Validation of an in situ model for the formationof non-disturbed oral biofilm

The aim of this Thesis was to validate the IDODS as an appropiate model for the formation of non-disturbed oral biofilm. Firstly, a systematic review of the literature was conducted to identify all in situ studies using a specific oral device and analysed oral biofilm microbiologically. In the follo...

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Author: Prada López, Isabel
Format: doctoral thesis
Publication Date:2016
Country:España
Institution:Universidad de Santiago de Compostela (USC)
Repository:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Language:English
OAI Identifier:oai:minerva.usc.gal:10347/15006
Online Access:http://hdl.handle.net/10347/15006
Access Level:Open access
Keyword:Materias::Investigación::32 Ciencias médicas::3201 Ciencias clínicas::320103 Microbiología clínica
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spelling Validation of an in situ model for the formationof non-disturbed oral biofilmPrada López, IsabelMaterias::Investigación::32 Ciencias médicas::3201 Ciencias clínicas::320103 Microbiología clínicaThe aim of this Thesis was to validate the IDODS as an appropiate model for the formation of non-disturbed oral biofilm. Firstly, a systematic review of the literature was conducted to identify all in situ studies using a specific oral device and analysed oral biofilm microbiologically. In the following chapter, volunteers wore the device from 2 to 4 days. The characteristics (thickness, bacterial viability, covering grade and structure) of the biofilm formed on the device (PL-Biofilm) were analysed using the Confocal Laser Scanning Microscope (CLSM) in combination with LIVE/DEAD® BacLight™ staining solution and complemented with the Scanning Electron Microscope. The CLSM methodology was used to compare the PL-Biofilm versus the tooth-formed biofilm, as well as the antimicrobial activity of two oral antiseptic, (0.2 % of Chlorhexidine solution or Essential Oils), applied by two different methodologies. The 2-days PL-Biofilm and tooth-formed biofilm were also analysed by pyrosequencing of the 16S rDNA gene. The influence of the tooth-brushing protocol during the experiment was analysed with both methodologies (CLSM and pyrosequencing).Based on the results from this Thesis, the IDODS seems to be an appropiate oral biofilm model to test intraorally the immediate effect and antiplaque effect of antiseptic solutions.Tomás Carmona, InmaculadaSuárez Quintanilla, DavidUniversidade de Santiago de Compostela. Facultade de Medicina e Odontoloxía. Departamento de Cirurxía e Especialidades médico-Cirúrxicas20162016-01-0120162016-01-01doctoral thesishttp://purl.org/coar/resource_type/c_db06info:eu-repo/semantics/doctoralThesisapplication/pdfhttp://hdl.handle.net/10347/15006reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Esta obra atópase baixo unha licenza internacional Creative Commons BY-NC-ND 4.0. Calquera forma de reprodución, distribución, comunicación pública ou transformación desta obra non incluída na licenza Creative Commons BY-NC-ND 4.0 só pode ser realizada coa autorización expresa dos titulares, salvo excepción prevista pola lei. Pode acceder Vde. ao texto completo da licenza nesta ligazón: https://creativecommons.org/licenses/by-nc-nd/4.0/deed.glhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.glinfo:eu-repo/semantics/openAccessoai:minerva.usc.gal:10347/150062026-06-15T12:47:27Z
dc.title.none.fl_str_mv Validation of an in situ model for the formationof non-disturbed oral biofilm
title Validation of an in situ model for the formationof non-disturbed oral biofilm
spellingShingle Validation of an in situ model for the formationof non-disturbed oral biofilm
Prada López, Isabel
Materias::Investigación::32 Ciencias médicas::3201 Ciencias clínicas::320103 Microbiología clínica
title_short Validation of an in situ model for the formationof non-disturbed oral biofilm
title_full Validation of an in situ model for the formationof non-disturbed oral biofilm
title_fullStr Validation of an in situ model for the formationof non-disturbed oral biofilm
title_full_unstemmed Validation of an in situ model for the formationof non-disturbed oral biofilm
title_sort Validation of an in situ model for the formationof non-disturbed oral biofilm
dc.creator.none.fl_str_mv Prada López, Isabel
author Prada López, Isabel
author_facet Prada López, Isabel
author_role author
dc.contributor.none.fl_str_mv Tomás Carmona, Inmaculada
Suárez Quintanilla, David
Universidade de Santiago de Compostela. Facultade de Medicina e Odontoloxía. Departamento de Cirurxía e Especialidades médico-Cirúrxicas
dc.subject.none.fl_str_mv Materias::Investigación::32 Ciencias médicas::3201 Ciencias clínicas::320103 Microbiología clínica
topic Materias::Investigación::32 Ciencias médicas::3201 Ciencias clínicas::320103 Microbiología clínica
description The aim of this Thesis was to validate the IDODS as an appropiate model for the formation of non-disturbed oral biofilm. Firstly, a systematic review of the literature was conducted to identify all in situ studies using a specific oral device and analysed oral biofilm microbiologically. In the following chapter, volunteers wore the device from 2 to 4 days. The characteristics (thickness, bacterial viability, covering grade and structure) of the biofilm formed on the device (PL-Biofilm) were analysed using the Confocal Laser Scanning Microscope (CLSM) in combination with LIVE/DEAD® BacLight™ staining solution and complemented with the Scanning Electron Microscope. The CLSM methodology was used to compare the PL-Biofilm versus the tooth-formed biofilm, as well as the antimicrobial activity of two oral antiseptic, (0.2 % of Chlorhexidine solution or Essential Oils), applied by two different methodologies. The 2-days PL-Biofilm and tooth-formed biofilm were also analysed by pyrosequencing of the 16S rDNA gene. The influence of the tooth-brushing protocol during the experiment was analysed with both methodologies (CLSM and pyrosequencing).Based on the results from this Thesis, the IDODS seems to be an appropiate oral biofilm model to test intraorally the immediate effect and antiplaque effect of antiseptic solutions.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01
2016
2016-01-01
dc.type.none.fl_str_mv doctoral thesis
http://purl.org/coar/resource_type/c_db06
dc.type.openaire.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
dc.identifier.none.fl_str_mv http://hdl.handle.net/10347/15006
url http://hdl.handle.net/10347/15006
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.gl
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.gl
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
instname:Universidad de Santiago de Compostela (USC)
instname_str Universidad de Santiago de Compostela (USC)
reponame_str Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
collection Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
repository.name.fl_str_mv
repository.mail.fl_str_mv
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