid-containing versions suggests that such topoisomers must be preferentially considered as future antimicrobial and anticancer peptide leads.