Determination of the major bioactive component of Silybum Marianum in nutricosmetics by a HPLC method with amperometric detection and UAE pretreatment
Introduction: Nutricosmetics derived from Silybum marianum, known for their anti-inflammatory and hepatoprotective properties, necessitate accurate quantification of silybin, a key bioactive component. Objectives: This study aims to develop a novel high-performance liquid chromatography (HPLC) metho...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universidad Autónoma de Madrid |
| Repositorio: | Biblos-e Archivo. Repositorio Institucional de la UAM |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.uam.es:10486/716441 |
| Acceso en línea: | http://hdl.handle.net/10486/716441 https://dx.doi.org/10.1002/pca.3478 |
| Access Level: | acceso abierto |
| Palabra clave: | Electrochemical Detection HPLC Nutricosmetics Silybin Silybum Marianum Ultrasound-Assisted Extraction Química |
| Sumario: | Introduction: Nutricosmetics derived from Silybum marianum, known for their anti-inflammatory and hepatoprotective properties, necessitate accurate quantification of silybin, a key bioactive component. Objectives: This study aims to develop a novel high-performance liquid chromatography (HPLC) method with amperometric detection (HPLC-ECD) for the precise determination of silybin. An ultrasound assisted extraction (UAE) procedure is also established for solid sample preparation prior to chromatographic analysis. Materials and Methods: Chromatographic separation of silybin was performed on a C18 column and using methanol–0.035 Mpotassium phosphate (pH 4.0) at 1.0 mL min−1 flow rate as mobile phase in gradient mode. The electrochemical detection (ECD) of silybin was carried out on a glassy carbon electrode (GCE) at +1.10 V versus Ag/AgCl. The UAE procedure for silybin extraction from solid samples was performed by 15 min sonication in an ultrasonic bath (80 kHz and 100% power) at room temperature. Results: Under the optimal chromatographic conditions, silybin diastereoisomers (silybin A and silybin B) can be separated from other S. marianum flavonolignans in less than 20 min, with a detection limit (LOD) of 0.060 mg L−1 and a reproducibility (RSD) of5%. This method was successfully applied to analyze silymarin containing products with recoveries close to 100%. Conclusions: This work presents the first HPLC method for silybin determination using an amperometric detector with a GCE. The LOD is competitive in comparison with previously published HPLC-DAD methods. This HPLC-ECD method allows silybin diastereoisomers identification without interferences of other flavonolignans present in silymarin extracts |
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