Macrophage overexpressing NGAL ameliorated kidney fibrosis in the UUO mice model

[Background/Aims] Alternatively activated macrophages (AAM) have regenerative and anti-inflammatory characteristics. Here, we sought to evaluate whether AAM cell therapy reduces renal inflammation and fibrosis in the unilateral ureteral obstruction (UUO) mice model.

Detalles Bibliográficos
Autores: Guiteras, Roser, Solà, Anna M., Flaquer, Maria, Hotter, Georgina, Torras, Joan, Grinyó, Josep María, Cruzado, Josep María
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/176217
Acceso en línea:http://hdl.handle.net/10261/176217
Access Level:acceso abierto
Palabra clave:Chronic kidney disease
Alternatively activated macrophages
Macrophage plasticity
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spelling Macrophage overexpressing NGAL ameliorated kidney fibrosis in the UUO mice modelGuiteras, RoserSolà, Anna M.Flaquer, MariaHotter, GeorginaTorras, JoanGrinyó, Josep MaríaCruzado, Josep MaríaChronic kidney diseaseAlternatively activated macrophagesMacrophage plasticity[Background/Aims] Alternatively activated macrophages (AAM) have regenerative and anti-inflammatory characteristics. Here, we sought to evaluate whether AAM cell therapy reduces renal inflammation and fibrosis in the unilateral ureteral obstruction (UUO) mice model.[Methods] We stabilized macrophages by adenoviral vector NGAL (Neutrophil gelatinase-associated lipocalin-2) and infused them into UUO mice. To ascertain whether macrophages were capable of reaching the obstructed kidney, macrophages were stained and detected by in vivo cell tracking.[Results] We demonstrated that some infused macrophages reached the obstructed kidney and that infusion of macrophages overexpressing NGAL was associated with reduced kidney interstitial fibrosis and inflammation. This therapeutic effect was mainly associated with the phenotype and function preservation of the transferred macrophages isolated from the obstructed kidney[Conclusions] Macrophage plasticity is a major hurdle for achieving macrophage therapy success in chronic nephropathies and could be overcome by transferring lipocalin-2.This research was supported by Spanish Government Instituto de Salud Carlos III (ISCIII) grant PI12/01427, PI15/00638 and PI12/00720 Confunded by FEDER funds/European Regional Development Fund (ERDF)-a way to Build Europe-//, AMGEN grant under the auspices of The Red de Investigación Renal (European Regional Development Funds ISCIII Red Temática de Investigación Cooperativa en Salud Red de Investigación Renal; RD16/0009/0003) and SENEFRO (awarded to AS). SAF 2015-67770 (awarded to GH). AS is supported by Miguel Servet contracting system (CP08/00138).Peer reviewedS. Karger AGInstituto de Salud Carlos IIIEuropean CommissionRed Española de Investigación RenalSociedad Española de NefrologíaMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/176217reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2015-67770-Rhttps://doi.org/10.1159/000479835Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1762172026-05-22T06:33:51Z
dc.title.none.fl_str_mv Macrophage overexpressing NGAL ameliorated kidney fibrosis in the UUO mice model
title Macrophage overexpressing NGAL ameliorated kidney fibrosis in the UUO mice model
spellingShingle Macrophage overexpressing NGAL ameliorated kidney fibrosis in the UUO mice model
Guiteras, Roser
Chronic kidney disease
Alternatively activated macrophages
Macrophage plasticity
title_short Macrophage overexpressing NGAL ameliorated kidney fibrosis in the UUO mice model
title_full Macrophage overexpressing NGAL ameliorated kidney fibrosis in the UUO mice model
title_fullStr Macrophage overexpressing NGAL ameliorated kidney fibrosis in the UUO mice model
title_full_unstemmed Macrophage overexpressing NGAL ameliorated kidney fibrosis in the UUO mice model
title_sort Macrophage overexpressing NGAL ameliorated kidney fibrosis in the UUO mice model
dc.creator.none.fl_str_mv Guiteras, Roser
Solà, Anna M.
Flaquer, Maria
Hotter, Georgina
Torras, Joan
Grinyó, Josep María
Cruzado, Josep María
author Guiteras, Roser
author_facet Guiteras, Roser
Solà, Anna M.
Flaquer, Maria
Hotter, Georgina
Torras, Joan
Grinyó, Josep María
Cruzado, Josep María
author_role author
author2 Solà, Anna M.
Flaquer, Maria
Hotter, Georgina
Torras, Joan
Grinyó, Josep María
Cruzado, Josep María
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
European Commission
Red Española de Investigación Renal
Sociedad Española de Nefrología
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Chronic kidney disease
Alternatively activated macrophages
Macrophage plasticity
topic Chronic kidney disease
Alternatively activated macrophages
Macrophage plasticity
description [Background/Aims] Alternatively activated macrophages (AAM) have regenerative and anti-inflammatory characteristics. Here, we sought to evaluate whether AAM cell therapy reduces renal inflammation and fibrosis in the unilateral ureteral obstruction (UUO) mice model.
publishDate 2017
dc.date.none.fl_str_mv 2017
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/176217
url http://hdl.handle.net/10261/176217
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2015-67770-R
https://doi.org/10.1159/000479835
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv S. Karger AG
publisher.none.fl_str_mv S. Karger AG
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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