Identification of plant genes putatively involved in the perception of fungal ergosterol‐squalene
[EN] Trichoderma biocontrol strains establish a complex network of interactions with plants, in which diverse fungal molecules are involved in the recognition of these fungi as nonpathogenic organisms. These molecules act as microbial-associated molecular patterns that trigger plant responses. Previ...
| Autores: | , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2019 |
| País: | España |
| Institución: | Universidad de León |
| Repositorio: | BULERIA. Repositorio Institucional de la Universidad de León |
| OAI Identifier: | oai:buleria.unileon.es:10612/18907 |
| Acceso en línea: | https://onlinelibrary.wiley.com/doi/10.1111/jipb.12862 https://hdl.handle.net/10612/18907 |
| Access Level: | acceso abierto |
| Palabra clave: | Bioquímica Plant genes Fungal ergosterol-squalene Trichoderma Biocontrol |
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Identification of plant genes putatively involved in the perception of fungal ergosterol‐squaleneLindo Yugueros, LauraCardoza, Rosa E.Lorenzana de la Varga, AliciaCasquero Luelmo, Pedro AntonioGutiérrez , Santiago 1965-BioquímicaPlant genesFungal ergosterol-squaleneTrichodermaBiocontrol[EN] Trichoderma biocontrol strains establish a complex network of interactions with plants, in which diverse fungal molecules are involved in the recognition of these fungi as nonpathogenic organisms. These molecules act as microbial-associated molecular patterns that trigger plant responses. Previous studies have reported the importance of ergosterol produced by Trichoderma spp. for the ability of these fungi to induce plant growth and defenses. In addition, squalene, a sterol biosynthetic intermediate, seems to play an important role in these interactions. Here, we analyzed the effect of different concentrations of ergosterol and squalene on tomato (Solanum lycopersicum) growth and on the transcription level of defense- and growth-related genes. We used an RNA-seq strategy to identify several tomato genes encoding predicted pattern recognition receptor proteins or WRKY transcription factors, both of which are putatively involved in the perception and response to ergosterol and squalene. Finally, an analysis of Arabidopsis thaliana mutants lacking the genes homologous to these tomato candidates led to the identification of a WRKY40 transcription factor that negatively regulates salicylic acid-related genes and positively regulates ethylene- and jasmonate-related genes in the presence of ergosterol and squaleneSIThis work was supported by the Spanish Ministry of Economy and Competitiveness (MINECO-AGL2015–70671-C2-2-R and MICINN-RTI2018–099600-B-I00 to S.G.), and also by the Junta de Castilla y León (Spain) (LE251P18). L. Lindo was granted a fellowship by the University of León (Spain)WileyMicrobiologiaFacultad de Ciencias Biologicas y Ambientales2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://onlinelibrary.wiley.com/doi/10.1111/jipb.12862https://hdl.handle.net/10612/18907reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónIngléshttp://creativecommons.org/licenses/by-nd/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/189072026-06-24T12:43:27Z |
| dc.title.none.fl_str_mv |
Identification of plant genes putatively involved in the perception of fungal ergosterol‐squalene |
| title |
Identification of plant genes putatively involved in the perception of fungal ergosterol‐squalene |
| spellingShingle |
Identification of plant genes putatively involved in the perception of fungal ergosterol‐squalene Lindo Yugueros, Laura Bioquímica Plant genes Fungal ergosterol-squalene Trichoderma Biocontrol |
| title_short |
Identification of plant genes putatively involved in the perception of fungal ergosterol‐squalene |
| title_full |
Identification of plant genes putatively involved in the perception of fungal ergosterol‐squalene |
| title_fullStr |
Identification of plant genes putatively involved in the perception of fungal ergosterol‐squalene |
| title_full_unstemmed |
Identification of plant genes putatively involved in the perception of fungal ergosterol‐squalene |
| title_sort |
Identification of plant genes putatively involved in the perception of fungal ergosterol‐squalene |
| dc.creator.none.fl_str_mv |
Lindo Yugueros, Laura Cardoza, Rosa E. Lorenzana de la Varga, Alicia Casquero Luelmo, Pedro Antonio Gutiérrez , Santiago 1965- |
| author |
Lindo Yugueros, Laura |
| author_facet |
Lindo Yugueros, Laura Cardoza, Rosa E. Lorenzana de la Varga, Alicia Casquero Luelmo, Pedro Antonio Gutiérrez , Santiago 1965- |
| author_role |
author |
| author2 |
Cardoza, Rosa E. Lorenzana de la Varga, Alicia Casquero Luelmo, Pedro Antonio Gutiérrez , Santiago 1965- |
| author2_role |
author author author author |
| dc.contributor.none.fl_str_mv |
Microbiologia Facultad de Ciencias Biologicas y Ambientales |
| dc.subject.none.fl_str_mv |
Bioquímica Plant genes Fungal ergosterol-squalene Trichoderma Biocontrol |
| topic |
Bioquímica Plant genes Fungal ergosterol-squalene Trichoderma Biocontrol |
| description |
[EN] Trichoderma biocontrol strains establish a complex network of interactions with plants, in which diverse fungal molecules are involved in the recognition of these fungi as nonpathogenic organisms. These molecules act as microbial-associated molecular patterns that trigger plant responses. Previous studies have reported the importance of ergosterol produced by Trichoderma spp. for the ability of these fungi to induce plant growth and defenses. In addition, squalene, a sterol biosynthetic intermediate, seems to play an important role in these interactions. Here, we analyzed the effect of different concentrations of ergosterol and squalene on tomato (Solanum lycopersicum) growth and on the transcription level of defense- and growth-related genes. We used an RNA-seq strategy to identify several tomato genes encoding predicted pattern recognition receptor proteins or WRKY transcription factors, both of which are putatively involved in the perception and response to ergosterol and squalene. Finally, an analysis of Arabidopsis thaliana mutants lacking the genes homologous to these tomato candidates led to the identification of a WRKY40 transcription factor that negatively regulates salicylic acid-related genes and positively regulates ethylene- and jasmonate-related genes in the presence of ergosterol and squalene |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://onlinelibrary.wiley.com/doi/10.1111/jipb.12862 https://hdl.handle.net/10612/18907 |
| url |
https://onlinelibrary.wiley.com/doi/10.1111/jipb.12862 https://hdl.handle.net/10612/18907 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.rights.none.fl_str_mv |
http://creativecommons.org/licenses/by-nd/4.0/ info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nd/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Wiley |
| publisher.none.fl_str_mv |
Wiley |
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reponame:BULERIA. Repositorio Institucional de la Universidad de León instname:Universidad de León |
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Universidad de León |
| reponame_str |
BULERIA. Repositorio Institucional de la Universidad de León |
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BULERIA. Repositorio Institucional de la Universidad de León |
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15,300719 |