High dietary carbohydrate inclusion by both protein and lipid replacement in gilthead sea bream. Changes in digestive and absorptive processes

The effect of high amounts of dietary carbohydrates on digestive and absorptive processes was studied in juvenile gilthead sea bream. Two trials were conducted over 14 weeks. In the first trial, sea bream were fed with isoproteic diets with lipid replaced with starch and in the second with isolipidi...

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Autores: García Meilán, Irene, Ordóñez-Grande, Borja, Valentín, Juana María, Fontanillas, Ramon, Gallardo Romero, María Ángeles
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/159729
Acceso en línea:https://hdl.handle.net/2445/159729
Access Level:acceso abierto
Palabra clave:Orada
Glúcids
Sparus aurata
Glucides
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spelling High dietary carbohydrate inclusion by both protein and lipid replacement in gilthead sea bream. Changes in digestive and absorptive processesGarcía Meilán, IreneOrdóñez-Grande, BorjaValentín, Juana MaríaFontanillas, RamonGallardo Romero, María ÁngelesOradaGlúcidsSparus aurataGlucidesThe effect of high amounts of dietary carbohydrates on digestive and absorptive processes was studied in juvenile gilthead sea bream. Two trials were conducted over 14 weeks. In the first trial, sea bream were fed with isoproteic diets with lipid replaced with starch and in the second with isolipidic diets with protein replaced with starch; in both wheat starch was included at above the established maximum levels for this fish species. At the end of each trial, intestinal alkaline protease, α-amylase and lipase activities were measured post-feeding, and alkaline phosphatase and leucine aminopeptidase activities and nutrient absorption capacities were measured under basal conditions. When lipid (23 to 17%) was replaced with starch (12 to 28%) in the 46% protein diet, a decrease in trypsin and chymotrypsin activities was found without modifying the trypsin/chymotrypsin ratio as dietary starch increases. Moreover, the pH of the intestinal content in the pyloric caeca increased and alkaline phosphatase activity and d-glucose absorption capacity decreased. Thus, compensatory mechanisms in buffer capacity, pancreatic enzyme activities and nutrient absorption capacities took place, allowing the maintenance of feed utilization and growth. Diets for the second trial were formulated with the lowest lipid content tested previously (17%) and protein (46 to 40%) was substituted with starch (19 to 39%). Lipase and trypsin activities increased as dietary starch raise, chymotrypsin activity and l-lysine absorption were not modified and amylase activity and d-glucose and L-alanine absorption decreased, but these digestive compensatory mechanisms were not sufficient, and there was a significant increase in FCR and a decrease in voluntary feed intake and SGR. This study suggests that sea bream are able to modulate the digestive process when protein requirements are met.Elsevier B.V.2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/159729Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.1016/j.aquaculture.2020.734977Aquaculture, 2020, vol. 520https://doi.org/10.1016/j.aquaculture.2020.734977cc-by-nc-nd (c) Elsevier B.V., 2020http://creativecommons.org/licenses/by-nc-nd/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1597292026-05-27T06:46:51Z
dc.title.none.fl_str_mv High dietary carbohydrate inclusion by both protein and lipid replacement in gilthead sea bream. Changes in digestive and absorptive processes
title High dietary carbohydrate inclusion by both protein and lipid replacement in gilthead sea bream. Changes in digestive and absorptive processes
spellingShingle High dietary carbohydrate inclusion by both protein and lipid replacement in gilthead sea bream. Changes in digestive and absorptive processes
García Meilán, Irene
Orada
Glúcids
Sparus aurata
Glucides
title_short High dietary carbohydrate inclusion by both protein and lipid replacement in gilthead sea bream. Changes in digestive and absorptive processes
title_full High dietary carbohydrate inclusion by both protein and lipid replacement in gilthead sea bream. Changes in digestive and absorptive processes
title_fullStr High dietary carbohydrate inclusion by both protein and lipid replacement in gilthead sea bream. Changes in digestive and absorptive processes
title_full_unstemmed High dietary carbohydrate inclusion by both protein and lipid replacement in gilthead sea bream. Changes in digestive and absorptive processes
title_sort High dietary carbohydrate inclusion by both protein and lipid replacement in gilthead sea bream. Changes in digestive and absorptive processes
dc.creator.none.fl_str_mv García Meilán, Irene
Ordóñez-Grande, Borja
Valentín, Juana María
Fontanillas, Ramon
Gallardo Romero, María Ángeles
author García Meilán, Irene
author_facet García Meilán, Irene
Ordóñez-Grande, Borja
Valentín, Juana María
Fontanillas, Ramon
Gallardo Romero, María Ángeles
author_role author
author2 Ordóñez-Grande, Borja
Valentín, Juana María
Fontanillas, Ramon
Gallardo Romero, María Ángeles
author2_role author
author
author
author
dc.subject.none.fl_str_mv Orada
Glúcids
Sparus aurata
Glucides
topic Orada
Glúcids
Sparus aurata
Glucides
description The effect of high amounts of dietary carbohydrates on digestive and absorptive processes was studied in juvenile gilthead sea bream. Two trials were conducted over 14 weeks. In the first trial, sea bream were fed with isoproteic diets with lipid replaced with starch and in the second with isolipidic diets with protein replaced with starch; in both wheat starch was included at above the established maximum levels for this fish species. At the end of each trial, intestinal alkaline protease, α-amylase and lipase activities were measured post-feeding, and alkaline phosphatase and leucine aminopeptidase activities and nutrient absorption capacities were measured under basal conditions. When lipid (23 to 17%) was replaced with starch (12 to 28%) in the 46% protein diet, a decrease in trypsin and chymotrypsin activities was found without modifying the trypsin/chymotrypsin ratio as dietary starch increases. Moreover, the pH of the intestinal content in the pyloric caeca increased and alkaline phosphatase activity and d-glucose absorption capacity decreased. Thus, compensatory mechanisms in buffer capacity, pancreatic enzyme activities and nutrient absorption capacities took place, allowing the maintenance of feed utilization and growth. Diets for the second trial were formulated with the lowest lipid content tested previously (17%) and protein (46 to 40%) was substituted with starch (19 to 39%). Lipase and trypsin activities increased as dietary starch raise, chymotrypsin activity and l-lysine absorption were not modified and amylase activity and d-glucose and L-alanine absorption decreased, but these digestive compensatory mechanisms were not sufficient, and there was a significant increase in FCR and a decrease in voluntary feed intake and SGR. This study suggests that sea bream are able to modulate the digestive process when protein requirements are met.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/159729
url https://hdl.handle.net/2445/159729
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1016/j.aquaculture.2020.734977
Aquaculture, 2020, vol. 520
https://doi.org/10.1016/j.aquaculture.2020.734977
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Elsevier B.V., 2020
http://creativecommons.org/licenses/by-nc-nd/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Elsevier B.V., 2020
http://creativecommons.org/licenses/by-nc-nd/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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