Lactate biosensing for reliable on-body sweat analysis
Wearable lactate sensors for sweat analysis are highly appealing for both the sports and healthcare fields. Electrochemical biosensing is the approach most widely used for lactate determination, and this technology generally demonstrates a linear range of response far below the expected lactate leve...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Universidad de Barcelona |
| Repositorio: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/193624 |
| Acceso en línea: | https://hdl.handle.net/2445/193624 |
| Access Level: | acceso abierto |
| Palabra clave: | Elèctrodes Biotecnologia Biosensors Electrodes Biotechnology |
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Lactate biosensing for reliable on-body sweat analysisXuan, XingPérez Ràfols, ClaraChen, ChenCuartero, MaríaCrespo, Gastón A.ElèctrodesBiotecnologiaBiosensorsElectrodesBiotechnologyBiosensorsWearable lactate sensors for sweat analysis are highly appealing for both the sports and healthcare fields. Electrochemical biosensing is the approach most widely used for lactate determination, and this technology generally demonstrates a linear range of response far below the expected lactate levels in sweat together with a high influence of pH and temperature. In this work, we present a novel analytical strategy based on the restriction of the lactate flux that reaches the enzyme lactate oxidase, which is immobilized in the biosensor core. This is accomplished by means of an outer plasticized polymeric layer containing the quaternary salt tetradodecylammonium tetrakis(4-chlorophenyl) borate (traditionally known as ETH500). Also, this layer prevents the enzyme from being in direct contact with the sample, and hence, any influence with the pH and temperature is dramatically reduced. An expanded limit of detection in the millimolar range (from 1 to 50 mM) is demonstrated with this new biosensor, in addition to an acceptable response time; appropriate repeatability, reproducibility, and reversibility (variations lower than 5% for the sensitivity); good resiliency; excellent selectivity; low drift; negligible influence of the flow rate; and extraordinary correlation (Pearson coefficient of 0.97) with a standardized method for lactate detection such as ion chromatography (through analysis of 22 sweat samples collected from 6 different subjects performing cycling or running). The developed lactate biosensor is suitable for on-body sweat lactate monitoring via a microfluidic epidermal patch additionally containing pH and temperature sensors. This applicability was demonstrated in three different body locations (forehead, thigh, and back) in a total of five on-body tests while cycling, achieving appropriate performance and validation. Moreover, the epidermal patch for lactate sensing is convenient for the analysis of sweat stimulated by iontophoresis in the subjects' arm, which is of great potential toward healthcare applications.American Chemical Society2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/193624Articles publicats en revistes (Enginyeria Química i Química Analítica)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1021/acssensors.1c01009ACS Sensors, 2021, vol. 6, p. 2763-2771https://doi.org/10.1021/acssensors.1c01009cc-by (c) Xuan, Xing et al., 2021http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1936242026-05-27T06:46:51Z |
| dc.title.none.fl_str_mv |
Lactate biosensing for reliable on-body sweat analysis |
| title |
Lactate biosensing for reliable on-body sweat analysis |
| spellingShingle |
Lactate biosensing for reliable on-body sweat analysis Xuan, Xing Elèctrodes Biotecnologia Biosensors Electrodes Biotechnology Biosensors |
| title_short |
Lactate biosensing for reliable on-body sweat analysis |
| title_full |
Lactate biosensing for reliable on-body sweat analysis |
| title_fullStr |
Lactate biosensing for reliable on-body sweat analysis |
| title_full_unstemmed |
Lactate biosensing for reliable on-body sweat analysis |
| title_sort |
Lactate biosensing for reliable on-body sweat analysis |
| dc.creator.none.fl_str_mv |
Xuan, Xing Pérez Ràfols, Clara Chen, Chen Cuartero, María Crespo, Gastón A. |
| author |
Xuan, Xing |
| author_facet |
Xuan, Xing Pérez Ràfols, Clara Chen, Chen Cuartero, María Crespo, Gastón A. |
| author_role |
author |
| author2 |
Pérez Ràfols, Clara Chen, Chen Cuartero, María Crespo, Gastón A. |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
Elèctrodes Biotecnologia Biosensors Electrodes Biotechnology Biosensors |
| topic |
Elèctrodes Biotecnologia Biosensors Electrodes Biotechnology Biosensors |
| description |
Wearable lactate sensors for sweat analysis are highly appealing for both the sports and healthcare fields. Electrochemical biosensing is the approach most widely used for lactate determination, and this technology generally demonstrates a linear range of response far below the expected lactate levels in sweat together with a high influence of pH and temperature. In this work, we present a novel analytical strategy based on the restriction of the lactate flux that reaches the enzyme lactate oxidase, which is immobilized in the biosensor core. This is accomplished by means of an outer plasticized polymeric layer containing the quaternary salt tetradodecylammonium tetrakis(4-chlorophenyl) borate (traditionally known as ETH500). Also, this layer prevents the enzyme from being in direct contact with the sample, and hence, any influence with the pH and temperature is dramatically reduced. An expanded limit of detection in the millimolar range (from 1 to 50 mM) is demonstrated with this new biosensor, in addition to an acceptable response time; appropriate repeatability, reproducibility, and reversibility (variations lower than 5% for the sensitivity); good resiliency; excellent selectivity; low drift; negligible influence of the flow rate; and extraordinary correlation (Pearson coefficient of 0.97) with a standardized method for lactate detection such as ion chromatography (through analysis of 22 sweat samples collected from 6 different subjects performing cycling or running). The developed lactate biosensor is suitable for on-body sweat lactate monitoring via a microfluidic epidermal patch additionally containing pH and temperature sensors. This applicability was demonstrated in three different body locations (forehead, thigh, and back) in a total of five on-body tests while cycling, achieving appropriate performance and validation. Moreover, the epidermal patch for lactate sensing is convenient for the analysis of sweat stimulated by iontophoresis in the subjects' arm, which is of great potential toward healthcare applications. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
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publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2445/193624 |
| url |
https://hdl.handle.net/2445/193624 |
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Inglés |
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Inglés |
| dc.relation.none.fl_str_mv |
Reproducció del document publicat a: https://doi.org/10.1021/acssensors.1c01009 ACS Sensors, 2021, vol. 6, p. 2763-2771 https://doi.org/10.1021/acssensors.1c01009 |
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cc-by (c) Xuan, Xing et al., 2021 http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess |
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cc-by (c) Xuan, Xing et al., 2021 http://creativecommons.org/licenses/by/3.0/es/ |
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openAccess |
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application/pdf |
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American Chemical Society |
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American Chemical Society |
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Articles publicats en revistes (Enginyeria Química i Química Analítica) reponame:Dipòsit Digital de la UB instname:Universidad de Barcelona |
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Universidad de Barcelona |
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Dipòsit Digital de la UB |
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Dipòsit Digital de la UB |
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