Postharvest heat and CO2 shocks induce changes in cuticle composition and cuticle-related gene expression in 'October Sun' peach fruit
Fruit cuticles influence greatly postharvest quality of produce. Yet, few published studies have addressed changes in cuticle composition and related gene expression from a postharvest perspective, particularly for fruit species within the Rosaceae family. Because physical treatments have proved eff...
| Autores: | , , , , |
|---|---|
| Formato: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2018 |
| País: | España |
| Recursos: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:10459.1/65227 |
| Acesso em linha: | https://doi.org/10.1016/j.postharvbio.2018.11.005 http://hdl.handle.net/10459.1/65227 |
| Access Level: | acceso abierto |
| Palavra-chave: | cuticle Gene expression heat shock Peach Postharvest |
| id |
ES_eb3458c9aa4c033d2ae68bc567acceb7 |
|---|---|
| oai_identifier_str |
oai:recercat.cat:10459.1/65227 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| spelling |
Postharvest heat and CO2 shocks induce changes in cuticle composition and cuticle-related gene expression in 'October Sun' peach fruitBelge, BurcuGoulao, Luis F.Comabella, EvaGraell i Sarle, JordiLara Ayala, IsabelcuticleGene expressionheat shockPeachPostharvestFruit cuticles influence greatly postharvest quality of produce. Yet, few published studies have addressed changes in cuticle composition and related gene expression from a postharvest perspective, particularly for fruit species within the Rosaceae family. Because physical treatments have proved effective in improving postharvest potential in some commodities, we were interested in assessing whether such benefits could arise, at some extent, from treatment-related alterations in cuticular components. In this work, commercially ripe 'October Sun' peaches were submitted to heat or CO2 shocks, and then stored at 0 °C for two weeks followed by five days at 20 °C. Wax and cutin composition was analyzed from cuticle samples isolated enzymatically from fruit skin, and expression of selected genes PpCER1, PpLACS1 and PpLipase, putatively involved in cuticle deposition, was quantified by quantitative real-time PCR. The amount (g m−2) of the main cuticular wax families identified was higher in treated fruit in comparison with the controls, particularly after the shelf life period. Treatment influence on cutin composition was less clear. Gene expression was generally impaired under cold storage, to recover to a variable extent upon transfer to 20 °C. The differential gene expression shown in response to treatments may relate to the observed compositional changes in the cuticle.This work was supported through the AGL2010-14801/ALI project, funded by the Ministerio de Ciencia e Innovación (MICINN) of Spain. B. Belge was the recipient of a FI-DGR grant from AGAUR (Generalitat de Catalunya).Elsevier2018202020192018info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://doi.org/10.1016/j.postharvbio.2018.11.005http://hdl.handle.net/10459.1/65227http://hdl.handle.net/10459.1/65227reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/grantAgreement/MICINN//AGL2010-14801Versió postprint del document publicat a: https://doi.org/10.1016/j.postharvbio.2018.11.005Postharvest Biology and Technology, 2019, núm. 148, p. 200-207cc-by-nc-nd (c) Elsevier, 2018info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/3.0/eshttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:recercat.cat:10459.1/652272026-05-29T05:05:01Z |
| dc.title.none.fl_str_mv |
Postharvest heat and CO2 shocks induce changes in cuticle composition and cuticle-related gene expression in 'October Sun' peach fruit |
| title |
Postharvest heat and CO2 shocks induce changes in cuticle composition and cuticle-related gene expression in 'October Sun' peach fruit |
| spellingShingle |
Postharvest heat and CO2 shocks induce changes in cuticle composition and cuticle-related gene expression in 'October Sun' peach fruit Belge, Burcu cuticle Gene expression heat shock Peach Postharvest |
| title_short |
Postharvest heat and CO2 shocks induce changes in cuticle composition and cuticle-related gene expression in 'October Sun' peach fruit |
| title_full |
Postharvest heat and CO2 shocks induce changes in cuticle composition and cuticle-related gene expression in 'October Sun' peach fruit |
| title_fullStr |
Postharvest heat and CO2 shocks induce changes in cuticle composition and cuticle-related gene expression in 'October Sun' peach fruit |
| title_full_unstemmed |
Postharvest heat and CO2 shocks induce changes in cuticle composition and cuticle-related gene expression in 'October Sun' peach fruit |
| title_sort |
Postharvest heat and CO2 shocks induce changes in cuticle composition and cuticle-related gene expression in 'October Sun' peach fruit |
| dc.creator.none.fl_str_mv |
Belge, Burcu Goulao, Luis F. Comabella, Eva Graell i Sarle, Jordi Lara Ayala, Isabel |
| author |
Belge, Burcu |
| author_facet |
Belge, Burcu Goulao, Luis F. Comabella, Eva Graell i Sarle, Jordi Lara Ayala, Isabel |
| author_role |
author |
| author2 |
Goulao, Luis F. Comabella, Eva Graell i Sarle, Jordi Lara Ayala, Isabel |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
cuticle Gene expression heat shock Peach Postharvest |
| topic |
cuticle Gene expression heat shock Peach Postharvest |
| description |
Fruit cuticles influence greatly postharvest quality of produce. Yet, few published studies have addressed changes in cuticle composition and related gene expression from a postharvest perspective, particularly for fruit species within the Rosaceae family. Because physical treatments have proved effective in improving postharvest potential in some commodities, we were interested in assessing whether such benefits could arise, at some extent, from treatment-related alterations in cuticular components. In this work, commercially ripe 'October Sun' peaches were submitted to heat or CO2 shocks, and then stored at 0 °C for two weeks followed by five days at 20 °C. Wax and cutin composition was analyzed from cuticle samples isolated enzymatically from fruit skin, and expression of selected genes PpCER1, PpLACS1 and PpLipase, putatively involved in cuticle deposition, was quantified by quantitative real-time PCR. The amount (g m−2) of the main cuticular wax families identified was higher in treated fruit in comparison with the controls, particularly after the shelf life period. Treatment influence on cutin composition was less clear. Gene expression was generally impaired under cold storage, to recover to a variable extent upon transfer to 20 °C. The differential gene expression shown in response to treatments may relate to the observed compositional changes in the cuticle. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018 2018 2019 2020 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion |
| format |
article |
| status_str |
acceptedVersion |
| dc.identifier.none.fl_str_mv |
https://doi.org/10.1016/j.postharvbio.2018.11.005 http://hdl.handle.net/10459.1/65227 http://hdl.handle.net/10459.1/65227 |
| url |
https://doi.org/10.1016/j.postharvbio.2018.11.005 http://hdl.handle.net/10459.1/65227 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
info:eu-repo/grantAgreement/MICINN//AGL2010-14801 Versió postprint del document publicat a: https://doi.org/10.1016/j.postharvbio.2018.11.005 Postharvest Biology and Technology, 2019, núm. 148, p. 200-207 |
| dc.rights.none.fl_str_mv |
cc-by-nc-nd (c) Elsevier, 2018 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/3.0/es http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| rights_invalid_str_mv |
cc-by-nc-nd (c) Elsevier, 2018 http://creativecommons.org/licenses/by-nc-nd/3.0/es http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
| dc.source.none.fl_str_mv |
reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| instname_str |
Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| reponame_str |
Recercat. Dipósit de la Recerca de Catalunya |
| collection |
Recercat. Dipósit de la Recerca de Catalunya |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869423207207927808 |
| score |
15,812429 |