Developmental-Based Classification of Enkephalin and Somatostatin Containing Neurons of the Chicken Central Extended Amygdala

The central extended amygdala, including the lateral bed nucleus of the stria terminalis and the central amygdala, plays a key role in stress response. To understand how the central extended amygdala regulates stress it is essential to dissect this structure at molecular, cellular and circuit levels...

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Autores: Pross, Alessandra, Hanafi-Metwalli, Alek, Desfilis, Ester, Medina Hernández, Loreta Mª
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10459.1/83658
Acesso em linha:https://doi.org/10.3389/fphys.2022.904520
http://hdl.handle.net/10459.1/83658
Access Level:acceso abierto
Palavra-chave:Bed nucleus of stria terminalis
Central amygdala
Embryonic origin
Intercalated amygdalar cells
Pain regulation
Stress regulation
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spelling Developmental-Based Classification of Enkephalin and Somatostatin Containing Neurons of the Chicken Central Extended AmygdalaPross, AlessandraHanafi-Metwalli, AlekDesfilis, EsterMedina Hernández, Loreta MªBed nucleus of stria terminalisCentral amygdalaEmbryonic originIntercalated amygdalar cellsPain regulationStress regulationThe central extended amygdala, including the lateral bed nucleus of the stria terminalis and the central amygdala, plays a key role in stress response. To understand how the central extended amygdala regulates stress it is essential to dissect this structure at molecular, cellular and circuit levels. In mammals, the central amygdala contains two distinct cell populations that become active (on cells) or inactive (off cells) during the conditioned fear response. These two cell types inhibit each other and project mainly unidirectionally to output cells, thus providing a sophisticated regulation of stress. These two cell types express either protein kinase C-delta/enkephalin or somatostatin, and were suggested to originate in different embryonic domains of the subpallium that respectively express the transcription factors Pax6 or Nkx2.1 during development. The regulation of the stress response by the central extended amygdala is poorly studied in non-mammals. Using an evolutionary developmental neurobiology approach, we previously identified several subdivisions in the central extended amygdala of chicken. These contain Pax6, Islet1 and Nkx2.1 cells that originate in dorsal striatal, ventral striatal or pallidopreoptic embryonic divisions, and also contain neurons expressing enkephalin and somatostatin. To know the origin of these cells, in this study we carried out multiple fluorescent labeling to analyze coexpression of different transcription factors with enkephalin or somatostatin. We found that many enkephalin cells coexpress Pax6 and likely derive from the dorsal striatal division, resembling the off cells of the mouse central amygdala. In contrast, most somatostatin cells coexpress Nkx2.1 and derive from the pallidal division, resembling the on cells. We also found coexpression of enkephalin and somatostatin with other transcription factors. Our results show the existence of multiple cell types in the central extended amygdala of chicken, perhaps including on/off cell systems, and set the basis for studying the role of these cells in stress regulation.Funded by grants from the Spanish Ministerio de Ciencia e Innovación (Agencia Estatal de Investigación, Grant no. PID2019-108725RB-100) and the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 812777 (H2020-MSCA-ITN-2018-812777). AHM and AP have contracts as Early-Stage Researchers paid by the H2020- MSCA-ITN-2018-812777 project. The authors declare that the funders were not involved in the study design, collection, analysis, interpretation of data, the writing of this article or the decision to submit It for publication.Frontiers Media202220222022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.3389/fphys.2022.904520http://hdl.handle.net/10459.1/83658http://hdl.handle.net/10459.1/83658reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108725RB-I00Reproducció del document publicat a: https://doi.org/10.3389/fphys.2022.904520Frontiers In Physiology, 2022, vol. 13info:eu-repo/grantAgreement/EC/H2020/812777cc-by (c) Pross, Metwalli, Desfilis and Medina, 2022info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:recercat.cat:10459.1/836582026-05-29T05:05:01Z
dc.title.none.fl_str_mv Developmental-Based Classification of Enkephalin and Somatostatin Containing Neurons of the Chicken Central Extended Amygdala
title Developmental-Based Classification of Enkephalin and Somatostatin Containing Neurons of the Chicken Central Extended Amygdala
spellingShingle Developmental-Based Classification of Enkephalin and Somatostatin Containing Neurons of the Chicken Central Extended Amygdala
Pross, Alessandra
Bed nucleus of stria terminalis
Central amygdala
Embryonic origin
Intercalated amygdalar cells
Pain regulation
Stress regulation
title_short Developmental-Based Classification of Enkephalin and Somatostatin Containing Neurons of the Chicken Central Extended Amygdala
title_full Developmental-Based Classification of Enkephalin and Somatostatin Containing Neurons of the Chicken Central Extended Amygdala
title_fullStr Developmental-Based Classification of Enkephalin and Somatostatin Containing Neurons of the Chicken Central Extended Amygdala
title_full_unstemmed Developmental-Based Classification of Enkephalin and Somatostatin Containing Neurons of the Chicken Central Extended Amygdala
title_sort Developmental-Based Classification of Enkephalin and Somatostatin Containing Neurons of the Chicken Central Extended Amygdala
dc.creator.none.fl_str_mv Pross, Alessandra
Hanafi-Metwalli, Alek
Desfilis, Ester
Medina Hernández, Loreta Mª
author Pross, Alessandra
author_facet Pross, Alessandra
Hanafi-Metwalli, Alek
Desfilis, Ester
Medina Hernández, Loreta Mª
author_role author
author2 Hanafi-Metwalli, Alek
Desfilis, Ester
Medina Hernández, Loreta Mª
author2_role author
author
author
dc.subject.none.fl_str_mv Bed nucleus of stria terminalis
Central amygdala
Embryonic origin
Intercalated amygdalar cells
Pain regulation
Stress regulation
topic Bed nucleus of stria terminalis
Central amygdala
Embryonic origin
Intercalated amygdalar cells
Pain regulation
Stress regulation
description The central extended amygdala, including the lateral bed nucleus of the stria terminalis and the central amygdala, plays a key role in stress response. To understand how the central extended amygdala regulates stress it is essential to dissect this structure at molecular, cellular and circuit levels. In mammals, the central amygdala contains two distinct cell populations that become active (on cells) or inactive (off cells) during the conditioned fear response. These two cell types inhibit each other and project mainly unidirectionally to output cells, thus providing a sophisticated regulation of stress. These two cell types express either protein kinase C-delta/enkephalin or somatostatin, and were suggested to originate in different embryonic domains of the subpallium that respectively express the transcription factors Pax6 or Nkx2.1 during development. The regulation of the stress response by the central extended amygdala is poorly studied in non-mammals. Using an evolutionary developmental neurobiology approach, we previously identified several subdivisions in the central extended amygdala of chicken. These contain Pax6, Islet1 and Nkx2.1 cells that originate in dorsal striatal, ventral striatal or pallidopreoptic embryonic divisions, and also contain neurons expressing enkephalin and somatostatin. To know the origin of these cells, in this study we carried out multiple fluorescent labeling to analyze coexpression of different transcription factors with enkephalin or somatostatin. We found that many enkephalin cells coexpress Pax6 and likely derive from the dorsal striatal division, resembling the off cells of the mouse central amygdala. In contrast, most somatostatin cells coexpress Nkx2.1 and derive from the pallidal division, resembling the on cells. We also found coexpression of enkephalin and somatostatin with other transcription factors. Our results show the existence of multiple cell types in the central extended amygdala of chicken, perhaps including on/off cell systems, and set the basis for studying the role of these cells in stress regulation.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.3389/fphys.2022.904520
http://hdl.handle.net/10459.1/83658
http://hdl.handle.net/10459.1/83658
url https://doi.org/10.3389/fphys.2022.904520
http://hdl.handle.net/10459.1/83658
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108725RB-I00
Reproducció del document publicat a: https://doi.org/10.3389/fphys.2022.904520
Frontiers In Physiology, 2022, vol. 13
info:eu-repo/grantAgreement/EC/H2020/812777
dc.rights.none.fl_str_mv cc-by (c) Pross, Metwalli, Desfilis and Medina, 2022
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by (c) Pross, Metwalli, Desfilis and Medina, 2022
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
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repository.mail.fl_str_mv
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